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Section I. Nitric Oxide: Chemical and Analytical Methods
The Chemistry of Nitric Oxide and Related Species
Martin H. Hughes
Delivery of nitric oxide for analysis of the function of cytochrome c’.
Lindsay J. Cole, Wilhelmina M. Huston, and James W.B. Moir
The preparation and purification of NO gas and the use of NO releasers. The application of NO donors and other agents of nitrosative stress in biological systems
Rubina G. Aga and Martin N. Hughes
The Chemistry of Peroxynitrite – Implications for Biological Activity
Sara Goldstein and Gabor Merényi
Nitric Oxide (NO) Selective Electrodes
Ian R. Davies, Xueji Zhang
NO, N2O and O2 Reaction Kinetics: Scope and Limitations of the Clark Electrode
L.A.M. Pouvreau, M. J. F. Strampraad, S. Van Berloo, J. H. Kattenberg and S. de Vries
Chemiluminescence quantification of Nitric oxide and its derivatives in liquid samples
Jay R. Laver, Tânia M. Stevanin, Robert C. Read
Section II. Bacterial and Archaeal Hemoglobins
Interactions of nitric oxide with hemoglobin: from microbes to man
Michael Angelo, Alfred Hausladen and Jonathan S. Stamler
Expression and purification of E. coli Hmp
Robert K. Poole
Structural studies on flavohemoglobins
Andrea Ilari and Alberto Boffi
Flavohemoglobin of Staphylococcus aureus
Lígia S. Nobre, Vera L. Gonçalves and Lígia M. Saraiva
Assay and Characterization of the Nitric Oxide Dioxygenase Activity of (Flavo)Hemoglobins
Paul R. Gardner
Globin Interactions with Lipids and Membranes
Antonio Di Giulio and Alessandra Bonamore
Assessment of Biotechnologically Relevant Characteristics of Heterologous Hemoglobins in Escherichia coli
Pauli T. Kallio, Christian J. T. Bollinger, Taija Koskenkorva, and Alexander D. Frey
Applications of the Vitreoscilla hemoglobin (VHb) gene (vgb) for improved microbial fermentation processes
Xiao-Xing WEI, Guo-Qiang CHEN
Expression and Purification of Cgb and Ctb, the NO-Inducible Globins of the Foodborne Bacterial Pathogen Campylobacter jejuni
James L. Pickford, Laura Wainwright, Guanghui Wu, Robert K. Poole
Mapping Heme-Ligand Tunnel in Group I Truncated(2/2) Hemoglobins
Alessandra Pesce, Mario Milani, Marco Nardini and Martino Bolognesi
Scavenging of reactive nitrogen species by mycobacterial truncated hemoglobins
Paolo Ascenzi and Paolo Visca
Section III. Other Hemoglobins
Expression, purification and crystallisation of neuro-and cytoglobin
Sylvia Dewilde, Kirsten Mees, Laurent Coger, Christophe Lechauve, Michael C. Marden, Alexandra Pesce, Martino Bolognesi, Luc Monees
Measurement of distal histidine coordination equilibrium and kinetics in hexacoordinate hemoglobins.
Benoit J. Smagghe, Puspita Halder, and Mark S. Hargrove
Purification of Class 1 Plant Hemoglobins and Examination of their Functional Properties
Abir U. Igamberdiev and Robert D. Hill
Use of in silico (computer) methods to predict and analyze the tertiary structure of plant hemoglobins
Sabarinathan Kuttalingam Gopalasubramaniam, Verónica Garrocho-Villegas, Genoveva Bustos Rivera, Nina Pastor and Raúl Arredondo-Peter
A Self-induction Method to Produce High Quantities of Recombinant Functional Flavo-Leghemoglobin Reductase.
Estibaliz Urarte, Iñigo Auzmendi, Selene Rol, Idoia Ariz, Pedro Aparicio-Tejo, Raúl Arredondo-Peter and Jose F. Moran
Spectroscopic and crystallographic characterization of bis-histidyl adducts in tetrameric hemoglobins
Alessandro Vergara, Luigi Vitagliano, Cinzia Verde, Guido di Prisco, Lelio Mazzarella
Dinitrosyl Iron Complexes Bound with Haemoglobin as Markers of Oxidative Stress
Konstantin B. Shumaev, Olga V. Kosmachevskaya, Alexandr A. Timoshin, Anatoly F. Vanin and Alexey F. Topunov
Linked analysis of large cooperative, allosteric systems: the case of the giant HBL hemoglobins
Nadja Hellmann, Roy E. Weberb and Heinz Decker
Mass Mapping of Large Globin Complexes by Scanning Transmission Electron Microscopy
Joseph S. Wall, Martha N. Simon, Beth Y. Lin, Serge N. Vinogradov
Mini-Hemoglobins from Nemertean Worms
Thomas L. Vandergon and Austen F. Riggs
Comparative and Evolutionary Genomics of Globin Genes in Fish
Enrico Negrisolo, Luca Bargelloni, Tomaso Patarnello, Catherine Ozouf-Costaz, Eva Pisano, Guido di Prisco, Cinzia Verde
Inferring Evolution of Fish Proteins: The Globin Case Study
Agnes Dettai, Guideo de Prisco, Guillaume Lecointre, Elio Parisi, Cinzia Verde
Tracing Globin Phylogeny Using PSI-BLAST Searches
Based on Groups of Sequences
Serge N. Vinogradov
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 400 volumes (all of them still in print), the series contains much material still relevant today—truly an essential publication for researchers in all fields of life sciences.
Methods in Enzymology is now available online at ScienceDirect — full-text online of volumes 1 onwards. For more information about the Elsevier Book Series on ScienceDirect Program, please visit:
This volume features methods for the study of globin and other nitric oxide-reactive proteins.
Researchers in academics and industry studying biochemistry.
- No. of pages:
- © Academic Press 2008
- 5th February 2008
- Academic Press
- eBook ISBN:
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Professor Robert Poole is West Riding Professor of Microbiology at the University of Sheffield. He has >35 years’ experience of bacterial physiology and bioenergetics, in particular O2-, CO- and NO-reactive proteins, and has published >300 papers (h=48, 2013). He was Chairman of the Plant and Microbial Sciences Committee of the UK Biotechnology and Biological Sciences Research Council and has held numerous grants from BBSRC, the Wellcome and Leverhulme Trusts and the EC. He coordinates an international SysMO systems biology consortium. He published pioneering studies of bacterial oxidases and globins and discovered the bacterial flavohaemoglobin gene (hmp) and its function in NO detoxification He recently published the first systems analyses of responses of bacteria to novel carbon monoxide-releasing molecules (CORMs) and is a world leader in NO, CO and CORM research.
West Riding Professor of Microbiology, Department of Molecular Biology and Biotechnology, University of Sheffield, UK
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