Section 1 - N-Glycan processing
Non-radioactive analysis of lipid-linked oligosaccharide compositions by fluorophore-assisted carbohydrate electrophoresis (FACE).
Identification of N-glycan-binding proteins for E3 ubiquitin ligases.
Familyi 47 á-mannosidases in N-glycan processing.
A Cytoplasmic Peptide: N-glycanase.
Section 2 - Structural Analysis
Glycomic profiling of cells and tissues by mass spectrometry – fingerprinting and sequencing methodologies.
Structural analysis of sialyl N-glycan using pyridylamination and chromatography followed by multistage tandem mass spectrometry.
Determination of glycosylation sites and disulfide bond structures using LC/ESI-MS/MS analysis.
Identification of O-GlcNAc sites on proteins.
Section 3 - Carbohydrate Synthesis and Antibiotics
Chemoenzymatic synthesis of glycan libraries.
Glycoconjugate vaccines against Haemophilus influenzae tybe b.
Assay of human gastric mucin as a natural antibiotic against Helicobacter pylori.
Molecular contacts between antibiotics and the 30S ribosomal particle.
Mechanism-based inhibitors to probe transitional states of glycoside hydrolases.
Regulating cell surface glycosylation with a small molecule switch.
Metabolic labeling of glycans with azido sugars for visualization and glycoproteomics.
Section 4 - Carbohydrate Ligand Specificity
Functional Proteomic Profiling of Glycan Processing Enzymes.
Oligosaccharide Microarrays to Map Interactions of Carbohydrates in Biological Systems.
Identification of Ligand Specificities for Glycan-binding Proteins Using Glycan Arrays.
High-throughput analysis of lectin-oligosaccharide interactions by automated frontal affinity chromatograph