General Methods

General Methods

1st Edition - June 28, 1980

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  • Editor: Roy Whistler
  • eBook ISBN: 9780323153645

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Description

Methods in Carbohydrate Chemistry, Volume VIII: General Methods describes the principles and mode of operation of general methods for the analysis and structural characterization of carbohydrates. This book is organized into two sections encompassing 50 chapters. The first section highlights the methods on automated chromatographic techniques; enzymic and other methods for structural analysis of polysaccharides. This section also explores the application of 13C NMR spectroscopy to carbohydrate chemistry. The second section describes the synthesis of deoxy and branched-chain sugars, 1,2-transglycosides, de-N- and de-O-sulfation, and de-N-acetylation of polysaccharides. This book is an invaluable source for organic and analytical chemists, as well as for carbohydrate scientists and researchers.

Table of Contents


  • Contributors to Volume VIII

    Preface

    Outline of Volumes I-VII

    Errata and Additions—Volumes I-VII

    Section I. General Methods of Separation and Analysis

    Chromatography

    1. Fully Automatic Ion-Exchange Chromatographie Analysis of Neutral Monosaccharides and Oligosaccharides

    2. Fully Automatic Gel-Permeation Chromatographie Analysis of Neutral Oligosaccharides

    3. Separation and Automated Analysis of Phosphorylated Intermediates

    4. High-Performance Liquid Chromatography

    5. Gel Permeation Chromatography

    6.Analysis and Structural Characterization of Amino Sugars by Gas-Liquid Chromatography and Mass Spectrometry

    Analysis of Polysaccharides by Chemical, Physical, and Enzymic Methods

    7. Sequential Degradation of Methylated Polysaccharides

    8. Enzymic Method for Determination of Glycerol Chain Lengths of Hexans

    9. Enzymic Method for Determination of the Degree of Polymerization of Glucans and Xylans

    10. Enzymic Analysis of Acidic Glycosaminoglycans

    11. Constituent Analysis of Glycosaminoglycans

    12. Determination of Polysaccharide Structures with 13C NMR

    13. Determination of the Sugar Sequence and Glycosidic Bond Arrangement of Heteroglycans by an Integrated Analytical Scheme

    14. Oxidation of Acetylated Carbohydrates with Chromium Trioxide in Acetic Acid

    15. Specific Cleavage of Polysaccharides Containing Uronic Acid Residues by the Hoffman-Veerman Reaction

    16. Determination of Carboxymethyl Ether Groups in Sodium O-Carboxymethylcellulose(CMC)

    Other Chemical, Physical, and Enzymic Methods

    17. Enzymic Determination of D-Galactose

    18. Enzymic Determination of D-Glucose, D-Fructose, and D-Mannose

    19. Microenzymic Procedures for the Identification of Carbohydrates

    20. Method for Measuring the Affinity between Carbohydrate Ligands and Certain Proteins

    21. 13C NMR Spectroscopy of Isotopically Enriched Carbohydrates

    Section II. Preparation of Mono-, Oligo-, and Polysaccharides and Their Derivatives

    Monosaccharides and Their Derivatives

    22. Methyl α-D-Altropyranoside and Its 4,6- and 3,4-O-Isopropylidene Derivatives

    23. L-Gulose and Methyl ß-L-Gulofuranoside

    24. L-Idose and L-Iduronic Acid

    25. Applications of the 1,3-Dithiane Procedure for the Synthesis of Branched-Chain Carbohydrates L-Streptose, Methyl ß-D-Hamameloxide, Methyl 2,3,6-Trideoxy-2-C-(2-Benzoxyacetyl)-2,3,6-trideoxy-α-L-threo-hexopyranoside

    26. 2,6-Dideoxy-D-ribo-hexose (Digitoxose)

    27. 2,6-Dideoxy-α-L-lyxo-hexose (2-Deoxy-α-L-fucose)

    28. Methoxymercuration in Synthetic Carbohydrate Chemistry Methyl 2,6-Dideoxy-α-L-arabino-hexopyranoside

    Polysaccharides

    29. Isolation and Purification of Carbohydrate Antigens

    Unsaturated Sugars

    30. 3-Deoxyglycals (1,5-Anhydro-1,2,3-trideoxyhex-1-enitols) from Hex-2-enopyranosides

    Deoxyhalo Sugars

    31. Preferential Halogenation of the Primary Hydroxyl Group

    Glycosides and Glycosylamines

    32. Complexes of Carbohydrates with Metal Cations Methyl ß-D-Mannofuranoside

    33. 1,2-Orthoester Formation and Glycosidation with Amide Acetals

    34. Preparation of 1,2-trans-Glycosides in the Presence of Stannic Chloride

    35. Preparation of 1,2-trans-Glycosides in the Presence of Silver Trifluoromethanesulfonate

    36. 1,2-trans-1-Thioglycosides

    37. Primary Glycopyranosylamines

    38. Production of Sugar Nucleotides by Fermentation

    Esters and Amides

    39. O-Chloroacetate Derivatives of Sugars as Synthetic Intermediates

    40. Cyclic Thionocarbonates

    41. Desulfation of Polysaccharides

    42. Desulfation of Glycosaminoglycuronan Sulfates

    43. De-N-sulfation

    44. N-Deacetylation of Polysaccharides

    45. N-Trifluoroacetylation Methyl 6-Deoxy-6-trifluoracetamido-α-D-glucopyranoside and Its N-Detrifluoroacetylation

    46. Nucleophilic Reaction of the Tosyl Group in Enolic Sugar Derivatives

    Ethers

    47. Allyl Ethers as Protecting Groups Synthesis of 3-0-[6-O-(α-D-Galactopyranosyl)-/ß-D-galactopyranosyl]-1,2-di-O-stearoyl-L-glycerol, a "Digalactosyl Diglyceride"

    Cyclic Acetals

    48. Methyl 4,6-O-Benzylidene-α- and -ß-O-glucopyranosides via Acetal Exchange

    49. Benzylidenation of Diols with α,α-Dihalotoluenes in Pyridine

    Oxidized Products

    50. Photochemical Oxidation of Carbohydrates 1,2,3,4-Di-O-isopropylidene-α-D-galacto-hexodialdo-1,5-pyranose

    Glossary

    Author Index

    Subject Index


Product details

  • No. of pages: 372
  • Language: English
  • Copyright: © Academic Press 1980
  • Published: June 28, 1980
  • Imprint: Academic Press
  • eBook ISBN: 9780323153645

About the Editor

Roy Whistler

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