Description

Gene Expression Systems: Using Nature for the Art of Expression offers detailed information on a wide variety of gene expression systems from an array of organisms. It describes several different types of expression systems including transient, stable, viral, and transgenic systems. Each chapter is written by a leader in the field. The book includes timelines and examples for each expression system, and provides an overview of the future of recombinant protein expression.

Key Features

@introbul:Key Features @bul:* Provides detailed information on expression systems * Covers a variety of promoters and host organisms enabling researchers to tailor protocols to their specific needs * Includes timelines and examples * Compares pros and cons of each method

Readership

Molecular Biologists, Biochemists, Protein Chemists, and Biophysicists.

Table of Contents

Contributors. Introduction: The Art of Expression. Purpose of This Book. Selecting a Suitable Expression System: Considerations. Genomics and the Future of Protein Expression Systems, J.M. Fernandez and J.P. Hoeffler. Prokaryotic Expression Systems: Gene Expression Systems Based on Bacteriophage T7 RNA Polymearse, R. Durbin. Expression Vectors Employing the trc Promoter, J. Brosius. Bacillus Expression: A Gram Positive Model, E. Ferrari and B. Miller. araB Expression System in Escherichia coli, M. Better. Eukaryotic Expression Systems: Adenoviral Vectors for Protein Expression, D.J. von Seggern and G.R. Nemerow. Expression in the Methalotrophic Yeast Pichia pastoris, J.M. Cregg. Recombinant Protein Expression in Pichia methanolica, C.K. Raymond. Cytomegalovirus Promoter for Expression in Mammalian Cells, M.F. Stinski. Inducible Mammalian Expression Systems, M.Russell. Protein Expression in Mammalian Cells Using Sindbis Virus, R.P. Bennett. Expression in Insect Systems: Drosophila S2 System for Heterologous Gene Expression, R.B. Kirkpatrick and A. Shatzman. Baculovirus Expression Vector System, M. Galleno and A.J. Sick. Transgenic Expression: Recombinant Protein Expression in Transgenic Mice, R. Abbud and J.H. Nilson. Expression of Recombinant Proteins in the Milk of Transgenic Animals, H.M. Meade, Y. Echelard, C.A. Ziomek, M.W. Young, M. Harvey, E.S. Cole, S. Groet, T.E. Smith, and J.M. Curling. Recombinant Protein Expression

Details

No. of pages:
480
Language:
English
Copyright:
© 1999
Published:
Imprint:
Academic Press
eBook ISBN:
9780080532356
Print ISBN:
9780122538407
Print ISBN:
9780123992161

About the editors

Joseph Fernandez

Joseph Fernandez is the Vice President of Business Development at Invitrogen Corporation in Carlsbad, California, where he identifies and licenses new technologies including gene expression systems.

Affiliations and Expertise

Invitrogen Corporation, Carlsbad, California, U.S.A.

James Hoeffler

Jim Hoeffler is the Director of Research and Development at Invitrogen Corporation, concentrating on the development of new products, with a focus on gene expression systems.

Affiliations and Expertise

Invitrogen Corporation, Carlsbad, California, U.S.A.