COVID-19 Update: We are currently shipping orders daily. However, due to transit disruptions in some geographies, deliveries may be delayed. To provide all customers with timely access to content, we are offering 50% off Science and Technology Print & eBook bundle options. Terms & conditions.
Glycobiology - 1st Edition - ISBN: 9780121828202, 9780080468990

Glycobiology, Volume 415

1st Edition

Editor: Minoru Fukuda
eBook ISBN: 9780080468990
Hardcover ISBN: 9780121828202
Imprint: Academic Press
Published Date: 1st November 2006
Page Count: 424
Sales tax will be calculated at check-out Price includes VAT/GST
Price includes VAT/GST

Institutional Subscription

Secure Checkout

Personal information is secured with SSL technology.

Free Shipping

Free global shipping
No minimum order.

Table of Contents

Section 1 - N-Glycan processing
Non-radioactive analysis of lipid-linked oligosaccharide compositions by fluorophore-assisted carbohydrate electrophoresis (FACE).
Identification of N-glycan-binding proteins for E3 ubiquitin ligases.
Familyi 47 á-mannosidases in N-glycan processing.
A Cytoplasmic Peptide: N-glycanase.

Section 2 - Structural Analysis
Glycomic profiling of cells and tissues by mass spectrometry – fingerprinting and sequencing methodologies.
Structural analysis of sialyl N-glycan using pyridylamination and chromatography followed by multistage tandem mass spectrometry.
Determination of glycosylation sites and disulfide bond structures using LC/ESI-MS/MS analysis.
Identification of O-GlcNAc sites on proteins.

Section 3 - Carbohydrate Synthesis and Antibiotics
Chemoenzymatic synthesis of glycan libraries.
Glycoconjugate vaccines against Haemophilus influenzae tybe b.
Assay of human gastric mucin as a natural antibiotic against Helicobacter pylori.
Molecular contacts between antibiotics and the 30S ribosomal particle.
Mechanism-based inhibitors to probe transitional states of glycoside hydrolases.
Regulating cell surface glycosylation with a small molecule switch.
Metabolic labeling of glycans with azido sugars for visualization and glycoproteomics.

Section 4 - Carbohydrate Ligand Specificity
Functional Proteomic Profiling of Glycan Processing Enzymes.
Oligosaccharide Microarrays to Map Interactions of Carbohydrates in Biological Systems.
Identification of Ligand Specificities for Glycan-binding Proteins Using Glycan Arrays.
High-throughput analysis of lectin-oligosaccharide interactions by automated frontal affinity chromatography.
Preparation of Neoglycolipids with Ring-Closed Cores via Chemoselective Oxime-Ligation for Microarray Analysis of Carbohydrate-Protein Interactions.
Development of a lectin microarray based on an evanescent-field fluorescence principle.


In the past decade, there has been an explosion of progress in understanding the roles of carbohydrates in biological systems. This explosive progress was made with the efforts in determining the roles of carbohydrates in immunology, neurobiology and many other disciplines, examining each unique system and employing new technology. This volume represents the first of three in the Methods in Enzymology series, including Glycomics (vol. 416) and Functional Glycomics (vol. 417), dedicated to disseminating information on methods in determining the biological roles of carbohydrates. These books are designed to provide an introduction of new methods to a large variety of readers who would like to participate in and contribute to the advancement of glycobiology. The methods covered include structural analysis of carbohydrates, biological and chemical synthesis of carbohydrates, expression and determination of ligands for carbohydrate-binding proteins, gene expression profiling including micro array, and generation of gene knockout mice and their phenotype analyses.


Graduate students, researchers in academics and industry studying biochemistry and glycobiology.


No. of pages:
© Academic Press 2006
1st November 2006
Academic Press
eBook ISBN:
Hardcover ISBN:

Ratings and Reviews

About the Editor

Minoru Fukuda