General Cytochemical Methods, Volume II focuses on methods and techniques employed in the studies of the biochemistry of cells. Composed of eight chapters, the book looks at immersion refractometry of living cells by phase contrast and interference microscopy. Areas considered include interpretation of refractive index measurements as indicator of hydration; immersion refractometry with phase-contrast microscopy; and practical aspects of checking phase-change with interference microscopes. The text continues with the discussions on the Cartesian diver balance method. Particularly noted are the standards, principle, applications, and precision of the method, which has been proven effective in microgasometric measurements. The book also focuses on quantitative determination through a special ""ampulla-diver"" of the cholinesterase activity in cells. Given attention are the materials, methods, and results of experiments. The text also looks at periodate oxidation techniques; acylation and diazonium coupling in protein cytochemistry with special reference to the benzoylation-tetrazonium method; and the use of dinitrobenzene as a cytochemical reagent. The book is a great find for readers interested in studying the biochemistry of cells.
Preface List of Contributors The Immersion Refractometry of Living Cells by Phase Contrast and Interference Microscopy I. Introduction and Theory of the Method II. The Interpretation of Refractive Index Measurements as an Indication of Hydration III. Suitable Mounting Media for the Immersion Refractometry of Living Cells IV. Immersion Refractometry with Phase Contrast Microscopy V. Immersion Refractometry by Interference Microscopy VI. Appendix: Some Practical Aspects of Measuring Phase-change with Interference Microscopes References The Cartesian Diver Balance I. Principle of Method II. Making the Diver Balance III. Selection of Diver for Use IV. Making the Diver Balance Float V. The Standards VI. The Actual Weighing VII. Sensitivity and Precision of Method VIII. What is Measured by the RW IX. Applications of Method X. Conclusions References The Cartesian Diver I. Introduction II. Standard Diver (μΙ-Diver) III. Theory of the Diver IV. Measurement of V and Ρ V. Applications of the Standard Diver References Quantitative Determination by a Special "Ampulla-Diver" of Cholinesterase Activity in Individual Cells, with Notes on Other Uses of the Method I. Introduction II. Material and Methods III. Results IV. Comments V. Summary VI. Notes References Localization of Deoxyribonuclease Activity by the Substrate Film Method I. Introduction II. Theory of the Method III. Instrumentation IV. Procedure V. Critique of the Method VI. Results VII. Appendix: Materials and Solutions References Periodate Oxidation Techniques I. Introduction II. Theory of Method III. Procedures of Origin IV. Critique V. Accessory Observations VI. Appendix: Further Discussion of Procedures References Acylation and Diazonium C
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- © Academic Press 1961
- 1st January 1961
- Academic Press
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Zoology Department King’s College London, England