Functional Genomics and Proteomics in the Clinical Neurosciences, Volume 158

1st Edition

Editors: Scott Hemby Sabine Bahn
Hardcover ISBN: 9780444518538
eBook ISBN: 9780080465630
Imprint: Elsevier Science
Published Date: 9th October 2006
Page Count: 402
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Table of Contents

List of Contributors

Foreword

Functional Genomics and Proteomics in the Clinical Neurosciences

Tissue preparation and banking

Introduction

Identifying subjects

Collection and harvesting tissue

Documenting

RNA integrity

Protein integrity

Conclusions

Functional genomic methodologies

Introduction

Input sources of RNA

Gene expression profiling: toward an informed choice

Level of sensitivity to detect the molecules of interest

Magnitude of expression-level changes in the brain

Minimum starting material for functional genomic analysis

Verification of expression-profiling analysis

Conventional methods of analyzing gene expression: Northern hybridization

qPCR

Serial analysis of gene expression (SAGE)

Massive parallel signature sequencing (MPSS)

Total analysis of gene expression (TOGA)

Sequencing by hybridization (SBH)

Microarray platforms

Analyzing massive datasets

Regional and single cell assessment

RNA amplification strategies: aRNA amplification

Additional considerations

Conclusions

Methods for proteomics in neuroscience

Introduction

Subcellular fractionation

Expression proteomics

Functional proteomics

Mass spectrometry

Protein arrays

Conclusion

Functional genomics and proteomics in the clinical neurosciences: data mining and bioinformatics

Introduction

Experimental methods

Data analysis

Statistical analysis and pattern classification

Microarray case study

Interpretation and validation

Reproducibility of microarray studies: concordance of current analysis methods

Introduction

The data analysis pipeline

Assessment of data quality

Performance comparison

Validation


Description

The purpose of this work is to familiarize neuroscientists with the available tools for proteome research and their relative abilities and limitations. To know the identities of the thousands of different proteins in a cell, and the modifications to these proteins, along with how the amounts of both of these change in different conditions would revolutionize biology and medicine. While important strides are being made towards achieving the goal of global mRNA analysis, mRNA is not the functional endpoint of gene expression and mRNA expression may not directly equate with protein expression. There are many potential applications for proteomics in neuroscience: determination of the neuro-proteome, comparative protein expression profiling, post-translational protein modification profiling and mapping protein-protein interactions, to name but a few. Functional Genomics and Proteomics in Clinical Neuroscience will comment on all of these applications, but with an emphasis on protein expression profiling. This book combines the basic methodology of genomics and proteomics with the current applications of such technologies in understanding psychiatric illnesses.

Key Features

  • Introduction of basic methodologies in genomics and proteomics and their integration in psychiatry
  • Development of the text in sections related to methods, application and future directions of these rapidly advancing technologies
  • Use of actual data to illustrate many principles of functional genomics and proteomics.
  • Introduction to bioinformatics and database management techniques

Readership

Neuroscientists, neurologists, geneticists.


Details

No. of pages:
402
Language:
English
Copyright:
© Elsevier Science 2006
Published:
Imprint:
Elsevier Science
eBook ISBN:
9780080465630
Hardcover ISBN:
9780444518538

About the Editors

Scott Hemby Editor

Sabine Bahn Editor