Genetics and Biotechnology of Bacilli

1st Edition - November 27, 1984
Author: A.T. Ganesan
Language: English
eBook ISBN:
9 7 8 - 0 - 3 2 3 - 1 3 8 6 4 - 2

Genetics and Biotechnology of Bacilli contains the proceedings of the Second International Conference on Genetics and Biotechnology of Bacilli, held at Stanford University in… Read more

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Genetics and Biotechnology of Bacilli contains the proceedings of the Second International Conference on Genetics and Biotechnology of Bacilli, held at Stanford University in Stanford, California, on July 6-8, 1983. Contributors discuss the progress that has been made concerning the genetics and biotechnology of Bacillus and focus on topics built around the themes of chromosomal organization, secretion, transcription, gene cloning, gene expression, and synthesis of sporulation-associated products. This text is organized into 33 chapters and begins with an overview of bacteriophage lambda biology, with emphasis on lambda insertion, controlled DNA rearrangements, operator-promoter function, and the evolution of extrachromosomal elements. The reader is then introduced to genetic mapping of cloned ribosomal RNA genes, gene amplification in Bacillus subtilis, beta-lactamases of Bacilli, and the role of a Bacillus secretion vector in the secretion of foreign gene products. This book also gives an account of various facets of Bacillus biology, especially in the identification of promoters, cloning of foreign genes, and selection of expressed gene products. This reference material is a valuable resource for geneticists, microbiologists, and biotechnologists, as well as students and researchers in the fields of molecular biology and biochemistry.

Contributors

Preface

Opening Session

Opening Address: The Integrated Prophage: Implications and Perspectives

Opening Remarks: Scatology and Biotechnology

Session I: Chromosomal Organization

Genetic Mapping of Cloned Ribosomal RNA Genes

Transfer RNA Gene Organization in Bacillus subtilis

Structure and Function of the Region of the Replication Origin of the Bacillus subtilis Chromosome

Mapping and Cloning DNA from the Replication Terminus Region of the Bacillus subtilis Chromosome and the Manner of Replication Fork Approach at Termination

Two Types of Binding of pUB110 to Bacillus subtilis Membrane

Gene Amplification in Bacillus subtilis: The Establishment of Multiple Tandemly-Repeated Copies of a Heterologous DNA Segment in the Bacterial Chromosome

New Genetic Methods, Molecular Cloning Strategies, and Gene Fusion Techniques for Bacillus subtilis Which Take Advantage of Tn917 Insertional Mutagenesis

The Sucrose System as a Model of Genetic Regulation in Bacillus subtilis

Session II: Secretion

ß-Lactamases of Bacilli: Nature and Processing

A Genetic Approach to Levansucrase Secretion in Bacillus subtilis

Secretion of Foreign Gene Products by the Aid of a Bacillus Secretion Vector

Cloning of a Serine Protease Gene from Bacillus amyloliquefaciens and Its Expression in Bacillus subtilis

Cloning and Sequencing of a Region Controlling Efficient Expression of Subtilisin from Bacillus amyloliquefaciens

Secretion Vector of Bacillus subtilis Constructed from the Bacillus subtilis α-Amylase Promoter and Signal Peptide Coding Region

Session III: Transcription

In Vitro Transcription of Bacteriophage 029 DNA

Structure, Regulation, and Genetic Locus of a Temporally Expressed Promoter of Bacillus subtilis

Inhibition by Lipiarmycin of Bacillus subtilis RNA Polymerase

Cloning using Bacteriophage SPPlv as the Vector: Vector Development, Stability, and Expression

Session IV: Cloning

Development of an Inducible Promoter for Controlled Gene Expression in Bacillus subtilis

Cloning and Characterization of the Gene for a Thiol-Activated Cytolysin in Bacillus subtilis

Chloramphenicol Inducibility of Foreign Gene Expression in Bacillus subtilis

Parameters Affecting the Stability of Recombinant Bifunctional Plasmid Transfer from Escherichia coli to Bacillus subtilis

Cloning of Genetic Material in Bacilli

Chromogenic Detection of Genetic Regulatory Signals Cloned in Bacillus subtilis

Session V: Synthesis of Sporulation Associated Products

Cloning and Sequence Analysis of the spoOA Locus of Bacillus subtilis

Amplification of Sporulation Genes and Its Effect on Differentiation

Genetic and Biochemical Study of Bacillus thuringiensis

Regulation of Bacillus thuringiensis Protoxin Production

Structural and Regulatory Analysis of a Cloned Bacillus thuringiensis Crystal Protein Gene

Plasmid-Associated Delta-Endotoxin Production in Bacillus thuringiensis

Focus Sessions

Phage

Spores

General Genetics

Index