G Protein-Coupled Receptors, Part B

G Protein-Coupled Receptors, Part B

2nd Edition - January 5, 2019

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  • Editor: Arun Shukla
  • eBook ISBN: 9780128151082
  • Hardcover ISBN: 9780128151075

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G-Protein-Coupled Receptors, Part B, 2nd Edition, Volume 149, the latest release in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers Optical Approaches for Visualization of Arrestin Binding to Muscarinic Receptors, Luciferase Reporter Assay for Unlocking Ligand-mediated Signaling of GPCRs, Assays to Measure GPCR Dependent Cellular Migration, Characterization of the Frizzled GPCRs, Binding Assays for Bradykinin and Angiotensin Receptors, Detection of Misfolded Rhodopsin Aggregates in Cells, Measuring GPCR Ubiquitination and Trafficking, Culture of Primary Neurons and its Use in Studying GPCR Trafficking, and much more.

Key Features

  • Covers the increasingly appreciated cell biology field of G-protein-coupled receptors
  • Includes both established and new technologies
  • Contributed by experts in the field


Researchers and students in cell, molecular and developmental biology

Table of Contents

  • 1. Optical approaches for visualization of arrestin binding to muscarinic receptor
    Seung Ryoung Jung
    2. Luciferase Reporter Assay for Unlocking Ligand-mediated Signaling of GPCRs
    Hamiyet Unal
    3. Assays to measure GPCR dependent cellular migration
    Carole A. Parent
    4. Characterization of the Frizzled GPCRs
    Vladimir L. Katanaev
    5. Binding assays for bradykinin and angiotensin receptors
    Renan Martin
    6. Detection of misfolded rhodopsin aggregates in cells
    Paul Park
    7. Measuring GPCR ubiquitination and trafficking
    Samarjit Bhattacharyya
    8. Culture of primary neurons and it use in studying GPCR trafficking
    Prem N. Yadav
    9. ELISA based assay for measuring agonist-induced GPCR endocytosis
    Arun K. Shukla
    10. Western blot assay for measuring agonist-induced ERK1/2 phosphorylation
    Arun K. Shukla
    11. Understanding GPCR dimerization
    Agata Faron-Górecka
    12. Investigating the Frizzled family of receptors
    Vladimir L. Katanaev
    13. Measuring GPCR signaling through xCELLigence system based assay
    Laura Heitman
    14. Novel mechanisms of GPCR signaling and bias
    Gabriel Carmona Rosas
    15. co-IP assays to measure GPCR-arrestin interactions
    Anastasios Lymperopoulos
    16. Investigating arrestin dependent GPCR signaling
    Sadashiva Karnik
    17. Measuring CRH1 receptor signaling
    Susana Silberstein
    18. Site-specific side chain labeling for NMR studies of G protein-coupled receptors
    Brian F. Volkman
    19. Modeling chemokine receptors and their orthosteric site interactions
    Brian F. Volkman
    20. Receptor-channel cross-talk
    Uri Kahanovitch
    21. Wnt receptor signaling
    Nibaldo C. Inestrosa

Product details

  • No. of pages: 332
  • Language: English
  • Copyright: © Academic Press 2019
  • Published: January 5, 2019
  • Imprint: Academic Press
  • eBook ISBN: 9780128151082
  • Hardcover ISBN: 9780128151075

About the Serial Volume Editor

Arun Shukla

Arun Shukla
Dr. Arun K. Shukla obtained his M.Sc. (Master in Science) from the Center for Biotechnology at the Jawaharlal Nehru University in New Delhi, India. Dr. Shukla did his Ph.D. from the Department of Molecular Membrane Biology at the Max Planck Institute of Biophysics in Frankfurt, Germany. His Ph.D. research work was focused on structural studies of G Protein-Coupled Receptors (GPCRs).

Dr. Shukla subsequently carried out his post-doctoral work in the Department of Medicine at the Duke University in North Carolina, USA. During his post-doctoral research work, Dr. Shukla focused on understanding the biophysical and structural basis of ß-arrestin mediated regulation of GPCRs and non-canonical GPCR signaling. Dr. Shukla has served as an Assistant Professor in the Department of Medicine at the Duke University in Durham, North Carolina, USA.

Dr. Shukla is currently an Assistant Professor in Department of Biological Sciences and Bioengineering at the Indian Institute of Technology, Kanpur, India. Dr. Shukla is also an Intermediate Fellow of the Wellcome Trust-DBT India Alliance. The research program in Dr. Shukla’s laboratory is focused on understanding the molecular mechanism of activation, signaling and regulation of G Protein-Coupled Receptors.

Affiliations and Expertise

Assistant Professor, Indian Institute of Technology, Kanpur, India

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