General Methods
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Methods in Carbohydrate Chemistry, Volume VIII: General Methods describes the principles and mode of operation of general methods for the analysis and structural characterization of carbohydrates. This book is organized into two sections encompassing 50 chapters. The first section highlights the methods on automated chromatographic techniques; enzymic and other methods for structural analysis of polysaccharides. This section also explores the application of 13C NMR spectroscopy to carbohydrate chemistry. The second section describes the synthesis of deoxy and branched-chain sugars, 1,2-transglycosides, de-N- and de-O-sulfation, and de-N-acetylation of polysaccharides. This book is an invaluable source for organic and analytical chemists, as well as for carbohydrate scientists and researchers.
Table of Contents
Contributors to Volume VIII
Preface
Outline of Volumes I-VII
Errata and Additions—Volumes I-VII
Section I. General Methods of Separation and Analysis
Chromatography
1. Fully Automatic Ion-Exchange Chromatographie Analysis of Neutral Monosaccharides and Oligosaccharides
2. Fully Automatic Gel-Permeation Chromatographie Analysis of Neutral Oligosaccharides
3. Separation and Automated Analysis of Phosphorylated Intermediates
4. High-Performance Liquid Chromatography
5. Gel Permeation Chromatography
6.Analysis and Structural Characterization of Amino Sugars by Gas-Liquid Chromatography and Mass Spectrometry
Analysis of Polysaccharides by Chemical, Physical, and Enzymic Methods
7. Sequential Degradation of Methylated Polysaccharides
8. Enzymic Method for Determination of Glycerol Chain Lengths of Hexans
9. Enzymic Method for Determination of the Degree of Polymerization of Glucans and Xylans
10. Enzymic Analysis of Acidic Glycosaminoglycans
11. Constituent Analysis of Glycosaminoglycans
12. Determination of Polysaccharide Structures with 13C NMR
13. Determination of the Sugar Sequence and Glycosidic Bond Arrangement of Heteroglycans by an Integrated Analytical Scheme
14. Oxidation of Acetylated Carbohydrates with Chromium Trioxide in Acetic Acid
15. Specific Cleavage of Polysaccharides Containing Uronic Acid Residues by the Hoffman-Veerman Reaction
16. Determination of Carboxymethyl Ether Groups in Sodium O-Carboxymethylcellulose(CMC)
Other Chemical, Physical, and Enzymic Methods
17. Enzymic Determination of D-Galactose
18. Enzymic Determination of D-Glucose, D-Fructose, and D-Mannose
19. Microenzymic Procedures for the Identification of Carbohydrates
20. Method for Measuring the Affinity between Carbohydrate Ligands and Certain Proteins
21. 13C NMR Spectroscopy of Isotopically Enriched Carbohydrates
Section II. Preparation of Mono-, Oligo-, and Polysaccharides and Their Derivatives
Monosaccharides and Their Derivatives
22. Methyl α-D-Altropyranoside and Its 4,6- and 3,4-O-Isopropylidene Derivatives
23. L-Gulose and Methyl ß-L-Gulofuranoside
24. L-Idose and L-Iduronic Acid
25. Applications of the 1,3-Dithiane Procedure for the Synthesis of Branched-Chain Carbohydrates L-Streptose, Methyl ß-D-Hamameloxide, Methyl 2,3,6-Trideoxy-2-C-(2-Benzoxyacetyl)-2,3,6-trideoxy-α-L-threo-hexopyranoside
26. 2,6-Dideoxy-D-ribo-hexose (Digitoxose)
27. 2,6-Dideoxy-α-L-lyxo-hexose (2-Deoxy-α-L-fucose)
28. Methoxymercuration in Synthetic Carbohydrate Chemistry Methyl 2,6-Dideoxy-α-L-arabino-hexopyranoside
Polysaccharides
29. Isolation and Purification of Carbohydrate Antigens
Unsaturated Sugars
30. 3-Deoxyglycals (1,5-Anhydro-1,2,3-trideoxyhex-1-enitols) from Hex-2-enopyranosides
Deoxyhalo Sugars
31. Preferential Halogenation of the Primary Hydroxyl Group
Glycosides and Glycosylamines
32. Complexes of Carbohydrates with Metal Cations Methyl ß-D-Mannofuranoside
33. 1,2-Orthoester Formation and Glycosidation with Amide Acetals
34. Preparation of 1,2-trans-Glycosides in the Presence of Stannic Chloride
35. Preparation of 1,2-trans-Glycosides in the Presence of Silver Trifluoromethanesulfonate
36. 1,2-trans-1-Thioglycosides
37. Primary Glycopyranosylamines
38. Production of Sugar Nucleotides by Fermentation
Esters and Amides
39. O-Chloroacetate Derivatives of Sugars as Synthetic Intermediates
40. Cyclic Thionocarbonates
41. Desulfation of Polysaccharides
42. Desulfation of Glycosaminoglycuronan Sulfates
43. De-N-sulfation
44. N-Deacetylation of Polysaccharides
45. N-Trifluoroacetylation Methyl 6-Deoxy-6-trifluoracetamido-α-D-glucopyranoside and Its N-Detrifluoroacetylation
46. Nucleophilic Reaction of the Tosyl Group in Enolic Sugar Derivatives
Ethers
47. Allyl Ethers as Protecting Groups Synthesis of 3-0-[6-O-(α-D-Galactopyranosyl)-/ß-D-galactopyranosyl]-1,2-di-O-stearoyl-L-glycerol, a "Digalactosyl Diglyceride"
Cyclic Acetals
48. Methyl 4,6-O-Benzylidene-α- and -ß-O-glucopyranosides via Acetal Exchange
49. Benzylidenation of Diols with α,α-Dihalotoluenes in Pyridine
Oxidized Products
50. Photochemical Oxidation of Carbohydrates 1,2,3,4-Di-O-isopropylidene-α-D-galacto-hexodialdo-1,5-pyranose
Glossary
Author Index
Subject Index
Product details
- No. of pages: 372
- Language: English
- Copyright: © Academic Press 1980
- Published: June 28, 1980
- Imprint: Academic Press
- eBook ISBN: 9780323153645