Genetic Engineering Techniques: Recent Developments

Genetic Engineering Techniques: Recent Developments

1st Edition - January 1, 1982

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  • Editor: P.C. Huang
  • eBook ISBN: 9780323153577

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Description

Genetic Engineering Techniques: Recent Developments covers the proceedings of the 1982 Genetic Engineering Techniques symposium held in Taipei. The book is organized into 21 chapters that discuss the application of recombination DNA methods in the study of DNA structure and DNA-protein interactions; the use of chemically synthesized genes in cloning; and gene expression. After briefly presenting the major strategies underlying genetic engineering technology and rapid method for sequencing DNA, the book examines the reaction mechanism of a multifunctional Type I enzyme and the organization and expression of the human adenovirus. The second section describes several approaches in analyzing transcriptional processes in prokaryotic and eukaryotic systems. This section also deals with cloning vectors and procedures of cDNA. The subsequent section describes a molecular approach to functional analysis of the influenza virus surface hemagglutinin; the transposition specificity for the transposons 3 and 4 elements; and the biological properties of human T-cell growth factor gene. The fourth section discusses the principles of hybridoma technology and its numerous applications to biological research. The remaining chapters of the book present laboratory courses designed to familiarize researchers with the principles and basic procedures in biological experiments. Genetic engineering researchers, agriculturists, and geneticists will find this book invaluable.

Table of Contents


  • Contributors

    Preface

    I. The Basic Tools

    Genetic Engineering Technology—An Overview and Some Recent Advances

    The Methylated State of a DNA Sequence and Its Effect on the Functions of the Restriction Endonuclease from E. coli K

    The Application of Recombinant DNA Methods to Structural Studies of DNA

    Organization and Expression of the Human Adenovirus Genes Encoding the DNA Binding Protein and the DNA Terminal Protein

    II. Cloning

    Some Approaches for Analyzing Transcriptional Processes in Prokaryotic and Eukaryotic Systems

    Cloning of Double Stranded cDNA form Major and Minor Components of mRNA

    Nucleotide Sequence, DNA Modification, and In Vitro Transcription of Alu Family Repeats in the Human α-Like Globin Gene Cluster

    Study on the Primary Structure of the Isoleucyl-tRNA Synthetase from Escherichia coli MRE 600

    III. Expression

    Expression of the Cloned p21 Transforming ras Gene of Harvey Murine Sarcoma Virus

    Influenza A Viruses as Studied by Recombinant DNA Techniques

    Specificity of Tn3 and Tn4 Transposition

    Human T-Cell Growth Factor (TCGF): Biological Properties and Isolation of Biologically Active mRNA

    IV. Potentials

    Expression of Eukaryotic Genes in Bacillus subtilis

    Expression of Hepatitis Surface Antigen in Mammalian Cells

    Monoclonal Antibodies: Preparation by Hybridoma Method and Applications in Biological Research

    V. Summary

    New Vistas of Genetics—1982

    VI. Workshop

    Production of Monoclonal Antibodies by Hybridoma Method

    Electron Microscopy

    Isolation of Recombinant Clones from Genomic DNA Library by In Situ Hybridization

    Plasmid Transformation and Preparation

    The Purification of Restriction Enzymes and DNA Substrates. Their Use in DNA Sequencing

    Index


Product details

  • No. of pages: 374
  • Language: English
  • Copyright: © Academic Press 1982
  • Published: January 1, 1982
  • Imprint: Academic Press
  • eBook ISBN: 9780323153577

About the Editor

P.C. Huang

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