General methods, glycosaminoglycans, and glycoproteins

General methods, glycosaminoglycans, and glycoproteins

1st Edition - January 28, 1976

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  • Editor: Roy Whistler
  • eBook ISBN: 9780323156387

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Methods in Carbohydrate Chemistry, Volume VII: General Methods, Glycosaminoglycans, and Glycoproteins is a compilation of useful methods from the entire field of carbohydrate chemistry. This volume puts emphasis on methods and procedures used with glycoproteins and glycosaminoglycans. The book contains chapters devoted to the presentation of methods and procedures used with mono- and disaccharides, glycosides and nucleosides, and amino sugars. The methods for the isolation, purification, and analysis of glycoproteins and glycosaminoglycans are elaborated in detail as well. This volume is intended for the use of chemists, biochemists, and researchers in the field of carbohydrate chemistry.

Table of Contents

  • Contributors to Volume VII


    Outline of Volumes I-VI

    Errata and Additions—Volumes I-VI

    Section I. Mono- and Disaccharides

    Preparation of Mono- and Disaccharides

    1. D-Allose

    Glycosides and Nucleosides

    2. Methyl α- and β-D-Glucoseptanosides

    3. Methyl α-Maltoside

    4. Preparation of 2′-Deoxyribonucleosides via Nucleoside Deoxyribosyl Transferase

    Amino Sugars

    5. 2-Acetamido-2-Deoxy-D-Allose and 2-Acetamido-2-Deoxy-D-altrose

    6. 2-Acetamido-2-Deoxy-D-Aulose and 2-Acetamido-2-Deoxy-D-Idose

    7. 2-Acetamido-2-Deoxy-D-Galactose and 2-Acetamido-2-Deoxy-D-Talose

    Thio Sugars

    8. Thio Sugars from Oxidatively Coupled Xanthates

    9. Thio Sugars from Cyclic Thionocarbonates

    Oxidation Products

    10. 3-Deoxy-D-manno-Octulosonic Acid

    Deoxyhalo Sugars

    11. Direct Replacement of Primary Hydroxyl Groups by Halogen


    12. 1-O-Acetyl-2,3,5-Tri-O-Benzoyl-D-Arabino-Furanose and its Use in Glycosylation by Fusion

    Ethers and Anhydrosugars

    13. Triphenylmethylation with Tritylpyridinium Flouroborate

    14. 2,5-Anhydro-D-Mannitol


    15. Radiochromatographic Analysis of Reducing Carbohydrates by Radiochromatography

    16. Determination of Mono- and Disaccharides in Plant Materials

    17. Determination of Anomeric Configuration by nmr

    Section II. Oligo- and Polysaccharides

    Isolation and Purification of Glycosaminoglycans

    18. Heparitin (Heparan) Sulfate Isolation from Beef Lung

    Physical Methods of Analysis

    19. Analysis and Characterization of Glycosaminoglycans by Nuclear Magnetic Resonance Spectroscopy

    20. Microanalysis and Characterization of Glycosaminoglycans from Human Tissue via Zone Electrophoresis

    21. Determination of the Molecular Weight of Connective Tissue Glycosaminoglycans (Acid Mucopolysaccharides) by Gel Electrophoresis

    22. Circular Dichroism and Optical Rotatory Dispersion in Polysaccharide Structural Analysis: Intrinsic and Extrinsic Cotton Effects

    Chemical Methods of Analysis

    23. Nitrous Acid Depolymerization of Glycosaminoglycans

    24. Specific Degradation of Polysaccharides Containing Uronic Acid Residues

    25. Stoichiometric Reduction of Uronic Acid Carboxyl Groups in Polysaccharides

    26. Distribution of D-Galactopyranosyl Units in Galactomannans

    27. Acyl Localization via Methyl Replacement

    Section III. Glycoproteins

    Isolation and Purification

    28. Isolation of Fetuin

    29. Ribonuclease B

    30. Ovalbumin of Chicken Egg White

    31. Stem Bromelain

    Analysis of Glycoproteins

    32. Isolation of Glycopeptides from Glycoproteins by Proteolytic Digestion

    33. Isolation of Glycopeptides from Cell Membrane Glycoproteins

    34. Acid-Catalyzed Hydrolysis and Methanolysis of Glycoproteins

    35. Determination of O-Glycosidic Linkages to L-Serine and L-Threonine Residues of Glycoproteins

    36. Determination of the 5-O-β-D-Galactopyranosylhydroxy-L-Lysine Linkage in Glycoproteins

    37. Determination of the 4-N-2-Acetamido-2-Deoxy-β-D-Glucopyranosyl-L-Asparagine Linkage in Glycoproteins

    38. Enzymic Methods for the Structure Analysis of the Carbohydrate Chain of Glycoproteins

    39. Removal of Sialic Acids from Glycoproteins by Chemical Methods and Determination of Sialic Acids

    40. Qualitative and Quantitative Determination of Sialic Acids

    41. Determination of O-Acetyl Groups by the Hestrin Methods

    42. Determination of Hexosamines and Hexosaminitols Using the Amino Acid Analyzer

    43. Immunofluorescent Identification of Complex Carbohydrates

    Section IV. Selected Methods Found in Other Collections



    Author Index

    Subject Index

Product details

  • No. of pages: 316
  • Language: English
  • Copyright: © Academic Press 1976
  • Published: January 28, 1976
  • Imprint: Academic Press
  • eBook ISBN: 9780323156387

About the Editor

Roy Whistler

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