From Metabolite, to Metabolism, to Metabolon

From Metabolite, to Metabolism, to Metabolon

1st Edition - April 1, 1992

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  • Editors: Earl R. Stadtman, P. Boon Chock
  • eBook ISBN: 9781483217321

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Description

Current Topics in Cellular Regulation, Volume 33: From Metabolite, to Metabolism, to Metabolon is a collection of articles on the study of the machinery of the living cell. The book is also a tribute to Paul Srere, an eminent biochemist, on the occasion of his sixty-fifth birthday. The book contains contributions written by students, postdoctoral fellows, and biochemists within Paul Srere's broad sphere of influence. The text provides articles that discuss topics on cellular infrastructure and metabolic organization; how carbohydrates cross the lipid membrane of bacterial cells; and macromolecular processes. The structural aspects of citrate biochemistry; the electron transport systems; nucleotide biosynthesis; enzyme organization and the direction of metabolic flow; and bioengineering applications are tackled as well. Biochemists, cytologists, and cell biologists will find the book very insightful.

Table of Contents


  • Paul A. Srere

    Section I. The Cytomatrix And Enzyme Organization

    Cellular Infrastructure and Metabolic Organization

    I. Introduction

    II. What Is the Cytosol?

    III. The Nature of the Cytomatrix: An Unresolved Issue

    IV. Protein Synthesis in Permeabilized Cells

    V. On the Ontogeny and Evolutionary Origin of the Cytomatrix

    References

    Association of Glycolytic Enzymes with the Cytoskeleton

    I. Introduction

    II. Dynamic Involvement of the Cytoskeleton in Intracellular Structure and Organization, Transportation, and Cellular Contraction

    III. Association of Glycolytic Enzymes with the Cytoskeleton

    IV. Summary

    References

    Interactions of Glycolytic Enzymes with Cellular Membranes

    I. Introduction

    II. Hexokinase

    III. Phosphofructokinase

    IV. Fructose- 1,6-Bisphosphate Aldolase

    V. Glyceraldehyde-3-Phosphate Dehydrogenase

    VI. Concluding Remarks

    References

    Section II. Membrane-Associated Processes

    How Carbohydrates Cross the Lipid Membrane of Bacterial Cells

    I. Introduction

    II. Binding Protein-Dependent Systems

    III. Cation Symport Systems

    IV. Phosphoenolpyruvate-Dependent Phosphotransferase System

    References

    Platelet-Activating Factor: A Novel Lipid Agonist

    I. Introduction

    II. Characterization of Platelet-Activating Factor Receptor(s) by Use of Antagonists

    III. Biological and Chemical Characteristics of Platelet-Activating Factor

    IV. Endogenous Modulators of Platelet-Activating Factor Activity

    V. Concluding Remarks

    References

    Section III. Macromolecular Processes

    Protein-Protein Interaction in the α-Complementation System of ß-Galactosidase

    I. Introduction

    II. Converting Full-Length Chimeric lacZ Genes to Chimeric lacZα Genes

    III. Demonstrating the Interaction of Chimeric α-Peptides with α-Acceptor via TrpR Phenotypes

    IV. Summary and Future Prospects in α-Complementation Research

    References

    Calmodulin and Dynamics of Interactions of Cytosolic Enzymes

    I. Introduction: Relationship of Filament Systems and Calmodulin

    II. Interactions in Muscle Protein Systems

    III. Interactions in Brain Protein Systems

    IV. Concluding Remarks

    Note Added in Proof

    References

    Chaperones and Matchmakers: Inhibitors and Stimulators of Protein Phosphorylation

    I. Introduction

    II. Model for the Action of Chaperones and Matchmakers

    III. Temperature Dependence of the Action of Chaperones

    IV. Role of Chaperones and Matchmakers as Substrate Modulators in Phosphorylation-Dephosphorylation Reactions

    References

    Organization of RNA Splicing in the Cell Nucleus

    I. Split Genes and Mammalian mRNA Processing

    II. Molecular Dissection of a Subnuclear RNA Splicing Domain

    III. Concluding Remarks

    References

    Section IV. Citric Acid Cycle

    Structural Aspects of Citrate Biochemistry

    I. Introduction

    II. Structure of Citrates

    III. Conclusion

    References

    Citrate: Its Relation to Free Magnesium Ion Concentration and Cellular Energy

    I. Introduction

    II. The Aconitase Reaction

    III. Adjustment of Keq for Variations in Temperature, Ionic Strength, and Free [Mg2+]

    IV. Determination of Free [Mg2+] from the Variation of the [Σ Citrate]/[Σ Isocitrate] Ratio

    V. Effects of Change of Free [Mg2+] on the Major Reactions of Energy Metabolism

    VI. Effects of Variation of Free [Mg2+] on Other Citrate Enzymes

    References

    Structure and Mechanism of Citrate Synthase

    I. Introduction

    II. General Background

    III. Enzyme-Substrate/Inhibitor Interactions

    IV. Implications for Catalytic Activity

    References

    Complexes and Complexities of the Citric Acid Cycle in Escherichia coli

    I. Introduction and Scope

    II. Gene-Protein Relationships and Expression

    III. Succinate Dehydrogenase, Fumarate Reductase, and Two Mechanisms of Oxygen-Regulated Gene Expression

    IV. Multiple Forms of Fumarase

    V. The 2-Oxo Acid Dehydrogenase Complexes

    VI. Concluding Remarks

    References

    Regulatory Consequences of Organization of Citric Acid Cycle Enzymes

    I. Introduction

    II. Materials and Methods

    III. Results

    IV. Discussion

    References

    The Molecular Physiology of Citrate

    I. Introduction

    II. Citrate Chemistry

    III. Citrate-Protein Interactions

    IV. Supramolecular Complexes

    V. Organellar and Higher Levels of Organization

    VI. Apologia

    References

    Section V. Electron Transport Systems

    The Protonmotive Force as an Intermediate in Electron Transport-Linked Phosphorylation: Problems and Prospects

    I. Introduction and Overview

    II. Principles of the Chemiosmotic Coupling Hypothesis

    III. Phosphorylation Driven by an Artificial Protonmotive Force

    IV. Values of the Protonmotive Force Obtained When Measured Directly

    V. Redox-Linked Proton Translocation

    VI. Coreconstituted Systems

    VII. Protoneural Proteins as Targets for Inhibitory Drugs

    VIII. Double-Inhibitor Titrations

    IX. What Types of Mechanism Are Therefore Possible?

    X. Nonlinear Dielectric Spectroscopy: A Novel Approach to the Study of Membrane Energy-Coupling Systems

    References

    Substrate Channeling of NADH in Mitochondrial Redox Processes

    I. Introduction

    II. NADH Channeling to Complex I

    Note Added in Proof

    References

    Interactions Between Mitochondria and Cytoplasm in Isolated Hepatocytes

    I. Introduction

    II. Materials

    III. Methods

    IV. Results and Discussion

    References

    Section VI. Nucleotide Biosynthesis

    Orotidylate Decarboxylase of Yeast and Man

    I. Introduction

    II. UMP Synthase, a Bifunctional Animal Protein Having Orotate Phosphoribosyltransferase and Orotidylate Decarboxylase Activities

    III. Mechanism of Reaction Catalyzed by Orotidylate Decarboxylase

    IV. Summary

    References

    Nucleoside Diphosphokinase: A Functional Link Between Intermediary Metabolism and Nucleic Acid Synthesis

    I. Metabolic Roles of Nucleoside Diphosphokinase

    II. Purification and Properties of Nucleoside Diphosphokinase from Escherichia coli

    III. Progress Toward Cloning and High-Level Expression of the Escherichia coli ndk Gene

    IV. Future Directions

    Note Added in Proof

    References

    Section VII. Theoretical Analysis Of Metabolism In Organized States

    Enzyme Organization and the Direction of Metabolic Flow: Physicochemical Considerations

    I. Enzyme Organization and Cellular Microenvironments

    II. Homogeneous Versus Heterogeneous Catalysis

    III. Diffusional Transit and Channeling

    IV. Free-Energy Transduction in Organized Enzyme Systems

    V. Quantification of Metabolic Control in Vivo

    VI. Concluding Remarks

    References

    Mechanisms of Metabolite Transfer Between Enzymes: Diffusional Versus Direct Transfer

    I. Introduction

    II. Dehydrogenases: Diffusional Versus Direct Transfer

    III. Two- and Three-Enzyme Pathways

    IV. Concluding Remarks

    References

    Section VIII. Bioengineering Applications

    Molecular Studies on Trypanothione Reductase: An Antiparasitic Target Enzyme

    I. Introduction: A Parasitic Analog of Glutathione

    II. Mechanistic Studies on Trypanothione Reductase

    III. Concluding Remarks

    References

    "Designer Membranes": Construction of a Cell Containing Multiple Membrane-Bound Cytochromes P450

    I. Introduction

    II. Cytochromes P450 and Their Role in Steroidogenesis

    III. Expression of Cytochromes P450 in COS Cells

    IV. Concluding Remarks

    References

    Index

Product details

  • No. of pages: 458
  • Language: English
  • Copyright: © Academic Press 1992
  • Published: April 1, 1992
  • Imprint: Academic Press
  • eBook ISBN: 9781483217321

About the Editors

Earl R. Stadtman

P. Boon Chock

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