General Cytochemical Methods

General Cytochemical Methods

1st Edition - January 1, 1961

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  • Editor: J.F. Danielli
  • eBook ISBN: 9780323156554

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General Cytochemical Methods, Volume II focuses on methods and techniques employed in the studies of the biochemistry of cells. Composed of eight chapters, the book looks at immersion refractometry of living cells by phase contrast and interference microscopy. Areas considered include interpretation of refractive index measurements as indicator of hydration; immersion refractometry with phase-contrast microscopy; and practical aspects of checking phase-change with interference microscopes. The text continues with the discussions on the Cartesian diver balance method. Particularly noted are the standards, principle, applications, and precision of the method, which has been proven effective in microgasometric measurements. The book also focuses on quantitative determination through a special ""ampulla-diver"" of the cholinesterase activity in cells. Given attention are the materials, methods, and results of experiments. The text also looks at periodate oxidation techniques; acylation and diazonium coupling in protein cytochemistry with special reference to the benzoylation-tetrazonium method; and the use of dinitrobenzene as a cytochemical reagent. The book is a great find for readers interested in studying the biochemistry of cells.

Table of Contents

  • Preface

    List of Contributors

    The Immersion Refractometry of Living Cells by Phase Contrast and Interference Microscopy

    I. Introduction and Theory of the Method

    II. The Interpretation of Refractive Index Measurements as an Indication of Hydration

    III. Suitable Mounting Media for the Immersion Refractometry of Living Cells

    IV. Immersion Refractometry with Phase Contrast Microscopy

    V. Immersion Refractometry by Interference Microscopy

    VI. Appendix: Some Practical Aspects of Measuring Phase-change with Interference Microscopes


    The Cartesian Diver Balance

    I. Principle of Method

    II. Making the Diver Balance

    III. Selection of Diver for Use

    IV. Making the Diver Balance Float

    V. The Standards

    VI. The Actual Weighing

    VII. Sensitivity and Precision of Method

    VIII. What is Measured by the RW

    IX. Applications of Method

    X. Conclusions


    The Cartesian Diver

    I. Introduction

    II. Standard Diver (μΙ-Diver)

    III. Theory of the Diver

    IV. Measurement of V and Ρ

    V. Applications of the Standard Diver


    Quantitative Determination by a Special "Ampulla-Diver" of Cholinesterase Activity in Individual Cells, with Notes on Other Uses of the Method

    I. Introduction

    II. Material and Methods

    III. Results

    IV. Comments

    V. Summary

    VI. Notes


    Localization of Deoxyribonuclease Activity by the Substrate Film Method

    I. Introduction

    II. Theory of the Method

    III. Instrumentation

    IV. Procedure

    V. Critique of the Method

    VI. Results

    VII. Appendix: Materials and Solutions


    Periodate Oxidation Techniques

    I. Introduction

    II. Theory of Method

    III. Procedures of Origin

    IV. Critique

    V. Accessory Observations

    VI. Appendix: Further Discussion of Procedures


    Acylation and Diazonium Coupling in Protein Cytochemistry with Special Reference to the Benzoylation-Tetrazonium Method

    I. Introduction

    II. Theory of the Methods

    III. Instrumentation and Problems of Measurement

    IV. Procedures, and the Effects of Variables Therein

    V. Critique of the Method

    VI. Assessment of Results to Date

    VII. Appendices


    1-Fluoro-2:4-Dinitrobenzene as a Cytochemical Reagent

    I. Introduction

    II. Theory of Method

    III. Histological Procedure

    IV. Analytical Investigation of the Reactive Components in Nuclei

    V. Critique of Method

    VI. Results to Date


    Author Index

    Subject Index

Product details

  • No. of pages: 310
  • Language: English
  • Copyright: © Academic Press 1961
  • Published: January 1, 1961
  • Imprint: Academic Press
  • eBook ISBN: 9780323156554

About the Editor

J.F. Danielli

Affiliations and Expertise

Zoology Department King’s College London, England

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