Description

This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. The volume covers ghrelin, and has chapters on such topics as orphan gpcrs and methods for identifying their ligands, ghrelin o-acyltransferase assays and inhibition, and thermogenic characterization of ghrelin receptor null mice.

Key Features

  • Contains quality chapters authored by leaders in the field
  • Has chapters on such topics as orphan gpcrs and methods for identifying their ligands, ghrelin o-acyltransferase assays and inhibition, and thermogenic characterization of ghrelin receptor null mice

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of Contents

History of GHS and ghrelin

  1. History To The Discovery Of Ghrelin
  2. Cyril Y. Bowers

  3. Orphan Gpcrs And Methods For Identifying Their Ligands
  4. Morikatsu Yoshida, Mikiya Miyazato, and Kenji Kangawa

  5. Purification Of Rat And Human Ghrelins
  6. Masayasu Kojima, Hiroshi Hosoda and Kenji Kangawa

  7. Variety of Acyl Modifications in Mammalian Ghrelins
  8. Takanori Ida

  9. Determination Of Non-Mammalian Ghrelin
  10. Hiroyuki Kaiya, Hiroshi Hosoda, Kenji Kangawa, Mikiya Miyazato

    Molecular forms of ghrelin and Measuring of the concentrations

  11. Morphological Analysis Of Ghrelin Neurons In The Hypothalamus
  12. Haruaki Kageyama, Fumiko Takenoya and Seiji Shioda

  13. High-Performance Liquid Chromatography Analysis Of Hypothalamic Ghrelin
  14. Takahiro Sato and Masayasu Kojima

  15. Standard Sample Collections For Blood Ghrelin Measurements
  16. Hiroshi Hosoda and Kenji Kangawa

    Enzymes for processing of ghrelin

  17. From Ghrelin to Ghrelin’s O Acyl Transferase
  18. Jesus A. Gutierrez, Jill A. Willency, Michael D. Knierman, Tamer Coskun, Patricia J. Solenberg, Doug R. Perkins, Richard E. Higgs, and John E. Hale.

  19. Enzymatic Characterization Of Goat, Ghrelin O-Acyltransferase
  20. Hideko Ohgusu, Tomoko Takahashi and Masayasu Kojima

  21. The Study Of Ghrelin Deacylation Enzymes
  22. Motoyasu Satou and Hiroyuki Sugimoto

    Synthesis of ghrelin agonists and antagonists

  23. SynthesisOf Ghrelin:Chemical Synthesis And Semi-Synthesis For Large-Scale Preparation Of Modified Peptides

Details

No. of pages:
512
Language:
English
Copyright:
© 2012
Published:
Imprint:
Academic Press
Print ISBN:
9780123812728
Electronic ISBN:
9780123812735