This third volume in the trio covering G proteins, features integrated approaches to studying G proteins. Methods pertaining to signaling mechanisms are presented, including theoretical and modeling approaches, biochemistry and molecular biology, and cell biology and physiology. The techniques for studying the structure and function of G proteins are important not only to those with specific research interests in them, but also endocrinologists and pharmacologists conducting research on signaling mechanisms that are increasingly understood to interact with G proteins.


Biochemists, molecular biologists, cell biologists, pharmacologists, neurophysiologists, neurochemists, neuroendocrinologists, and biomedical researchers.

Table of Contents

Contributors to Volume 345. Preface. Volume in Series. Section I: Modeling Intracellular Signaling Pathways: Use of Kinetikit and GENESIS for Modeling Signaling Pathways. Section II: Phosphodiesterases: Assays of G Protein/cGMP-Phosphodiesterase Interactions. Assaying G Protein-Phosphodiesterase Interactions in Sensory Systems. Section III: Calcium and Potassium Channels: Studies of Endogenous G-Protein-Mediated Pathways in Neurons by Whole-Cell Electrophysiology. Biochemical Approaches to Study Interaction of Calcium Channels with RGS12 in Primary Neuronal Cultures. Assaying Phosphatidylinositol Bisphosphate Regulation of Potassium Channels. Section IV: Adenylyl Cyclases: Purification of Soluble Adenylyl Cyclase. Calcium-Sensitive Adenylyl Cyclase/Aequorin Chimeras as Sensitive Probes for Discrete Modes of Elevation of Cytosolic Calcium. Kinetic Analysis of the Action of P-Site Analogs. Expression, Purification, and Assay of Cytosolic (Catalytic) Domains of Membrane-Bound Mammalian Adenylyl Cyclases. Identification of Putative Direct Effectors for Ga0 Using Yeast Two-Hybrid Method. Identification of Transmembrane Adenylyl Cyclase Isoforms. Functional Analyses of Type V Adenylyl Cyclase. Photoaffinity Labeling of Adenylyl Cyclase. Crystallization of Complex between Soluble Domains of Adenylyl Cyclase and Activated Gsa. Generation of Adenylyl Cyclase Knockout Mice. Construction of Soluble Adenylyl Cyclase from Human Membrane-Bound Type 7 Adenylyl Cyclase. Section V: Phospholipases and Lipid-Derived Products:<


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© 2002
Academic Press
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About the serial-volume-editors

Ravi Iyengar

Affiliations and Expertise

Mount Sinai School of Medicine, New York, U.S.A.

John Hildebrandt

Affiliations and Expertise

Medical University of South Carolina, Charleston, U.S.A.