Genetics and Biotechnology of Bacilli - 1st Edition - ISBN: 9780122741609, 9780323138642

Genetics and Biotechnology of Bacilli

1st Edition

Authors: A.T. Ganesan
eBook ISBN: 9780323138642
Imprint: Academic Press
Published Date: 27th November 1984
Page Count: 421
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Genetics and Biotechnology of Bacilli contains the proceedings of the Second International Conference on Genetics and Biotechnology of Bacilli, held at Stanford University in Stanford, California, on July 6-8, 1983. Contributors discuss the progress that has been made concerning the genetics and biotechnology of Bacillus and focus on topics built around the themes of chromosomal organization, secretion, transcription, gene cloning, gene expression, and synthesis of sporulation-associated products. This text is organized into 33 chapters and begins with an overview of bacteriophage lambda biology, with emphasis on lambda insertion, controlled DNA rearrangements, operator-promoter function, and the evolution of extrachromosomal elements. The reader is then introduced to genetic mapping of cloned ribosomal RNA genes, gene amplification in Bacillus subtilis, beta-lactamases of Bacilli, and the role of a Bacillus secretion vector in the secretion of foreign gene products. This book also gives an account of various facets of Bacillus biology, especially in the identification of promoters, cloning of foreign genes, and selection of expressed gene products. This reference material is a valuable resource for geneticists, microbiologists, and biotechnologists, as well as students and researchers in the fields of molecular biology and biochemistry.

Table of Contents

Contributors Preface Opening Session Opening Address: The Integrated Prophage: Implications and Perspectives Opening Remarks: Scatology and Biotechnology Session I: Chromosomal Organization Genetic Mapping of Cloned Ribosomal RNA Genes Transfer RNA Gene Organization in Bacillus subtilis Structure and Function of the Region of the Replication Origin of the Bacillus subtilis Chromosome Mapping and Cloning DNA from the Replication Terminus Region of the Bacillus subtilis Chromosome and the Manner of Replication Fork Approach at Termination Two Types of Binding of pUB110 to Bacillus subtilis Membrane Gene Amplification in Bacillus subtilis: The Establishment of Multiple Tandemly-Repeated Copies of a Heterologous DNA Segment in the Bacterial Chromosome New Genetic Methods, Molecular Cloning Strategies, and Gene Fusion Techniques for Bacillus subtilis Which Take Advantage of Tn917 Insertional Mutagenesis The Sucrose System as a Model of Genetic Regulation in Bacillus subtilis Session II: Secretion ß-Lactamases of Bacilli: Nature and Processing A Genetic Approach to Levansucrase Secretion in Bacillus subtilis Secretion of Foreign Gene Products by the Aid of a Bacillus Secretion Vector Cloning of a Serine Protease Gene from Bacillus amyloliquefaciens and Its Expression in Bacillus subtilis Cloning and Sequencing of a Region Controlling Efficient Expression of Subtilisin from Bacillus amyloliquefaciens Secretion Vector of Bacillus subtilis Constructed from the Bacillus subtilis α-Amylase Promoter and Signal Peptide Coding Region Session III: Transcription In Vitro Transcription of Bacteriophage 029 DNA Structure, Regulation, and Genetic Locus of a Temporally Expressed Promoter of Bacillus subtilis Inhibition by Lipiarmycin of Bacillus subtilis RNA Polymerase Cloning using Bacteriophage SPPlv as the Vector: Vector Development, Stability, and Expression


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Academic Press
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A.T. Ganesan

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