Gene Manipulations in Fungi

Gene Manipulations in Fungi

1st Edition - December 10, 1985

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  • Editor: J.W. Bennett
  • eBook ISBN: 9780323145411

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Gene Manipulations in Fungi combines a review of classical fungal genetics, contemporary research, and responsible speculation about the future. This book focuses on yeasts and molds; because yeast is the primary model system for eukaryotes and that there is an elegant research on molds. The applications of fungi, including their economic importance, are addressed. The book emphasizes the need for improved transformation systems, appropriate vectors, and broadly applicable selectable markers in this field of interest. This book will help stimulate the development of innovative approaches in this subject matter.

Table of Contents

  • Contributors



    I Historical Perspective: Mutants to Models

    1 From Auxotropic Mutants to DNA Sequences

    I. The Establishment of the Paradigm: One Gene-One Polypeptide

    II. Cluster Genes and Gene Clusters

    III. Systems of Regulation of Gene Activity

    IV. Cloning and Sequencing


    2 Molecular Taxonomy of the Fungi

    I. Introduction

    II. Nucleic Acid Isolation and Purification

    III. DNA Base Composition

    IV. DNA Relatedness

    V. Mitochondrial DNA Relatedness

    VI. Ribosomal RNA Relatedness

    VII. Comparison of Relatedness from Nucleic Acid Studies with That Determined by Other Methodologies


    3 Fungal Mitochondrial Genomes

    I. Introduction

    II. Physical Organization

    III. Genes and the Genetic Code

    IV. Gene Order

    V. Transcription

    VI. Pseudogenes

    VII. Mitochondrial Plasmids

    VIII. Effects of Alteration of mtDNA

    IX. Nuclear-Cytoplasmic Interactions


    4 Modeling the Environment for Gene Expression

    I. Introduction

    II. Modeling Metabolism under Steady-State Conditions

    III. Modeling Metabolism during Product Accumulation


    II Yeasts

    5 Saccharomyces cerevisiae as a Paradigm for Modern Molecular Genetics of Fungi

    I. Introduction

    II. Neoclassical Genetics

    III. Transformation

    IV. Cloning a Gene

    V. Manipulation of a Cloned Gene

    VI. Other Uses of Cloned Genes

    VII. An Agenda for Progress


    6 Yeast Transformation

    I. Introduction

    II. Specific Transformation Systems in Yeast

    III. Applications

    IV. Conclusions


    7 Use of the LYS2 Gene for Gene Disruption, Gene Replacement, and Promoter Analysis in

    Saccharomyces cerevisiae

    I. Introduction

    II. Characterization of the LYS2 Gene and Its Product

    III. Genetic Manipulations Utilizing the LYS2 Gene

    IV. Conclusions and Prospectus


    III Molds

    8 Molecular Biology of the qa Gene Cluster of Neurospora

    I. Introduction

    II. Molecular Analysis of the qa Cluster

    III. Transformation of the qa Gene Cluster in Neurospora

    IV. Summary


    9 Neurospora Plasmids

    I. Introduction

    II. Naturally Occurring Plasmids

    III. Replicating Plasmids Constructed in the Laboratory

    IV. Overview and Prospects for the Future


    10 Cloning and Transformation in Aspergillus

    I. Introduction

    II. Transformation Methodology

    III. Fate of Transforming DNA after Entry

    IV. The Quest for Replicating Vectors

    V. Development of Vectors

    VI. Future Prospects


    11 Expression of Aspergillus Genes in Neurospora

    I. Transformation of Neurospora crassa

    II. Isolation of the Aspergillus nidulans Gene for Ornithine Carbamoyltransferase

    III. Transformation of the Neurospora crassa arg-12 Mutant . .

    IV. Control of Gene Expression

    V. Subcellular Localization of the Active Enzyme

    VI. Conclusions


    12 Gene Dosage Effects and Antibiotic Synthesis in Fungi

    I. Introduction

    II. Methods for the Amplification of Genetic Material in Microorganisms

    III. Studies with Disomic Strains of Aspergillus nidulans


    13 Formal Genetics and Molecular Biology of the Control of Gene Expression in Aspergillus nidulans

    I. Formal Genetic Methodology of Aspergillus nidulans as Applied to the Study of Control Systems

    II. The Metabolic Versatility of A. nidulans and Its Exploitation

    III. Regulatory Genes

    IV. Putative Receptor Sites

    V. The Spatial Organization of Functionally Related Genes

    VI. At What Level Does Regulation of Gene Expression Occur?


    14 A Cloning Strategy in Filamentous Fungi



    IV Applications

    15 Primary Metabolism and Industrial Fermentations

    I. Primary Metabolites

    II. Genetic Approaches to the Production of Primary Metabolites

    III. Organic Acids

    IV. Amino Acids

    V. Polysaccharides

    VI. Lipids

    VII. Nucleotides and Nucleic Acid-Related Compounds

    VIII. Vitamins

    IX. Polyols

    X. Ethanol

    XI. The Promise of Biotechnology


    16 Mitochondrial DNA for Gene Cloning in Eukaryotes

    I. Introduction

    II. Fungal Plasmids

    III. Cloning Vectors of Mitochondrial Origin

    IV. Biotechnological Implications


    17 Molecular Bases of Fungal Pathogenicity to Plants

    I. Introduction

    II. Infection Structures

    III. Cutinase

    IV. Pisatin Demethylase

    V. Toxins

    VI. Cloning and Analysis of Pathologically Important Genes from Fungi


    18 Morphogenesis and Dimorphism of Mucor

    I. Introduction

    II. Dimorphism of Mucor

    III. Molecular Analysis of Mucor

    IV. Perspectives—Molecular Genetics


    19 Toward Gene Manipulations with Selected Human Fungal Pathogens

    I. Introduction

    II. Cryptococcus neoformans

    III. Histoplasma capsulatum

    IV. Wangiella dermatitidis

    V. Candida albicans

    VI. Concluding Remarks


    20 Fungal Carbohydrases: Amylases and Cellulases

    I. Introduction

    II. Fungal Amylases

    III. Fungal Cellulases

    IV. Summary and Outlook


    V Postscript

    21 Prospects for a Molecular Mycology

    I. Introduction

    II. A Primer in Mycology

    III. Molecular Mycology



    I Fungal Taxonomy

    I. Introduction

    II. Outline of Fungal Taxonomy


    II Conventions for Gene Symbols

    I. Aspergillus nidulans

    II. Neurospora crassa

    III. Saccharomyces cerevisiae

    IV. Other Fungi



Product details

  • No. of pages: 578
  • Language: English
  • Copyright: © Academic Press 1985
  • Published: December 10, 1985
  • Imprint: Academic Press
  • eBook ISBN: 9780323145411

About the Editor

J.W. Bennett

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