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Genetic Engineering Techniques: Recent Developments covers the proceedings of the 1982 Genetic Engineering Techniques symposium held in Taipei. The book is organized into 21 chapters that discuss the application of recombination DNA methods in the study of DNA structure and DNA-protein interactions; the use of chemically synthesized genes in cloning; and gene expression. After briefly presenting the major strategies underlying genetic engineering technology and rapid method for sequencing DNA, the book examines the reaction mechanism of a multifunctional Type I enzyme and the organization and expression of the human adenovirus. The second section describes several approaches in analyzing transcriptional processes in prokaryotic and eukaryotic systems. This section also deals with cloning vectors and procedures of cDNA. The subsequent section describes a molecular approach to functional analysis of the influenza virus surface hemagglutinin; the transposition specificity for the transposons 3 and 4 elements; and the biological properties of human T-cell growth factor gene. The fourth section discusses the principles of hybridoma technology and its numerous applications to biological research. The remaining chapters of the book present laboratory courses designed to familiarize researchers with the principles and basic procedures in biological experiments. Genetic engineering researchers, agriculturists, and geneticists will find this book invaluable.
I. The Basic Tools
Genetic Engineering Technology—An Overview and Some Recent Advances
The Methylated State of a DNA Sequence and Its Effect on the Functions of the Restriction Endonuclease from E. coli K
The Application of Recombinant DNA Methods to Structural Studies of DNA
Organization and Expression of the Human Adenovirus Genes Encoding the DNA Binding Protein and the DNA Terminal Protein
Some Approaches for Analyzing Transcriptional Processes in Prokaryotic and Eukaryotic Systems
Cloning of Double Stranded cDNA form Major and Minor Components of mRNA
Nucleotide Sequence, DNA Modification, and In Vitro Transcription of Alu Family Repeats in the Human α-Like Globin Gene Cluster
Study on the Primary Structure of the Isoleucyl-tRNA Synthetase from Escherichia coli MRE 600
Expression of the Cloned p21 Transforming ras Gene of Harvey Murine Sarcoma Virus
Influenza A Viruses as Studied by Recombinant DNA Techniques
Specificity of Tn3 and Tn4 Transposition
Human T-Cell Growth Factor (TCGF): Biological Properties and Isolation of Biologically Active mRNA
Expression of Eukaryotic Genes in Bacillus subtilis
Expression of Hepatitis Surface Antigen in Mammalian Cells
Monoclonal Antibodies: Preparation by Hybridoma Method and Applications in Biological Research
New Vistas of Genetics—1982
Production of Monoclonal Antibodies by Hybridoma Method
Isolation of Recombinant Clones from Genomic DNA Library by In Situ Hybridization
Plasmid Transformation and Preparation
The Purification of Restriction Enzymes and DNA Substrates. Their Use in DNA Sequencing
- No. of pages:
- © Academic Press 1982
- 1st January 1982
- Academic Press
- eBook ISBN:
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