General methods, glycosaminoglycans, and glycoproteins

Methods in Carbohydrate Chemistry, Volume VII: General Methods, Glycosaminoglycans, and Glycoproteins is a compilation of useful methods from the entire field of carbohydrate chemistry.

This volume puts emphasis on methods and procedures used with glycoproteins and glycosaminoglycans. The book contains chapters devoted to the presentation of methods and procedures used with mono- and disaccharides, glycosides and nucleosides, and amino sugars. The methods for the isolation, purification, and analysis of glycoproteins and glycosaminoglycans are elaborated in detail as well.

This volume is intended for the use of chemists, biochemists, and researchers in the field of carbohydrate chemistry.

Contributors to Volume VII

Preface

Outline of Volumes I-VI

Errata and Additions—Volumes I-VI

Section I. Mono- and Disaccharides

Preparation of Mono- and Disaccharides

1. D-Allose

Glycosides and Nucleosides

2. Methyl α- and β-D-Glucoseptanosides

3. Methyl α-Maltoside

4. Preparation of 2′-Deoxyribonucleosides via Nucleoside Deoxyribosyl Transferase

Amino Sugars

5. 2-Acetamido-2-Deoxy-D-Allose and 2-Acetamido-2-Deoxy-D-altrose

6. 2-Acetamido-2-Deoxy-D-Aulose and 2-Acetamido-2-Deoxy-D-Idose

7. 2-Acetamido-2-Deoxy-D-Galactose and 2-Acetamido-2-Deoxy-D-Talose

Thio Sugars

8. Thio Sugars from Oxidatively Coupled Xanthates

9. Thio Sugars from Cyclic Thionocarbonates

Oxidation Products

10. 3-Deoxy-D-manno-Octulosonic Acid

Deoxyhalo Sugars

11. Direct Replacement of Primary Hydroxyl Groups by Halogen

Esters

12. 1-O-Acetyl-2,3,5-Tri-O-Benzoyl-D-Arabino-Furanose and its Use in Glycosylation by Fusion

Ethers and Anhydrosugars

13. Triphenylmethylation with Tritylpyridinium Flouroborate

14. 2,5-Anhydro-D-Mannitol

Analysis

15. Radiochromatographic Analysis of Reducing Carbohydrates by Radiochromatography

16. Determination of Mono- and Disaccharides in Plant Materials

17. Determination of Anomeric Configuration by nmr

Section II. Oligo- and Polysaccharides

Isolation and Purification of Glycosaminoglycans

18. Heparitin (Heparan) Sulfate Isolation from Beef Lung

Physical Methods of Analysis

19. Analysis and Characterization of Glycosaminoglycans by Nuclear Magnetic Resonance Spectroscopy

20. Microanalysis and Characterization of Glycosaminoglycans from Human Tissue via Zone Electrophoresis

21. Determination of the Molecular Weight of Connective Tissue Glycosaminoglycans (Acid Mucopolysaccharides) by Gel Electrophoresis

22. Circular Dichroism and Optical Rotatory Dispersion in Polysaccharide Structural Analysis: Intrinsic and Extrinsic Cotton Effects

Chemical Methods of Analysis

23. Nitrous Acid Depolymerization of Glycosaminoglycans

24. Specific Degradation of Polysaccharides Containing Uronic Acid Residues

25. Stoichiometric Reduction of Uronic Acid Carboxyl Groups in Polysaccharides

26. Distribution of D-Galactopyranosyl Units in Galactomannans

27. Acyl Localization via Methyl Replacement

Section III. Glycoproteins

Isolation and Purification

28. Isolation of Fetuin

29. Ribonuclease B

30. Ovalbumin of Chicken Egg White

31. Stem Bromelain

Analysis of Glycoproteins

32. Isolation of Glycopeptides from Glycoproteins by Proteolytic Digestion

33. Isolation of Glycopeptides from Cell Membrane Glycoproteins

34. Acid-Catalyzed Hydrolysis and Methanolysis of Glycoproteins

35. Determination of O-Glycosidic Linkages to L-Serine and L-Threonine Residues of Glycoproteins

36. Determination of the 5-O-β-D-Galactopyranosylhydroxy-L-Lysine Linkage in Glycoproteins

37. Determination of the 4-N-2-Acetamido-2-Deoxy-β-D-Glucopyranosyl-L-Asparagine Linkage in Glycoproteins

38. Enzymic Methods for the Structure Analysis of the Carbohydrate Chain of Glycoproteins

39. Removal of Sialic Acids from Glycoproteins by Chemical Methods and Determination of Sialic Acids

40. Qualitative and Quantitative Determination of Sialic Acids

41. Determination of O-Acetyl Groups by the Hestrin Methods

42. Determination of Hexosamines and Hexosaminitols Using the Amino Acid Analyzer

43. Immunofluorescent Identification of Complex Carbohydrates

Section IV. Selected Methods Found in Other Collections

Text

Glossary

Author Index

Subject Index