Functional Genomics and Proteomics in the Clinical Neurosciences

Functional Genomics and Proteomics in the Clinical Neurosciences

1st Edition - October 9, 2006

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  • Editors: Scott Hemby, Sabine Bahn
  • Hardcover ISBN: 9780444518538
  • eBook ISBN: 9780080465630

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The purpose of this work is to familiarize neuroscientists with the available tools for proteome research and their relative abilities and limitations. To know the identities of the thousands of different proteins in a cell, and the modifications to these proteins, along with how the amounts of both of these change in different conditions would revolutionize biology and medicine. While important strides are being made towards achieving the goal of global mRNA analysis, mRNA is not the functional endpoint of gene expression and mRNA expression may not directly equate with protein expression. There are many potential applications for proteomics in neuroscience: determination of the neuro-proteome, comparative protein expression profiling, post-translational protein modification profiling and mapping protein-protein interactions, to name but a few. Functional Genomics and Proteomics in Clinical Neuroscience will comment on all of these applications, but with an emphasis on protein expression profiling. This book combines the basic methodology of genomics and proteomics with the current applications of such technologies in understanding psychiatric illnesses.

Key Features

* Introduction of basic methodologies in genomics and proteomics and their integration in psychiatry
* Development of the text in sections related to methods, application and future directions of these rapidly advancing technologies
* Use of actual data to illustrate many principles of functional genomics and proteomics.
* Introduction to bioinformatics and database management techniques


Neuroscientists, neurologists, geneticists.

Table of Contents

  • List of Contributors


    Functional Genomics and Proteomics in the Clinical Neurosciences

    Tissue preparation and banking


    Identifying subjects

    Collection and harvesting tissue


    RNA integrity

    Protein integrity


    Functional genomic methodologies


    Input sources of RNA

    Gene expression profiling: toward an informed choice

    Level of sensitivity to detect the molecules of interest

    Magnitude of expression-level changes in the brain

    Minimum starting material for functional genomic analysis

    Verification of expression-profiling analysis

    Conventional methods of analyzing gene expression: Northern hybridization


    Serial analysis of gene expression (SAGE)

    Massive parallel signature sequencing (MPSS)

    Total analysis of gene expression (TOGA)

    Sequencing by hybridization (SBH)

    Microarray platforms

    Analyzing massive datasets

    Regional and single cell assessment

    RNA amplification strategies: aRNA amplification

    Additional considerations


    Methods for proteomics in neuroscience


    Subcellular fractionation

    Expression proteomics

    Functional proteomics

    Mass spectrometry

    Protein arrays


    Functional genomics and proteomics in the clinical neurosciences: data mining and bioinformatics


    Experimental methods

    Data analysis

    Statistical analysis and pattern classification

    Microarray case study

    Interpretation and validation

    Reproducibility of microarray studies: concordance of current analysis methods


    The data analysis pipeline

    Assessment of data quality

    Performance comparison


    Implications for data mining

    Summary and conclusions

    The genomics of mood disorders


    Genetics of mood disorders: the progress

    Neurobiological and neuroanatomical substrates of severe mood disorders

    The pathophysiology of severe mood disorders: insights from recent gene profiling studies

    Clues from animal models

    Concluding remarks

    Transcriptome alterations in schizophrenia: disturbing the functional architecture of the dorsolateral prefrontal cortex

    Dysfunction of the DLPFC in schizophrenia

    Types of transcriptome alterations in the DLPFC in schizophrenia

    Causes of transcriptome alterations in the DLPFC in schizophrenia

    Consequences of transcriptome alterations in the DLPFC in schizophrenia


    Strategies for improving sensitivity of gene expression profiling: regulation of apoptosis in the limbic lobe of schizophrenics and bipolars



    Assessment of genome and proteome profiles in cocaine abuse


    Neuroanatomy of cocaine addiction

    Functional genomics



    Neuronal gene expression profiling: uncovering the molecular biology of neurodegenerative disease


    Alzheimer's disease

    Determination of RNA within senile plaques and neurofibrillary tangles in AD

    Single cell gene array analysis of hippocampal senile plaques in AD

    Single cell gene analysis of hippocampal NFTs in AD

    Regional gene expression profiling in the hippocampus in AD

    Regional gene expression profiling in frontal and temporal neocortex in AD

    Regional gene expression profiling in other AD-related brain regions

    Single cell analysis of cholinergic basal forebrain (CBF) neurons in AD

    Single cell profiling of galanin hyperinnervated CBF neurons in AD

    Summary of gene expression profiling in AD

    Parkinson's disease

    Regional gene profiling of the substantia nigra in PD

    Gene expression profiling of Lewy body-containing SNpc neurons in PD

    Summary of gene expression profiling in PD


    Regional gene expression profiling in frontal cortex in schizophrenia

    Single cell gene profiling in the entorhinal cortex in schizophrenia

    Multiple sclerosis

    Gene profiling in multiple sclerosis

    Creutzfeld–Jakob disease

    Gene profiling in the aged brain

    Single cell profiling of aged CA1 and CA3 hippocampal neurons

    Gene regulation during the course of normal aging within the frontal cortex



    Epileptogenesis-related genes revisited



    Results and discussion

    Concluding remarks


    Functional genomics of sex hormone-dependent neuroendocrine systems: specific and generalized actions in the CNS

    Neural and genomic mechanisms for female mating behaviors

    From lordosis to sexual arousal to generalized CNS arousal

    From generalized CNS arousal to specific forms of arousal

    Molecular biology of histamine receptors in CNS

    α1B-Noradrenergic receptor signaling

    μ and δ opioid receptor signaling

    Summary and outlook


    Implications for the practice of psychiatry



    mRNA expression arrays (expressomics)

    Whole genome SNP association studies

    Use of convergent evidence

    Future directions

    Human brain evolution

    Anatomical evolution

    Protein sequence evolution

    Gene expression evolution

    Theory of gene expression evolution

    Adaptive human brain evolution


    Subject Index

    Erratum to Progress in Brain Research Vol. 158 Functional Genomics and Proteomics in the Clinical Neurosciences Scott E. Hemby and Sabine Bahn

Product details

  • No. of pages: 402
  • Language: English
  • Copyright: © Elsevier Science 2006
  • Published: October 9, 2006
  • Imprint: Elsevier Science
  • Hardcover ISBN: 9780444518538
  • eBook ISBN: 9780080465630

About the Editors

Scott Hemby

Sabine Bahn

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