Description

Fundamentals of Advanced Omics Technologies: From Genes to Metabolites covers the fundamental aspects of the new instrumental and methodological developments in omics technologies, including those related to genomics, transcriptomics, epigenetics, proteomics and metabolomics, as well as other omics approaches such as glycomics, peptidomics and foodomics. The principal applications are presented in the following complementary volume.

The chapters discuss in detail omics technologies, DNA microarray analysis, next-generation sequencing technologies, genome-wide analysis of methylation and histone modifications, emerging nanotechniques in proteomics, imaging mass spectrometry in proteomics, recent quantitative proteomics approaches, and advances in high-resolution NMR-based metabolomics, as well as MS-based non-targeted metabolomics and metabolome analysis by CE-MS, global glycomics analyses, foodomics, and high resolution analytical tools for quantitative peptidomics. Key aspects related to chemometrics, bioinformatics, data treatment, data integration and systems biology, deep-sequencing data analysis, statistical approaches for the analysis of microarray data, the integration of transcriptome and metabolome data and computational approaches for visualization and integration of omics data are also covered.

Key Features

  • Covers the latest advances in instrumentation, experimental design, sample preparation, and data analysis
  • Provides thorough explanations and descriptions of specific omics technologies
  • Describes advanced tools and methodologies for data pretreatment, storage, curation and analysis, as well as data integration

Readership

Analytical scientists, PhD students and technicians in control laboratories, universities, hospitals, research laboratories and regulation agencies

Table of Contents

Contributors to Volume 63
Series Editor's Preface
Preface
Chapter 1: DNA Microarrays Technology: Overview and Current Status
Abstract

1 Introduction and Overview

2 Types of DNA Microarrays

3 Applications of Microarrays

4 Microarray Bioinformatics

5 Discussion and Concluding Remarks

Chapter 2: Challenges and Future Trends in DNA Microarray Analysis
Abstract

1 Introduction

2 Toward Microarray POC Devices

3 Validity of Microarray Data

4 Clinical Adoption

5 Future Trends of Microarray

6 Conclusion

Chapter 3: Next-Generation Sequencing: New Tools to Solve Old Challenges
Abstract

1 Introduction

2 Basis for NGS

3 Sample Preparation for NGS

4 Sequencing Techniques

5 NGS Data Analysis

6 Main Applications of NGS

7 Integrating Omics Data

Chapter 4: Omics Tools for the Genome-Wide Analysis of Methylation and Histone Modifications
Abstract

1 Omics Meets Epigenetics

2 Methods in Epigenomics

3 Concluding Remarks

Chapter 5: An Overview of Quantitative Proteomic Approaches
Abstract

1 Introduction

2 Immuno-Based Detection Methods

3 Mass Spectrometry-Based Detection Methods

4 Conclusions

Acknowledgments

Chapter 6: Emerging Nanotechniques in Proteomics
Abstract

1 Introduction

2 Overview of Protein Microarrays

3 Detection Platforms in Nanoproteomics

4 Biomarker Discovery by Nanoproteomics Approaches

5 Conclusions

Acknowledgments

Chapter 7: Mass Spectrometry Imaging in Proteomics and Metabolomics
Abstract

1 The Need for Imaging-Based Proteomics and Metabolomics

2 Mass Spectrometry Imaging

3 Applications of MSI

4 Future Developments

Chapter 8: Advances in NMR-Based Metabolomics
Abstract

1 Introduction

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Details

No. of pages:
490
Language:
English
Copyright:
© 2014
Published:
Imprint:
Elsevier
Electronic ISBN:
9780444626707
Print ISBN:
9780444626516