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Germfree Life and Gnotobiology focuses on the theory and general aspects of germfree research and gnotobiology. Using a phylogenetic approach, the book provides a summary of germfree work in all phyla, from bacteria and viruses to protozoans and invertebrates. It characterizes germfree vertebrates based on data on morphology, biochemistry, nutrition, serology, and physiology. This book is organized into six chapters and begins with an overview of germfree life and gnotobiology, including biological isolation as the basis of germfree life, germfree conditions in nature, and the biological significance of germfree research. A section is devoted to nomenclature and terminology. The chapters on methods and nutrition are sufficiently detailed to serve as guides for experimental work. The book concludes with a chapter on exploratory research that explores the inoculation of germfree animals with known kinds of microorganisms. Terms have been introduced to describe concepts and provide precise communication. This book is a valuable source of information for scientists and researchers engaged in germfree research as a biological tool.
1 Theory and General Aspects of Germfree Life and Gnotobiology
Biological Isolation as the Basis of Germfree Life
Germfree Conditions in Nature
Essential Elements in Germfree Research
Biological Significance of Germfree Research
Relationship to Other Enclosed Systems
Nomenclature and Terminology
2 Phylogenetic Development of Germfree Research
Germfree Culture of Tissues
3 Germfree Animal Techniques
Obtaining Germfree Animals
Summary of Methods
Production, Maintenance, and Repair Problems
4 Nutrition of Germfree Animals
Intestinal Synthesis Theory
Diet and Microorganisms
Diet Changes during Sterilization
The First Colony of Germfree Mammals
Diets and Feeding
5 Characteristics of Germfree Animals
Systems, Organs, and Tissues
Abnormalities in Form and Function
6 Gnotophoric Animals
III. Diets for Germfree Animals
- No. of pages:
- © Academic Press 1963
- 1st January 1963
- Academic Press
- eBook ISBN:
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