Section I. Nitric Oxide: Chemical and Analytical Methods
Chapter 1 The Chemistry of Nitric Oxide and Related Species Martin H. Hughes
Chapter 2 Delivery of nitric oxide for analysis of the function of cytochrome c’. Lindsay J. Cole, Wilhelmina M. Huston, and James W.B. Moir
The preparation and purification of NO gas and the use of NO releasers. The application of NO donors and other agents of nitrosative stress in biological systems
Rubina G. Aga and Martin N. Hughes
Chapter 4 The Chemistry of Peroxynitrite – Implications for Biological Activity Sara Goldstein and Gabor Merényi
Chapter 5 Nitric Oxide (NO) Selective Electrodes Ian R. Davies, Xueji Zhang
Chapter 6 NO, N2O and O2 Reaction Kinetics: Scope and Limitations of the Clark Electrode L.A.M. Pouvreau, M. J. F. Strampraad, S. Van Berloo, J. H. Kattenberg and S. de Vries
Chapter 7 Chemiluminescence quantification of Nitric oxide and its derivatives in liquid samples Jay R. Laver, Tânia M. Stevanin, Robert C. Read
Section II. Bacterial and Archaeal Hemoglobins
Chapter 8 Interactions of nitric oxide with hemoglobin: from microbes to man Michael Angelo, Alfred Hausladen and Jonathan S. Stamler
Chapter 9 Expression and purification of E. coli Hmp Robert K. Poole
Chapter 10 Structural studies on flavohemoglobins Andrea Ilari and Alberto Boffi
Chapter 11 Flavohemoglobin of Staphylococcus aureus Lígia S.
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This volume features methods for the study of globin and other nitric oxide-reactive proteins.
Researchers in academics and industry studying biochemistry.
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- 5th February 2008
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Professor Robert Poole is West Riding Professor of Microbiology at the University of Sheffield. He has >35 years’ experience of bacterial physiology and bioenergetics, in particular O2-, CO- and NO-reactive proteins, and has published >300 papers (h=48, 2013). He was Chairman of the Plant and Microbial Sciences Committee of the UK Biotechnology and Biological Sciences Research Council and has held numerous grants from BBSRC, the Wellcome and Leverhulme Trusts and the EC. He coordinates an international SysMO systems biology consortium. He published pioneering studies of bacterial oxidases and globins and discovered the bacterial flavohaemoglobin gene (hmp) and its function in NO detoxification He recently published the first systems analyses of responses of bacteria to novel carbon monoxide-releasing molecules (CORMs) and is a world leader in NO, CO and CORM research.
University of Sheffield, UK