Globins and Other Nitric Oxide-Reactive Proteins, Part A

Globins and Other Nitric Oxide-Reactive Proteins, Part A

1st Edition - February 5, 2008

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  • Editor: Robert K. Poole
  • eBook ISBN: 9780080560441
  • Hardcover ISBN: 9780123742773

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The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 400 volumes (all of them still in print), the series contains much material still relevant today—truly an essential publication for researchers in all fields of life sciences. Methods in Enzymology is now available online at ScienceDirect — full-text online of volumes 1 onwards. For more information about the Elsevier Book Series on ScienceDirect Program, please visit: volume features methods for the study of globin and other nitric oxide-reactive proteins.


Researchers in academics and industry studying biochemistry.

Table of Contents

  • Section I. Nitric Oxide: Chemical and Analytical Methods

    Chapter 1
    The Chemistry of Nitric Oxide and Related Species
    Martin H. Hughes

    Chapter 2
    Delivery of nitric oxide for analysis of the function of cytochrome c’.
    Lindsay J. Cole, Wilhelmina M. Huston, and James W.B. Moir

    Chapter 3
    The preparation and purification of NO gas and the use of NO releasers. The application of NO donors and other agents of nitrosative stress in biological systems
    Rubina G. Aga and Martin N. Hughes

    Chapter 4
    The Chemistry of Peroxynitrite – Implications for Biological Activity
    Sara Goldstein and Gabor Merényi

    Chapter 5
    Nitric Oxide (NO) Selective Electrodes
    Ian R. Davies, Xueji Zhang

    Chapter 6
    NO, N2O and O2 Reaction Kinetics: Scope and Limitations of the Clark Electrode
    L.A.M. Pouvreau, M. J. F. Strampraad, S. Van Berloo, J. H. Kattenberg and S. de Vries

    Chapter 7
    Chemiluminescence quantification of Nitric oxide and its derivatives in liquid samples
    Jay R. Laver, Tânia M. Stevanin, Robert C. Read

    Section II. Bacterial and Archaeal Hemoglobins

    Chapter 8
    Interactions of nitric oxide with hemoglobin: from microbes to man
    Michael Angelo, Alfred Hausladen and Jonathan S. Stamler

    Chapter 9
    Expression and purification of E. coli Hmp
    Robert K. Poole

    Chapter 10
    Structural studies on flavohemoglobins
    Andrea Ilari and Alberto Boffi

    Chapter 11
    Flavohemoglobin of Staphylococcus aureus
    Lígia S. Nobre, Vera L. Gonçalves and Lígia M. Saraiva

    Chapter 12
    Assay and Characterization of the Nitric Oxide Dioxygenase Activity of (Flavo)Hemoglobins
    Paul R. Gardner

    Chapter 13
    Globin Interactions with Lipids and Membranes
    Antonio Di Giulio and Alessandra Bonamore

    Chapter 14
    Assessment of Biotechnologically Relevant Characteristics of Heterologous Hemoglobins in Escherichia coli
    Pauli T. Kallio, Christian J. T. Bollinger, Taija Koskenkorva, and Alexander D. Frey

    Chapter 15
    Applications of the Vitreoscilla hemoglobin (VHb) gene (vgb) for improved microbial fermentation processes
    Xiao-Xing WEI, Guo-Qiang CHEN

    Chapter 16
    Expression and Purification of Cgb and Ctb, the NO-Inducible Globins of the Foodborne Bacterial Pathogen Campylobacter jejuni
    James L. Pickford, Laura Wainwright, Guanghui Wu, Robert K. Poole

    Chapter 17
    Mapping Heme-Ligand Tunnel in Group I Truncated(2/2) Hemoglobins
    Alessandra Pesce, Mario Milani, Marco Nardini and Martino Bolognesi

    Chapter 18
    Scavenging of reactive nitrogen species by mycobacterial truncated hemoglobins
    Paolo Ascenzi and Paolo Visca

    Section III. Other Hemoglobins

    Chapter 19
    Expression, purification and crystallisation of neuro-and cytoglobin
    Sylvia Dewilde, Kirsten Mees, Laurent Coger, Christophe Lechauve, Michael C. Marden, Alexandra Pesce, Martino Bolognesi, Luc Monees

    Chapter 20
    Measurement of distal histidine coordination equilibrium and kinetics in hexacoordinate hemoglobins.
    Benoit J. Smagghe, Puspita Halder, and Mark S. Hargrove

    Chapter 21
    Purification of Class 1 Plant Hemoglobins and Examination of their Functional Properties
    Abir U. Igamberdiev and Robert D. Hill

    Chapter 22
    Use of in silico (computer) methods to predict and analyze the tertiary structure of plant hemoglobins
    Sabarinathan Kuttalingam Gopalasubramaniam, Verónica Garrocho-Villegas, Genoveva Bustos Rivera, Nina Pastor and Raúl Arredondo-Peter

    Chapter 23
    A Self-induction Method to Produce High Quantities of Recombinant Functional Flavo-Leghemoglobin Reductase.
    Estibaliz Urarte, Iñigo Auzmendi, Selene Rol, Idoia Ariz, Pedro Aparicio-Tejo, Raúl Arredondo-Peter and Jose F. Moran

    Chapter 24
    Spectroscopic and crystallographic characterization of bis-histidyl adducts in tetrameric hemoglobins
    Alessandro Vergara, Luigi Vitagliano, Cinzia Verde, Guido di Prisco, Lelio Mazzarella

    Chapter 25
    Dinitrosyl Iron Complexes Bound with Haemoglobin as Markers of Oxidative Stress
    Konstantin B. Shumaev, Olga V. Kosmachevskaya, Alexandr A. Timoshin, Anatoly F. Vanin and Alexey F. Topunov

    Chapter 26
    Linked analysis of large cooperative, allosteric systems: the case of the giant HBL hemoglobins
    Nadja Hellmann, Roy E. Weberb and Heinz Decker

    Chapter 27
    Mass Mapping of Large Globin Complexes by Scanning Transmission Electron Microscopy
    Joseph S. Wall, Martha N. Simon, Beth Y. Lin, Serge N. Vinogradov

    Chapter 28
    Mini-Hemoglobins from Nemertean Worms
    Thomas L. Vandergon and Austen F. Riggs

    Chapter 29
    Comparative and Evolutionary Genomics of Globin Genes in Fish
    Enrico Negrisolo, Luca Bargelloni, Tomaso Patarnello, Catherine Ozouf-Costaz, Eva Pisano, Guido di Prisco, Cinzia Verde

    Chapter 30
    Inferring Evolution of Fish Proteins: The Globin Case Study
    Agnes Dettai, Guideo de Prisco, Guillaume Lecointre, Elio Parisi, Cinzia Verde

    Chapter 31
    Tracing Globin Phylogeny Using PSI-BLAST Searches
    Based on Groups of Sequences
    Serge N. Vinogradov

Product details

  • No. of pages: 672
  • Language: English
  • Copyright: © Academic Press 2008
  • Published: February 5, 2008
  • Imprint: Academic Press
  • eBook ISBN: 9780080560441
  • Hardcover ISBN: 9780123742773

About the Editor

Robert K. Poole

Robert K. Poole
Professor Robert Poole is West Riding Professor of Microbiology at the University of Sheffield. He has >35 years’ experience of bacterial physiology and bioenergetics, in particular O2-, CO- and NO-reactive proteins, and has published >300 papers (h=48, 2013). He was Chairman of the Plant and Microbial Sciences Committee of the UK Biotechnology and Biological Sciences Research Council and has held numerous grants from BBSRC, the Wellcome and Leverhulme Trusts and the EC. He coordinates an international SysMO systems biology consortium. He published pioneering studies of bacterial oxidases and globins and discovered the bacterial flavohaemoglobin gene (hmp) and its function in NO detoxification He recently published the first systems analyses of responses of bacteria to novel carbon monoxide-releasing molecules (CORMs) and is a world leader in NO, CO and CORM research.

Affiliations and Expertise

West Riding Professor of Microbiology, Department of Molecular Biology and Biotechnology, University of Sheffield, UK

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