Description

This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. The volume covers ghrelin, and has chapters on such topics as orphan gpcrs and methods for identifying their ligands, ghrelin o-acyltransferase assays and inhibition, and thermogenic characterization of ghrelin receptor null mice.

Key Features

  • Contains quality chapters authored by leaders in the field
  • Has chapters on such topics as orphan gpcrs and methods for identifying their ligands, ghrelin o-acyltransferase assays and inhibition, and thermogenic characterization of ghrelin receptor null mice

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of Contents

Series Page

Contributors

Preface

Methods in Enzymology

Chapter One History to the Discovery of Ghrelin

1 Introduction/Pre-GHRP Studies

2 GHRP Beginning

3 GHRP-6 Active In Vitro and In Vivo (1982)

4 GHRH Isolation and Identification (1982)

5 GHRP Versus GHRH (1981–1984)

6 GHRP Reflects Activity of a New Hormone and New Receptor—Hypothalamic–Pituitary Models (1984–1991)

7 GHRP/GHS Receptor Assays (1989–2000)

8 GHS/GHRP Receptor (1996–1998)

9 Clinical Results in Humans

10 Summary/Conclusion

Chapter Two Orphan GPCRs and Methods for Identifying Their Ligands

1 Introduction

2 Strategies for Identifying Ligands of Orphan GPCRs

3 Selection of Target Orphan GPCRs

4 Recombinant Expression Systems for Orphan GPCRs

5 Sources of Potential Ligands of Orphan GPCRs

6 Functional Assays for Screening Candidate Ligands of Orphan GPCRs

7 Summary

Chapter Three Purification of Rat and Human Ghrelins

1 Introduction

2 GHS and Its Receptor

3 Purification of Rat Ghrelin

4 Purification of Human Ghrelin

5 Purification of Rat des-Gln14-ghrelin

6 Obestatin, an Anorexigenic Hormone or a Digested Peptide Fragment from a Ghrelin Precursor?

7 Conclusion

Chapter Four Variety of Acyl Modifications in Mammalian Ghrelins

1 Introduction

2 Purification of Mammalian Ghrelin

3 GH-Releasing Activity of Ghrelin in Mammals

4 Summary

Chapter Five Determination of Nonmammalian Ghrelin

1 Introduction

2 Purification of Ghrelin

3 Measurement of Ghrelin Activity

4 CDNA Cloning and Determination of Amino Acid Sequence of Prepro-Ghrelin

5 Determination of Species of Fatty Acid Modification by Mass Spectrometric Analysis

6 Summary

Chapter Six Morphological Analysis of Ghrelin Ne

Details

No. of pages:
512
Language:
English
Copyright:
© 2012
Published:
Imprint:
Academic Press
eBook ISBN:
9780123812735
Print ISBN:
9780123812728

About the serial-volume-editor

John Abelson

Affiliations and Expertise

California Institute of Technology, Division of Biology, Pasadena, U.S.A.