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G Protein-Coupled Receptors, Part B - 2nd Edition - ISBN: 9780128151075, 9780128151082

G Protein-Coupled Receptors, Part B, Volume 149

2nd Edition

Serial Volume Editor: Arun Shukla
eBook ISBN: 9780128151082
Hardcover ISBN: 9780128151075
Imprint: Academic Press
Published Date: 5th January 2019
Page Count: 332
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Table of Contents

1. Optical approaches for visualization of arrestin binding to muscarinic receptor
Seung Ryoung Jung
2. Luciferase Reporter Assay for Unlocking Ligand-mediated Signaling of GPCRs
Hamiyet Unal
3. Assays to measure GPCR dependent cellular migration
Carole A. Parent
4. Characterization of the Frizzled GPCRs
Vladimir L. Katanaev
5. Binding assays for bradykinin and angiotensin receptors
Renan Martin
6. Detection of misfolded rhodopsin aggregates in cells
Paul Park
7. Measuring GPCR ubiquitination and trafficking
Samarjit Bhattacharyya
8. Culture of primary neurons and it use in studying GPCR trafficking
Prem N. Yadav
9. ELISA based assay for measuring agonist-induced GPCR endocytosis
Arun K. Shukla
10. Western blot assay for measuring agonist-induced ERK1/2 phosphorylation
Arun K. Shukla
11. Understanding GPCR dimerization
Agata Faron-Górecka
12. Investigating the Frizzled family of receptors
Vladimir L. Katanaev
13. Measuring GPCR signaling through xCELLigence system based assay
Laura Heitman
14. Novel mechanisms of GPCR signaling and bias
Gabriel Carmona Rosas
15. co-IP assays to measure GPCR-arrestin interactions
Anastasios Lymperopoulos
16. Investigating arrestin dependent GPCR signaling
Sadashiva Karnik
17. Measuring CRH1 receptor signaling
Susana Silberstein
18. Site-specific side chain labeling for NMR studies of G protein-coupled receptors
Brian F. Volkman
19. Modeling chemokine receptors and their orthosteric site interactions
Brian F. Volkman
20. Receptor-channel cross-talk
Uri Kahanovitch
21. Wnt receptor signaling
Nibaldo C. Inestrosa


G-Protein-Coupled Receptors, Part B, 2nd Edition, Volume 149, the latest release in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers Optical Approaches for Visualization of Arrestin Binding to Muscarinic Receptors, Luciferase Reporter Assay for Unlocking Ligand-mediated Signaling of GPCRs, Assays to Measure GPCR Dependent Cellular Migration, Characterization of the Frizzled GPCRs, Binding Assays for Bradykinin and Angiotensin Receptors, Detection of Misfolded Rhodopsin Aggregates in Cells, Measuring GPCR Ubiquitination and Trafficking, Culture of Primary Neurons and its Use in Studying GPCR Trafficking, and much more.

Key Features

  • Covers the increasingly appreciated cell biology field of G-protein-coupled receptors
  • Includes both established and new technologies
  • Contributed by experts in the field


Researchers and students in cell, molecular and developmental biology


No. of pages:
© Academic Press 2019
5th January 2019
Academic Press
eBook ISBN:
Hardcover ISBN:

Ratings and Reviews

About the Serial Volume Editor

Arun Shukla

Arun Shukla

Dr. Arun K. Shukla obtained his M.Sc. (Master in Science) from the Center for Biotechnology at the Jawaharlal Nehru University in New Delhi, India. Dr. Shukla did his Ph.D. from the Department of Molecular Membrane Biology at the Max Planck Institute of Biophysics in Frankfurt, Germany. His Ph.D. research work was focused on structural studies of G Protein-Coupled Receptors (GPCRs).

Dr. Shukla subsequently carried out his post-doctoral work in the Department of Medicine at the Duke University in North Carolina, USA. During his post-doctoral research work, Dr. Shukla focused on understanding the biophysical and structural basis of ß-arrestin mediated regulation of GPCRs and non-canonical GPCR signaling. Dr. Shukla has served as an Assistant Professor in the Department of Medicine at the Duke University in Durham, North Carolina, USA.

Dr. Shukla is currently an Assistant Professor in Department of Biological Sciences and Bioengineering at the Indian Institute of Technology, Kanpur, India. Dr. Shukla is also an Intermediate Fellow of the Wellcome Trust-DBT India Alliance. The research program in Dr. Shukla’s laboratory is focused on understanding the molecular mechanism of activation, signaling and regulation of G Protein-Coupled Receptors.

Affiliations and Expertise

Indian Institute of Technology, Kanpur, India