Gene Probes for Bacteria

1st Edition - January 1, 1990
Editor: Alberto Macario
Language: English
eBook ISBN:
9 7 8 - 0 - 3 2 3 - 1 4 6 6 9 - 2

Gene Probes for Bacteria focuses on the preparation and use of nucleic acid probes for identifying bacteria in clinical specimens and in other samples of practical or scientific… Read more

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Gene Probes for Bacteria focuses on the preparation and use of nucleic acid probes for identifying bacteria in clinical specimens and in other samples of practical or scientific interest. Organized into 17 chapters, this book addresses which nucleic acid probes are available; how and when to utilize them; what to expect in terms of results obtained with their use; and how to prepare probes. Each chapter is composed of comprehensive manuscript covering different microorganisms. A few bacterial species are treated in more than one chapter to provide a multidimensional picture of important microbes, and to compile the knowledge gained from different laboratories using the same or different probes or methods. This reference material will be of great use to professionals, technicians, and R&D directors in universities, federal and state-dependent service and research institutes, and private and industrial laboratories.

Contributors

Preface

Introduction

1 The Use of Nonradioactive DNA Probes for Rapid Diagnosis of Sexually Transmitted Bacterial Infections

I. Introduction

II. Background

III. Results and Discussion

IV. Conclusions

V. Gene Probes versus Antisera and Monoclonal Antibodies

VI. Prospects for the Future

VII. Summary

VIII. Materials and Methods

References

2 Detection of Chlamydia trachomatis with DNA Probes

I. Introduction

II. Background

III. Results and Discussion

IV. Conclusions

V. Gene Probes versus Monoclonal Antibodies

VI. Prospects for the Future

VII. Summary

VIII. Materials and Methods

References

3 Construction of DNA Probes for the Identification of Haemophilus ducreyi

I. Introduction

II. Background

III. Results and Discussion

IV. Conclusions

V. Gene Probes versus Antisera and Monoclonal Antibodies

VI. Prospects for the Future

VII. Summary

VIII. Materials and Methods

References

4 Detection of Diarrheogenic Escherichia coli Using Nucleotide Probes

I. Introduction

II. Background

III. Results and Discussion

IV. Conclusions

V. Gene Probes versus Immunological Assays

VI. Prospects for the Future

VII. Summary

VIII. Materials and Methods

References

5 DNA Probes for Escherichia coli Isolates from Human Extraintestinal Infections

I. Introduction

II. Background

III. Results and Discussion

IV. Conclusions

V. Gene Probes Compared with Other Methods of Detection

VI. Prospects for the Future

VII. Summary

VIII. Materials and Methods

References

6 Identification of Enterotoxigenic Escherichia coli by Colony Hybridization Using Biotinylated LTIh, STIa, and STIb Enterotoxin Probes

I. Introduction

II. Background

III. Results and Discussion

IV. Conclusions

V. Gene Probes versus Antisera and Monoclonal Antibodies

VI. Prospects for the Future

VII. Summary

References

7 Early days in the Use of DNA Probes for Mycobacterium tuberculosis and Mycobacterium avium Complexes

I. Introduction

II. Background

III. Results and Discussion

IV. Conclusions

V. Gene Probes versus Antisera and Monoclonal Antibodies

VI. Prospects for the Future

VII. Summary

VIII. Materials and Methods

References

8 Att Sites, Tox Gene, and Insertion Elements as Tools for the Diagnosis and Molecular Epidemiology of Corynebacterium diphtheriae

I. Introduction

II. Background

III. Results and Discussion

IV. Conclusions and Prospects for the Future

V. Summary

VI. Materials and Methods

References

9 Nucleic Acid Probes for Bacteroides Species

I. Introduction

II. Background

III. Results and Discussion

IV. Conclusions

V. Gene Probes versus Monoclonal Antibodies

VI. Prospects for the Future

VII. Summary

VIII. Materials and Methods

References

10 Nucleic Acid Probes for Campylobacter Species

I. Introduction

II. Background

III. Results and Discussion

IV. Conclusions

V. Gene Probes versus Antisera and Monoclonal Antibodies

VI. Prospects for the Future

VII. Summary

VIII. Materials and Methods

References

11 Detection of Leptospira, Haemophilus, and Campylobacter Using DNA Probes

I. Introduction

II. Leptospirosis

III. Haemophilus

IV. Campylobacter

V. Conclusions

VI. Prospects for the Future

VII. Summary

VIII. Materials and Methods

References

12 Nucleic Acid Probes for the Identification of Salmonella

I. Introduction

II. Background

III. Results and Discussion

IV. Conclusions

V. Gene Probes versus Antisera and Monoclonal Antibodies

VI. Prospects for the Future

VII. Summary

References

13 Gene Probes for Detection of Food-Borne Pathogens

I. Introduction

II. Background

III. Results and Discussion

IV. Conclusions

V. Hybridization Assay versus Conventional Assays

VI. Prospects for the Future

VII. Summary

VIII. Materials and Methods

References

14 The Use of Gene and Antibody Probes in Identification and Enumeration of Rumen Bacterial Species

I. Introduction

II. Background

III. Results and Discussion

IV. Conclusions

V. Gene Probes versus Antisera and Monoclonal Antibodies: Prospects for the Future

VI. Summary

VII. Materials and Methods

References

15 Gene Probe Detection of Human and Cell Culture Mycoplasmas

I. Introduction

II. Background

III. Results and Discussion

IV. Conclusions

V. Gene Probes versus Antisera and Monoclonal Antibodies

VI. Prospects for the Future

VII. Summary

VIII. Materials and Methods

References

16 Detection of TEM β-Lactamase Genes Using DNA Probes

I. Introduction

II. Background

III. Results and Discussion

IV. Conclusions

V. Gene Probes versus Antisera and Monoclonal Antibodies

VI. Prospects for the Future

VII. Summary

VIII. Materials and Methods

References

17 SIA Technology for Probing Microbial Genes

I. Introduction

II. Background

III. Results and Discussion

IV. Conclusions

V. Prospects for the Future

VI. Summary

VII. Materials and Methods

References

Index