Germfree Research

Germfree Research

Biological Effect of Gnotobiotic Environments

1st Edition - January 1, 1973

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  • Editor: James Heneghan
  • eBook ISBN: 9780323143325

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Germfree Research: Biological Effect of Gnotobiotic Environments is an account of the Proceedings of the Fourth International Symposium on Germfree Research. The volume comprises of 12 sections and 97 chapters. The first two sections serve as introduction to the status of germfree research as an approach to micro-ecology and gnotobiotic technology in clinical medicine. Another aspect discussed in the book deals with nutrition, metabolism, and microbiology. The last section provides a thorough and detailed discussion on gnotobiotic technology. Large part of the book focuses on gnotobiotic environments and its different aspects. Topics discussed include gnotobiotic environment on isolated patients and in dentistry and parasitology, as well as carcinogenesis in and physiological effects of gnotobiotic environments. The book is a very valuable source of information for many students and professionals in the fields of clinical medicine, biology, microbiology, nutritional science, and biochemistry.

Table of Contents

  • Foreword


    Section I. Introduction

    Germfree Research: A New Approach in Micro-Ecology

    Thoughts on the Role of Gnotobiotics in Clinical Medicine

    Section II. Gnotobiotic Technology in Clinical Medicine

    Germfree Technology in Clinical Medicine: Production and Maintenance of Gnotobiotic States in Man

    Patient Isolators Designed in The Netherlands

    A Simplified Plastic Flexible Film Isolator System for the Germfree Delivery and Maintenance of Infants with Immune Deficiency

    Biomedical Applications of Laminar Airflow

    Evaluative Study of Patients with Acute Leukemia under Gnotobiotic Conditions by the Gnotobiotic Project Group of the European Organization for Research on Treatment of Cancer (E.O.R.T.C.)

    A Gauntlet Isolator for Hip Arthroplasty

    Biotyping of Enterobacteriaceae: A Method to Determine the Efficacy of the Barrier Function of Isolation Units

    Section III. Effect of Gnotobiotic Environment on Isolated Patients

    Investigation of Behavior of Leukemia Patients Treated in Germfree Isolators

    Fecal Bile Acids and Neutral Sterols of Gnotobiotic, Antibiotic Fed Normal, and Normal Human Children

    Differences in Absorption of Actively Absorbed Sugar in "Gnotobiotic Patients"

    Use of a Surgical Isolator for Major Surgery: Influence on Wound Contamination and Postoperative Wound Infections

    Clinical Experience with the Control of the Microflora

    Controlled Trial of Prophylaxis of Infections from Exogenous and Endogenous Microorganisms

    Section IV. Carcinogenesis in Gnotobiotic Environments

    Carcinogenesis in Gnotobiotic Rodents

    Spontaneous Tumors in Japan-Born Germfree Rats

    Study on Trans-Species Induction of Reticular Tissue Neoplasms in Germfree CFWW Mice

    Natural Transmission of Leukemia and Mammary Tumors in Conventional and Germfree CFWW Mice

    Chemotherapy of Spontaneous Leukemia in Germfree and Conventional AKR Mice

    Antithrombin Activity in Decontaminated and Conventional Rats with Acute Leukemia

    Cell Transformations in Germfree Fischer Rat

    Biosynthesis of Hepatotoxins in Germfree and Conventional Mice

    The Enhancement of Bone Marrow Colony-Stimulating Factor Produced by Friend Virus (FV-P) Infection in Germfree Mice

    Section V. Gnotobiotic Environments in Dentistry

    The Periodontium of Old Germfree and Conventional Rats

    Periodontal Disease in Gnotobiotic and Conventional Rice Rats

    The Periodontium of Germfree Dogs

    Inflammation after Pulp Exposure of Teeth in Germfree, Defined Flora and Isolator Conventional Mice

    Comparison of Anticaries Effects of Different Polyglucanases in Limited-Flora Hamsters Infected with Streptococcus mutans

    Observations on the Cariogenic Assessment of Streptococci in Gnotobiotic Rats, with Reference to the Taxonomy of Streptococcus mutans

    Relationship of Specific Antibacterial Agglutinins in Saliva to Dental Caries in Gnotobiotic Rats

    Effectiveness of High Speed Dental Drills as Nebulizers and Control of the Generated Aerosols

    Section VI. Nutrition and Metabolism

    The Breakdown of Starch at Different Levels in the Digestive Tract of the Axenic, Gnotoxenic, and Holoxenic Chicken

    Interrelationships between the Gut Microflora and the Response of Chicks to Unidentified Growth Factors

    The Influence of the Antibiotics Aureomycin and Moenomycin (Flavomycin) on Germfree Chicks under Stress Conditions

    Improved Lactation in Germfree Mice Following Changes in the Amino Acid and Fat Components of a Chemically Defined Diet

    Digestibility and Balance Studies of Gnotobiotic Pigs

    The Significance of the Intestinal Microflora on the Growth of Japanese Quail Fed Diets Containing Navy Beans

    Causes and Possible Consequences of Cecal Enlargement in Germfree Rats

    The Bile Acids of the Mouse: Effect of Microflora, Age and Sex

    Biohydrogenation of Long-Chain Fatty Acids by Intestinal Microorganisms

    Urinary Calculi in Germfree Rats: Alleviated by Varying the Dietary Minerals

    Protein Metabolism in the Germfree and Conventional Chick

    Role of Cysteine Ethyl Ester and Indigenous Microflora in the Pathogenesis of an Experimental Hemolytic Anemia, Azotemia, and Pancreatic Acinar Atrophy

    Nitrogen Metabolism in Germfree Rats

    Electron Microscopic Studies on Cardiac Lesions in Germfree and Conventional Rats Fed Diet Deficient in Vitamin B1

    The Role of Urea in the Hyperammonemia of Eck Fistula Dogs

    Section VII. Microbiology: Infectious Diseases and Antibiotics

    Histopathological and Biochemical Responses of Germfree and Conventional Mice with Salmonella Infection

    Studies on the Pathogenesis of Candida albicans by the Use of Germfree Mice

    Development of Kanamycin-Resistant Serratia marcescens in Gnotobiotic Mice

    Erysipelothrix rhusiopathiae Infection in Hyper sensitized Gnotobiotic Pigs and SEM Examination of the Lesions

    The Effect of Antibiotics against the Intestinal Bacterial Flora

    Influence of Nutritional Levels of Spiramycin and Virginiamycin on the Bacterial Metabolites in the Gastrointestinal Tract and Urine of Artificially Reared Early Weaned Piglets

    Study of the Gastrointestinal Microflora of Suckling and Early Weaned Piglets Using Different Feeding Systems and Feed Additives

    Studies with Lactobacillus casei in Gnotobiotic Mice

    Immunologie Conversion of Vibrio cholerae in Gnotobiotic Mice

    Significance of Intestinal Bacterial Flora to Infection

    Experimental Airborne Influenza PR8-A Infections in Germfree Mice

    Pathogenicity of Candida albicans as Influenced by Escherichia coli, Gentamicin Therapy, and Thermal Injury in the Germfree Mouse

    Section VIII. Microbiology: "Normal" Flora

    Equilibrium between Ten Strictly Anaerobic Bacterial Strains in the Digestive Tract of "Gnotoxenic" Mice and Rats. Role of the Diet

    Kinetics of the Establishment of a Strictly Anaerobic Microflora in the Digestive Tract of "Gnotoxenic" Mice between Birth and Weaning

    Patterns of Interaction in Gnotobiotic Mice among Bacteria of a Synthetic "Normal" Intestinal Flora

    Control of Staphylococci in the Gut of Mice

    The Slow-To-Rapid Lactose Fermentation Shift in the Coliform Bacterial Population of the Germfree-Like Ceca of Antibiotic-Treated Rats

    Section IX. Gnotobiotic Environments in Parasitology

    Aspiculuris tetraptera Nietsch, 1821, in Germfree and Conventional Mice

    Comparative Pathology and Lesions of Experimental Infections with Eimeria tenella in Germfree, Specific Pathogen-Free, and Conventional Chickens

    The Development of Eimeria tenella in Germfree Chickens

    Eimeria maxima Infections in Germfree and Gnotobiotic Chickens

    Section X. Radiation and Immunology

    Influence of Antibiotic Decontamination on Thrombocytopenic Bleeding in Irradiated Rats

    Evidence for Graft vs. Host Reaction in the Germfree Allogeneic Radiation Chimera

    Mortality of Secondary Disease in Antibiotic-Treated Mouse Radiation Chimeras

    Influence of a Defined Flora on the Serum Proteins of Gnotobiotic Rats

    Comparisons of Natural and Immune Antibodies to Teichoic Acids in Germfree and Conventional Guinea Pigs

    Viral Enteric Infection and Local IgA Antibody: Studies in Germfree Mice Thymectomized and/or Infected with a Mouse Adenovirus

    Ascorbic Acid and Glucocorticoid Levels in Relation to Underdeveloped Lymphatic Tissue in Germfree Guinea Pigs

    Section XI. Physiological Effects of Gnotobiotic Environments

    Testicular Functions of Germfree Mice

    Studies of Vasopressin in Germfree and Conventional Rats

    Responsiveness of Jejunal-Ileal Mesentery Microvessels in Unoperated and Cecectomized Germfree Rats to Some Smooth Muscle Agonists

    Epinephrine Inhibitory Substance in Intestinal Contents of Germfree Rats

    Hemorrhagic Shock in Cecectomized Germfree Rats

    Cecal Reduction in "Gnotoxenic" Rats

    The Influence of Gastrointestinal Flora on Digestive Utilization of Fatty Acids in Rats

    Intestinal Strangulation in Monocontaminated Rats

    Studies on Enteropathogenic E. coli Enterotoxins; Microbial Flora and Disaccharidase Concentration Changes in Ligated Pig Intestinal Loop

    The Gastrointestinal Tract of Gnotobiotic Pigs

    Effects of Germfree Rodent's Cecal Contents on Spontaneous Villus Movement

    Chronic Mild Diarrhea in Germfree Rodents: A Model Portraying Host-Flora Synergism

    Section XII. Gnotobiotic Technology

    A Lightweight Stainless Steel Isolation System for Adult Gnotobiotic Dogs

    Raising Germfree Baboons (Papio cynocephalus)

    The Establishment of a Defined Hamster Colony Utilizing Gnotobiotic Techniques

    Studies on Rearing the Hamster Germfree

    Raising Specified Pathogen-Free Guinea Pigs and Rabbits by Using Gnotobiotechniques

    Observations on the Reproductive Performance and Laboratory Use of Inbred Mice Whose Ancestors were Derived via Surgical/Isolator Techniques

    Technology Advancements in the Growth of Germfree Plants at the Manned Spacecraft Center

    Plant Gnotobiotic Research in Plant Pathology and Plant Physiology

    Bacterial Growth, Glucidic and Nitrogenous Metabolism in Gnotoxenic Ensilages of Lucerne, Ray Grass, and Fescue

    Studies on the Nutrition of the Snail Biomphalaria glabrata under Axenic Conditions


Product details

  • No. of pages: 694
  • Language: English
  • Copyright: © Academic Press 1973
  • Published: January 1, 1973
  • Imprint: Academic Press
  • eBook ISBN: 9780323143325

About the Editor

James Heneghan

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