Genetics and Biotechnology of Bacilli, Volume 2
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Genetics and Biotechnology of Bacilli, Volume 2 is a collection of papers from the "Fourth International Conference on Bacilli" held in California on June 21-24, 1987. One paper reviews the results of cloning and characterization of genes for secreted enzyme and of genes that control the expression of secreted enzymes in relation with other prokaryotic regulatory systems. Other papers tackle the regulation of gene expression during sporulation, the sigma factors, bacterial toxins, and antibiotic resistance genes. One paper reports that three genes responsible for the code for peptides found in BT cuboidal crystals have been successfully cloned. The three codes have different toxic characteristics in relation to tobacco hornworm larvae or mosquito larvae. Other papers examine replication. Such examination pertains to the two levels of control on the chromosome involved in DNA replication, or to the possible functional importance that several membranes associated DNA subcomplexes can have in Bacillus subtilis, where one of these appear to control initiation. Other papers discus secretion and extracellular enzymes, as well as, the different genetic systems and methods. This collection can prove beneficial for biochemists, micro-biologists, cellular researchers, and academicians involved in the study of cellular biology, microchemistry, or toxicology.
Table of Contents
Preface
Gene Expression
Upstream Activating Sequences in Bacillus Subtilis
Levansucrase Induction by Sucrose in Bacillus Subtilis Involves an Antiterminator. Homology with the Escherichia Coli bgl Operon
Inducible Expression System Based on Sucrose Metabolism Genes of Bacillus Subtilis
Development of an Expression and Secretion System in Bacillus Subtilis Utilizing sacQ
Polypeptides Activating the Synthesis of Secreted Enzymes
Enhancement of mRNA Level by prtR
Regulation of Post Exponential Gene Expression: Transcriptional Control of the Subtilisin Gene
Isolation and Characterization of a Novel Bacillus Subtilis and Bacillus Natto Gene That Enhances Protease Production
Characterization of the Bacillus Subtilis pur Operon: New Insights Into Gene Regulation
Purine Gene Organization and Regulation in Bacillus Subtilis
Mutations in the Spacer Region of a Bacillus Subtilis Promoter
Control of Transcription and Translation of the Subunits of Aspartokinase II from Bacillus Subtilis
Cloning and Expression of a Chemotaxis Gene in Bacillus Subtilis
Analysis of the Bacillus Subtilis Xylose Regulon
Transcription of the Bacillus Subtilis α-Amylase Gene
Genetic Regulation of Cytochrome aa3 in Bacillus Subtilis
Isolation and Characterization of citC Gene of Bacillus Subtilis
The Role of Ribosomal Protein S1 in Species-Specific Protein Synthesis
Dual Regulation of the RecE Gene
Chromosomal Organization and Spontaneous Deletions of rrn Operons in Bacillus Subtilis
Regulation of Gene Expression During Sporulation
Influence of abrB on the Transcription of the Sporulation-Associated Genes spoVG and spoOH in Bacillus Subtilis
Molecular Cloning of the Transcription Inhibitor AbrB of Bacillus Subtilis
Analysis of Glucose Sensitive Expression of amyF and spoOA Genes in Bacillus Subtilis
Manipulation of Gene Copy Number in Bacillus Subtilis Using Integrative Plasmids
Regulatory Interactions Between the Spo Genes
Structure and Regulation of a Bacillus Subtilis Gene That Is Transcribed by the ΕσΒ Form of RNA Polymerase Holoenzyme
Regulation of Expression of the SSPE Gene Which Codes for Small, Acid Soluble Protein-Gamma of Bacillus Subtilis Spores
Acid Soluble Spore Proteins from Clostridium Perfringens
The Bacillus Subtilis outB Gene Is Autogenously Regulated
Role of Extracellular Factors in the Control of Sporulation in Bacillus Subtilis
Sigma Factors
Use of λgtll and Antibody Probes to Isolates Genes Encoding RNA Polymerase Subunits from Bacillus Subtilis
Cloning and Characterization of the Bacillus Subtilis Sigma-28 Gene
Transcription of the SPOIIG Operon
Transcription of Two Early Bacteriophage φ29 Promoters by Bacillus Subtilis RNA Polymerase (Sigma 43)
A Novel Bacillus Subtilis Transcriptional Activity Is Associated with RNA Polymerase Isolated from Cells at a Late Stage in Sporulation
Characterization of the Promoters for Two UV-Inducible Transcriptional Units Carried by Plasmid pIP404 from Clostridium Perfringens
Transcription Initiation and Termination Signals of the Bacillus Subtilis Phage φ29 DNA
Bacterial Toxins and Antibiotic Resistance Genes
Cloning of a Family of Genes Which Includes the Larvicidal Toxin of Bacillus Sphaericus
Bacillus Thuringiensis Var. San Diego: Properties and Sequence of a Coleopteran Active Delta Endotoxin
Characterization of Bacillus Thuringiensis Isolates Effective on Resistant Indian Meal Moth
Genes Encoding Peptides of the Small Cuboidal Crystals of Bacillus Thuringiensis
Translational Attenuation Control of Gene Expression in Streptomyces
Homology of the Deduced Protein Structures of the Plasmid-Encoded Tetracycline-Resistance Determinants in Gram-Positive Bacteria
Ribosome Stalling in the Cat-86 Regulatory Leader Induces Gene Expression
Regulation of an Inducible Staphylococcus Aureus Cat Gene by TYanslational Attenuation
Replication
The dnaB Operon of Bacillus Subtilis: Anchorage-Anchor Model for the Chromosome Replication-Initiation, Partition, and Membrane-Attachment of oriC Area
DnaA-boxes in the OriC Region of the Bacillus Subtilis Chromosome Are Involved in Regulation of Initiation of Chromosomal Replication
Studies on In Vitro Synthesis of Bacillus Subtilis DNA by a Purified Membrane Associated DNA Complex and Binding of Membrance Proteins to Unique Origin Fragments
Regulation of Chromosomal Initiation in Bacillus Subtilis
Molecular Structure of the Replication Origin of a Bacillus Amyloliquefaciens Plasmid pFTB14
Novel Integrational Vectors for Bacillus Subtilis Based on Coliphage M13 and Their Use for the Analysis of Regulated Promoters
Segregational Plasmid Instability in Bacillus Subtilis
The Processing of tRNA Precursors by the Catalytic RNA Component of RNASE Ρ
Identification of a Holoenzyme Form of DNA Polymerase III in Bacillus Subtilis
Secretion and Extracellular Enzymes
In Vitro Derived Phosphatase Mutants of Bacillus
Stability and Expression in Bacillus Subtilis of an α-Amylase Hybrid Plasmid
Expression of Integrated Bacillus Amyloliquefaciens α-Amylase Gene in the Genome of Bacillus Subtilis
Structure of Bacillus Pumilus Xylanase
Similarity in the Primary Structures of Amylases and Cyclodextrin Glucanotransferase of Bacilli
Regulation of ß-Lactamase Synthesis in Bacillus Licheniformis
Regulation of the PEN Genes in Bacillus Licheniformis
Construction of an Efficient Secretion Host—Vector System in Bacillus Subtilis
Expression and Secretion of Human Interleukin-1 in Bacillus Subtilis
Human Growth Hormone-Nuclease Fusion Proteins: Bacillus Subtilis Mutants with Altered Growth Hormone Production and Secretion
Molecular Cloning and Nucleotide Sequence of a Gene Involved in the Production of Extracellular DNASE by Corynebacterium Glutamicum
New Genetic Systems
Conjugal Gene Transfer in Clostridium Acetobutylicum
Transformation of Lactobacillus Plantarum by Plasmid DNA
Development of Genetic Methods in Bacillus Megaterium
Construction of Plasmid Vector Systems for Clostridium Acetobutylicum
Index
Product details
- No. of pages: 429
- Language: English
- Copyright: © Academic Press 1988
- Published: July 28, 1988
- Imprint: Academic Press
- eBook ISBN: 9780323138710
About the Author
A.T. Ganesan
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