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Genetic Engineering Techniques: Recent Developments - 1st Edition - ISBN: 9780123582508, 9780323153577

Genetic Engineering Techniques: Recent Developments

1st Edition

Editor: P.C. Huang
eBook ISBN: 9780323153577
Imprint: Academic Press
Published Date: 1st January 1982
Page Count: 374
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Genetic Engineering Techniques: Recent Developments covers the proceedings of the 1982 Genetic Engineering Techniques symposium held in Taipei. The book is organized into 21 chapters that discuss the application of recombination DNA methods in the study of DNA structure and DNA-protein interactions; the use of chemically synthesized genes in cloning; and gene expression. After briefly presenting the major strategies underlying genetic engineering technology and rapid method for sequencing DNA, the book examines the reaction mechanism of a multifunctional Type I enzyme and the organization and expression of the human adenovirus. The second section describes several approaches in analyzing transcriptional processes in prokaryotic and eukaryotic systems. This section also deals with cloning vectors and procedures of cDNA. The subsequent section describes a molecular approach to functional analysis of the influenza virus surface hemagglutinin; the transposition specificity for the transposons 3 and 4 elements; and the biological properties of human T-cell growth factor gene. The fourth section discusses the principles of hybridoma technology and its numerous applications to biological research. The remaining chapters of the book present laboratory courses designed to familiarize researchers with the principles and basic procedures in biological experiments. Genetic engineering researchers, agriculturists, and geneticists will find this book invaluable.

Table of Contents



I. The Basic Tools

Genetic Engineering Technology—An Overview and Some Recent Advances

The Methylated State of a DNA Sequence and Its Effect on the Functions of the Restriction Endonuclease from E. coli K

The Application of Recombinant DNA Methods to Structural Studies of DNA

Organization and Expression of the Human Adenovirus Genes Encoding the DNA Binding Protein and the DNA Terminal Protein

II. Cloning

Some Approaches for Analyzing Transcriptional Processes in Prokaryotic and Eukaryotic Systems

Cloning of Double Stranded cDNA form Major and Minor Components of mRNA

Nucleotide Sequence, DNA Modification, and In Vitro Transcription of Alu Family Repeats in the Human α-Like Globin Gene Cluster

Study on the Primary Structure of the Isoleucyl-tRNA Synthetase from Escherichia coli MRE 600

III. Expression

Expression of the Cloned p21 Transforming ras Gene of Harvey Murine Sarcoma Virus

Influenza A Viruses as Studied by Recombinant DNA Techniques

Specificity of Tn3 and Tn4 Transposition

Human T-Cell Growth Factor (TCGF): Biological Properties and Isolation of Biologically Active mRNA

IV. Potentials

Expression of Eukaryotic Genes in Bacillus subtilis

Expression of Hepatitis Surface Antigen in Mammalian Cells

Monoclonal Antibodies: Preparation by Hybridoma Method and Applications in Biological Research

V. Summary

New Vistas of Genetics—1982

VI. Workshop

Production of Monoclonal Antibodies by Hybridoma Method

Electron Microscopy

Isolation of Recombinant Clones from Genomic DNA Library by In Situ Hybridization

Plasmid Transformation and Preparation

The Purification of Restriction Enzymes and DNA Substrates. Their Use in DNA Sequencing



No. of pages:
© Academic Press 1982
1st January 1982
Academic Press
eBook ISBN:

About the Editor

P.C. Huang

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