Description

This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers G protein coupled receptors and includes chapters on such topics as post-translation modification of GPCR in relationship to biased agonism, structure-based virtual screening, and GPCR oligomerization in the brain.

Key Features

  • Continues the legacy of this premier serial with quality chapters authored by leaders in the field
  • Covers G protein coupled receptors
  • Contains chapters on such topics as post-translation modification of GPCR in relationship to biased agonism, structure-based virtual screening, and GPCR oligomerization in the brain

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of Contents

Series Page

Contributors

Preface

Methods in Enzymology

Chapter One. Expression of GPCRs in Pichia pastoris for Structural Studies

1 Introduction

2 Generation of Expression Clones

3 Screening Transformants

4 Characterization and Optimization of Initial Target Receptor Production

5 Large-Scale Production

6 Prospects for Large-Scale GPCR Production in P. pastoris

References

Chapter Two. Conformational Ensemble View of G Protein-Coupled Receptors and the Effect of Mutations and Ligand Binding

1 Introduction

2 Overview of the Conformational Ensemble Prediction

3 Generating Starting Structures Based on Templates

4 BiHelix: TM Bundle Conformational Sampling of Helix Rotation Angles

5 SuperBiHelix TM Bundle Sampling of All Helix Orientation Angles

6 Effect of Mutations on the WT Conformational Ensemble

7 Ensemble Docking of Ligands to WT or Mutant Receptors

8 Summary

References

Chapter Three. Structural Evolution of G-Protein-Coupled Receptors

Abbreviations

1 Introduction

2 The Puzzle of GPCR Evolution

3 The Evolution of Class A GPCRs Viewed by MDS

4 Evolutionary Trends

5 Structural Evolution of GPCRs

6 Summary

References

Chapter Four. Directed Evolution of G-Protein-Coupled Receptors for High Functional Expression and Detergent Stability

1 Introduction

2 Methods

References

Chapter Five. The Role of Hydrophobic Amino Acids in the Structure and Function of the Rhodopsin Family of G Protein-Coupled Receptors

1 Introduction

2 The Structure of GPCRs

3 Sequence Analyses of the 7TM Segments of the Rhodopsin Family of GPCRs

4 Importance of the Highly Conserved Hydrophobic Amino Acid at Position 3.40 in the Process of Agonist-Induced Receptor Activation

5 The Hydro

Details

No. of pages:
440
Language:
English
Copyright:
© 2013
Published:
Imprint:
Academic Press
Print ISBN:
9780123918611
Electronic ISBN:
9780123918727