Description

Fundamentals of Advanced Omics Technologies: From Genes to Metabolites covers the fundamental aspects of the new instrumental and methodological developments in omics technologies, including those related to genomics, transcriptomics, epigenetics, proteomics and metabolomics, as well as other omics approaches such as glycomics, peptidomics and foodomics. The principal applications are presented in the following complementary volume.

The chapters discuss in detail omics technologies, DNA microarray analysis, next-generation sequencing technologies, genome-wide analysis of methylation and histone modifications, emerging nanotechniques in proteomics, imaging mass spectrometry in proteomics, recent quantitative proteomics approaches, and advances in high-resolution NMR-based metabolomics, as well as MS-based non-targeted metabolomics and metabolome analysis by CE-MS, global glycomics analyses, foodomics, and high resolution analytical tools for quantitative peptidomics. Key aspects related to chemometrics, bioinformatics, data treatment, data integration and systems biology, deep-sequencing data analysis, statistical approaches for the analysis of microarray data, the integration of transcriptome and metabolome data and computational approaches for visualization and integration of omics data are also covered.

Key Features

  • Covers the latest advances in instrumentation, experimental design, sample preparation, and data analysis
  • Provides thorough explanations and descriptions of specific omics technologies
  • Describes advanced tools and methodologies for data pretreatment, storage, curation and analysis, as well as data integration

Readership

Analytical scientists, PhD students and technicians in control laboratories, universities, hospitals, research laboratories and regulation agencies

Table of Contents

Contributors to Volume 63
Series Editor's Preface
Preface
Chapter 1: DNA Microarrays Technology: Overview and Current Status
Abstract

1 Introduction and Overview

2 Types of DNA Microarrays

3 Applications of Microarrays

4 Microarray Bioinformatics

5 Discussion and Concluding Remarks

Chapter 2: Challenges and Future Trends in DNA Microarray Analysis
Abstract

1 Introduction

2 Toward Microarray POC Devices

3 Validity of Microarray Data

4 Clinical Adoption

5 Future Trends of Microarray

6 Conclusion

Chapter 3: Next-Generation Sequencing: New Tools to Solve Old Challenges
Abstract

1 Introduction

2 Basis for NGS

3 Sample Preparation for NGS

4 Sequencing Techniques

5 NGS Data Analysis

6 Main Applications of NGS

7 Integrating Omics Data

Chapter 4: Omics Tools for the Genome-Wide Analysis of Methylation and Histone Modifications
Abstract

1 Omics Meets Epigenetics

2 Methods in Epigenomics

3 Concluding Remarks

Chapter 5: An Overview of Quantitative Proteomic Approaches
Abstract

1 Introduction

2 Immuno-Based Detection Methods

3 Mass Spectrometry-Based Detection Methods

4 Conclusions

Acknowledgments

Chapter 6: Emerging Nanotechniques in Proteomics
Abstract

1 Introduction

2 Overview of Protein Microarrays

3 Detection Platforms in Nanoproteomics

4 Biomarker Discovery by Nanoproteomics Approaches

5 Conclusions

Acknowledgments

Chapter 7: Mass Spectrometry Imaging in Proteomics and Metabolomics
Abstract

1 The Need for Imaging-Based Proteomics and Metabolomics

2 Mass Spectrometry Imaging

3 Applications of MSI

4 Future Developments

Chapter 8: Advances in NMR-Based Metabolomics
Abstract

1 Introduction

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Details

No. of pages:
490
Language:
English
Copyright:
© 2014
Published:
Imprint:
Elsevier
eBook ISBN:
9780444626707
Print ISBN:
9780444626516

About the series-volume-editors

Carolina Simó

Carolina Simó is a Tenured Researcher at the National Research Council of Spain (CSIC) in Madrid, she develops her scientific activities in the Laboratory of Foodomics. Her running research lines include: i) Method development using electrophoretic/chromatographic techniques coupled to mass spectrometry for Proteomics and Metabolomics. ii) Food safety assessment, quality control and other features related to food processing, storage, authenticity; iii) Study of the effect of diet on health/disease and the mechanisms of nutrients and bioactive food components at the molecular level. She has published over 50 SCI papers and 10 book chapters. Her h index is 20 and her works have received over 1000 citations (August-2013).

Affiliations and Expertise

Laboratory of Foodomics, CSIC, Madrid, Spain

Alejandro Cifuentes

Dr. Alejandro Cifuentes is a Full Research Professor at the National Research Council of Spain (CSIC) in Madrid and Head of the Laboratory of Foodomics. He has been Director of the Institute of Food Science Research and Deputy Director of the Institute of Industrial Fermentations, both belonging to CSIC. Currently he is Director of the Metabolomics Platform belonging to the International Excellence Campus UAM+CSIC. Alejandro's activity includes the development of advanced analytical methods (e.g., metabolomics, transcriptomics, proteomics) for foodomics, food quality and safety, as well as extraction and characterization of bioactive compounds from natural sources and their effect on human health. He holds different national and international awards, is member of the Editorial Board of 11 international journals (including J. Chromatogr. A, J. Pharmaceut. Biomed., J. Sep. Sci., Food Anal. Method., Int. J. Mol. Sci.) and Editor of TrAC-Trends in Analytical Chemistry, Electrophoresis and Current Opinion in Food Science (Foodomics Section). He has published more than 230 SCI papers, 20 books and book chapters and 8 patents. His h index is 56 (February 2016) and his works have received more than 10000 citations. Alejandro has given more than 150 invited lectures in different national and international meetings in Europe, Asia, Africa, America and Oceania. He has defined for the first time in a SCI journal the new discipline of Foodomics.

Affiliations and Expertise

National Research Council of Spain (CSIC), Madrid, Spain

Virginia García-Cañas

Virginia García-Cañas works in the Laboratory of Foodomics on the development of advanced analytical methods for: Foodomics, including Genomics and Transcriptomics; food quality; food safety; and Nutrigenomic studies. Her research covers: i) development of advanced DNA/RNA-based methods for food analysis (genetically-modified organisms, pathogens, etc.), and ii) Nutrigenomic study of the effect of dietary polyphenols on cancer. She has published over 45 SCI papers and 7 book chapters. Her h index is 19 and her works have received over 900 citations (August-2013).

Affiliations and Expertise

Laboratory of Foodomics, CSIC, Madrid, Spain