Functional Microbial Genomics

Functional Microbial Genomics

1st Edition - December 12, 2002

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  • Editors: Brendan Wren, Nick Dorrell
  • Hardcover ISBN: 9780125215336

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Description

Functional Microbial Genomics, edited by two leading experts in the field, provides the researcher with an up-to-date collection of articles on post-genome technologies central to studying the function of microorganisms.Since the release of the first complete genome sequence of a free-living organism in 1995, over 100 microbial genomes have been completely sequenced. The advent of new technologies for post-genomic analyses has allowed the rapid exploitation of this genome sequence information, heralding a golden era in microbial research. Functional Microbial Genomics provides in-depth accounts from scientists working with these new technologies explaining both the techniques and the ways in which they have been applied to the study of gene function in different microbial species.Methods in Microbiology is the most prestigious series devoted to techniques and methodology in the field. Established for over 30 years, Methods in Microbiology will continue to provide you with tried and tested, cutting edge protocols to directly benefit your research.

Key Features

* Bioinformatic approaches to genome sequence analysis
* Details the design, construction and applications of DNA microarrays
* Covers advances in bacterial proteome and metagenome analysis
* Describes functional genomic approaches for novel antibiotic target and vaccine candidate discovery
* Presents case studies where functional genomic analyses have revolutionized our understanding of model species

Readership

Micro and molecular biologists, geneticists, parasitologists, virologists, pharmacologists, biotechnologists

Table of Contents

  • GENOME SEQUENCE ANALYSIS
    Annotation of genomes
    From sequence to consequence: in silico hypothesis generation and testing
    The atlas visualisation of genome-wide information

    CONSTRUCTION OF DNA MICROARRAYS
    Microarray design for bacterial genomes
    Glass slides microarrays for bacterial genomes

    COMPARAIVE NUCLEIC ACID ANALYSIS
    Representational display analysis of Cdna and genome comparisons
    Application of DNA microarrays for comparative and evolutionary genomics
    Gene expression during host-pathogen interactions: approaches to bacterial mRNA extraction and labelling for microarray analysis
    High throughput in vivo screens: signature-tagged mutagenesis
    Further strategies for signature-tagged mutagenesis and the application of oligonucleotide microarrays for the quantitation of DNA-tagged strains

    PROTEOME ANALYSIS
    Advances in bacterial proteome analysis
    Discovery of protein-protein interaction using two-hybrid systems

    SECTION V APPLICATIONS OF MICROBIAL GENOMICS
    Cloning the metagenome: Culture-independent access to the diversity and functions of the uncultivated microbial world
    Reverse vaccinology: from genome to vaccine
    Microbial genomics for antibiotic target discovery

    CASE STUDIES - BACTERIA
    Helicobacter pylori functional genomics
    Streptomyces coelicolor A3(2): from genome sequence to function
    Functional analysis of the Bacillus subtilis genome

    SECTION VII CASE STUDIES - PARASITES
    Plasmodium falciparum DNA microarrays and interpretation of data
    Functional analysis of the Plasmodium falciparum genome using transfection
    Chromosome fragmentation as an approach to whole genome analysis in trypanosomes

Product details

  • No. of pages: 428
  • Language: English
  • Copyright: © Academic Press 2002
  • Published: December 12, 2002
  • Imprint: Academic Press
  • Hardcover ISBN: 9780125215336

About the Series Volume Editors

Brendan Wren

Affiliations and Expertise

London School of Hygiene & Tropical Medicine, U.K.

Nick Dorrell

Affiliations and Expertise

London School of Hygiene and Tropical Medicine, UK

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