
General methods, glycosaminoglycans, and glycoproteins
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Methods in Carbohydrate Chemistry, Volume VII: General Methods, Glycosaminoglycans, and Glycoproteins is a compilation of useful methods from the entire field of carbohydrate chemistry. This volume puts emphasis on methods and procedures used with glycoproteins and glycosaminoglycans. The book contains chapters devoted to the presentation of methods and procedures used with mono- and disaccharides, glycosides and nucleosides, and amino sugars. The methods for the isolation, purification, and analysis of glycoproteins and glycosaminoglycans are elaborated in detail as well. This volume is intended for the use of chemists, biochemists, and researchers in the field of carbohydrate chemistry.
Table of Contents
Contributors to Volume VII
Preface
Outline of Volumes I-VI
Errata and Additions—Volumes I-VI
Section I. Mono- and Disaccharides
Preparation of Mono- and Disaccharides
1. D-Allose
Glycosides and Nucleosides
2. Methyl α- and β-D-Glucoseptanosides
3. Methyl α-Maltoside
4. Preparation of 2′-Deoxyribonucleosides via Nucleoside Deoxyribosyl Transferase
Amino Sugars
5. 2-Acetamido-2-Deoxy-D-Allose and 2-Acetamido-2-Deoxy-D-altrose
6. 2-Acetamido-2-Deoxy-D-Aulose and 2-Acetamido-2-Deoxy-D-Idose
7. 2-Acetamido-2-Deoxy-D-Galactose and 2-Acetamido-2-Deoxy-D-Talose
Thio Sugars
8. Thio Sugars from Oxidatively Coupled Xanthates
9. Thio Sugars from Cyclic Thionocarbonates
Oxidation Products
10. 3-Deoxy-D-manno-Octulosonic Acid
Deoxyhalo Sugars
11. Direct Replacement of Primary Hydroxyl Groups by Halogen
Esters
12. 1-O-Acetyl-2,3,5-Tri-O-Benzoyl-D-Arabino-Furanose and its Use in Glycosylation by Fusion
Ethers and Anhydrosugars
13. Triphenylmethylation with Tritylpyridinium Flouroborate
14. 2,5-Anhydro-D-Mannitol
Analysis
15. Radiochromatographic Analysis of Reducing Carbohydrates by Radiochromatography
16. Determination of Mono- and Disaccharides in Plant Materials
17. Determination of Anomeric Configuration by nmr
Section II. Oligo- and Polysaccharides
Isolation and Purification of Glycosaminoglycans
18. Heparitin (Heparan) Sulfate Isolation from Beef Lung
Physical Methods of Analysis
19. Analysis and Characterization of Glycosaminoglycans by Nuclear Magnetic Resonance Spectroscopy
20. Microanalysis and Characterization of Glycosaminoglycans from Human Tissue via Zone Electrophoresis
21. Determination of the Molecular Weight of Connective Tissue Glycosaminoglycans (Acid Mucopolysaccharides) by Gel Electrophoresis
22. Circular Dichroism and Optical Rotatory Dispersion in Polysaccharide Structural Analysis: Intrinsic and Extrinsic Cotton Effects
Chemical Methods of Analysis
23. Nitrous Acid Depolymerization of Glycosaminoglycans
24. Specific Degradation of Polysaccharides Containing Uronic Acid Residues
25. Stoichiometric Reduction of Uronic Acid Carboxyl Groups in Polysaccharides
26. Distribution of D-Galactopyranosyl Units in Galactomannans
27. Acyl Localization via Methyl Replacement
Section III. Glycoproteins
Isolation and Purification
28. Isolation of Fetuin
29. Ribonuclease B
30. Ovalbumin of Chicken Egg White
31. Stem Bromelain
Analysis of Glycoproteins
32. Isolation of Glycopeptides from Glycoproteins by Proteolytic Digestion
33. Isolation of Glycopeptides from Cell Membrane Glycoproteins
34. Acid-Catalyzed Hydrolysis and Methanolysis of Glycoproteins
35. Determination of O-Glycosidic Linkages to L-Serine and L-Threonine Residues of Glycoproteins
36. Determination of the 5-O-β-D-Galactopyranosylhydroxy-L-Lysine Linkage in Glycoproteins
37. Determination of the 4-N-2-Acetamido-2-Deoxy-β-D-Glucopyranosyl-L-Asparagine Linkage in Glycoproteins
38. Enzymic Methods for the Structure Analysis of the Carbohydrate Chain of Glycoproteins
39. Removal of Sialic Acids from Glycoproteins by Chemical Methods and Determination of Sialic Acids
40. Qualitative and Quantitative Determination of Sialic Acids
41. Determination of O-Acetyl Groups by the Hestrin Methods
42. Determination of Hexosamines and Hexosaminitols Using the Amino Acid Analyzer
43. Immunofluorescent Identification of Complex Carbohydrates
Section IV. Selected Methods Found in Other Collections
Text
Glossary
Author Index
Subject Index
Product details
- No. of pages: 316
- Language: English
- Copyright: © Academic Press 1976
- Published: January 28, 1976
- Imprint: Academic Press
- eBook ISBN: 9780323156387
About the Editor
Roy Whistler
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