Description

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 400 volumes (all of them still in print), the series contains much material still relevant today—truly an essential publication for researchers in all fields of life sciences. Methods in Enzymology is now available online at ScienceDirect — full-text online of volumes 1 onwards. For more information about the Elsevier Book Series on ScienceDirect Program, please visit: http://www.info.sciencedirect.com/bookseries/ This volume features methods for the study of globin and other nitric oxide-reactive proteins.

Readership

Researchers in academics and industry studying biochemistry.

Table of Contents

Section I. Nitric Oxide: Chemical and Analytical Methods Chapter 1 The Chemistry of Nitric Oxide and Related Species Martin H. Hughes Chapter 2 Delivery of nitric oxide for analysis of the function of cytochrome c’. Lindsay J. Cole, Wilhelmina M. Huston, and James W.B. Moir Chapter 3 The preparation and purification of NO gas and the use of NO releasers. The application of NO donors and other agents of nitrosative stress in biological systems Rubina G. Aga and Martin N. Hughes Chapter 4 The Chemistry of Peroxynitrite – Implications for Biological Activity Sara Goldstein and Gabor Merényi Chapter 5 Nitric Oxide (NO) Selective Electrodes Ian R. Davies, Xueji Zhang Chapter 6 NO, N2O and O2 Reaction Kinetics: Scope and Limitations of the Clark Electrode L.A.M. Pouvreau, M. J. F. Strampraad, S. Van Berloo, J. H. Kattenberg and S. de Vries Chapter 7 Chemiluminescence quantification of Nitric oxide and its derivatives in liquid samples Jay R. Laver, Tânia M. Stevanin, Robert C. Read Section II. Bacterial and Archaeal Hemoglobins Chapter 8 Interactions of nitric oxide with hemoglobin: from microbes to man Michael Angelo, Alfred Hausladen and Jonathan S. Stamler Chapter 9 Expression and purification of E. coli Hmp Robert K. Poole Chapter 10 Structural studies on flavohemoglobins Andrea Ilari and Alberto Boffi Chapter 11 Flavohemoglobin of Staphylococcus aureus Lígia S.

Details

No. of pages:
672
Language:
English
Copyright:
© 2008
Published:
Imprint:
Academic Press
eBook ISBN:
9780080560441
Print ISBN:
9780123742773

About the editor

Robert K. Poole

Professor Robert Poole is West Riding Professor of Microbiology at the University of Sheffield. He has >35 years’ experience of bacterial physiology and bioenergetics, in particular O2-, CO- and NO-reactive proteins, and has published >300 papers (h=48, 2013). He was Chairman of the Plant and Microbial Sciences Committee of the UK Biotechnology and Biological Sciences Research Council and has held numerous grants from BBSRC, the Wellcome and Leverhulme Trusts and the EC. He coordinates an international SysMO systems biology consortium. He published pioneering studies of bacterial oxidases and globins and discovered the bacterial flavohaemoglobin gene (hmp) and its function in NO detoxification He recently published the first systems analyses of responses of bacteria to novel carbon monoxide-releasing molecules (CORMs) and is a world leader in NO, CO and CORM research.

Affiliations and Expertise

University of Sheffield, UK