From Gene to Protein: Translation into Biotechnology

From Gene to Protein: Translation into Biotechnology

1st Edition - January 1, 1982

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  • Editor: Fazal Ahmad
  • eBook ISBN: 9780323144612

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From Gene to Protein: Translation into Biotechnology is the 15th volume in the continuing series under the title ""Miami Winter Symposia"". The theme of the symposium is the translation of the basic research findings into the practical application of biotechnology. This book summarizes methodology and its applications that lie behind the practical innovations. The book starts with reviews of techniques of eukaryotic cell culture, hybridoma technology and uses, and the in vitro synthesis of DNA and its use in the generation of protein analogs. Considerable space is devoted to development of monoclonal antibodies that promises to be the dominating tool of medical technology, both for diagnosis and therapy. Cloning into eukaryotic cells and methods of increasing the levels of gene expression are included. These topics reflect areas of intensive research that have important commercial and clinical value. Core chapters describe biological activities of cloned gene products, including reports on trials with human subjects of interferon, human insulin, and growth hormone. A panel session on horizons in biotechnology is also provided, looking forward to the directions of future research and its applications. Biotechnologists, cell biologists, scientists, researchers, teachers, and students will greatly benefit from this book.

Table of Contents

  • Speakers and Discussants


    The Thirteenth Lynen Lecture

    Messing About with Isotopes and Enzymes and Antibodies


    Moving Genes: Promises Kept and Pending

    DISCUSSION: D. C. Burke, G. Haughton, G. F. Merrill, and C. Simonsen

    Techniques of Eukaryotic Cell Culture

    Cell Culture and Physiology

    Gordon H. Sato

    DISCUSSION: D. Balinsky, D. T. Denhardt, and G. Knapp

    Regulation of Growth and Differentiation of Mammalian Cells by Hormones and Extracellular Matrix

    DISCUSSION: H. Busch and R. G. Smith

    Microcarriers and the Problem of High Density Cell Culture

    DISCUSSION: D. H. Gelfand and G. H. Sato

    Crown Gall—Nature's Genetic Engineer

    DISCUSSION: J. R. Geiger and K. Sirotkin

    Monoclonal Antibodies—Production and Uses

    Monoclonal Antibodies: The Production of Tailor-Made Serological Reagents

    DISCUSSION: A. A. Ansari, N. L. Levy, P. A. Liberti, and W. I. Schaeffer

    Monoclonal Antibodies in the Analysis of the Molecular Basis for Human Genetic Diseases

    DISCUSSION: J. Harford, R. J. Hay, J. L. Strominger, and R. L. Ward

    Human Cell Surface Antigens Studied with Monoclonal Antibodies

    DISCUSSION: D. H. Gelfand

    Immunocytochemistry with Monoclonal Antibodies: Potential Applications in Basic Sciences and Histopathology

    Human Immunoglobulin Expression in Hybrid Cells

    DISCUSSION: S. I. Chavin, J. W. Larrick, and J. L. Stroming

    In Vitro Synthesis of DNA and the Generation of Protein Analogs

    The Role of Synthetic DNA in the Preparation of Structural Genes Coding for Proteins

    DISCUSSION: A. A. Ansari and U. R. Muller

    The Synthesis and Biochemical Reactivity of Biologically Important Genes and Gene Control Regions

    DISCUSSION: H. Heyneker, and unknown speaker

    Synthesis of Human Interferons and Analogs in Heterologous Cells

    DISCUSSION: M. Bina, D. Botstein, D. C. Burke, V. Chowdhry, M. Chretien, C. Colby, D. V. Hendrick, P. A. Liberti, U. R. Muller, S. A. Sherwin, S. L. C. Woo, H. Young, and J. B. Zeldis

    Cloning into Eukaryotic Cells

    Making Mutations in Vitro and Putting Them Back into Yeast

    DISCUSSION: D. Gelfand, D. Schlessinger, K. Sirotkin, and A. M. Skalka

    Expression of the Human Growth Hormone Gene Is Regulated in Mouse Cells

    DISCUSSION: R. Meagher, M. Nilsen-Hamilton, and M. Pater

    Transfer of the Mouse Metallothionein-I Gene into Cultured Cells and into Animals

    DISCUSSION: M. Bina, D. Botstein, H. Faber, and J. Mills

    Increasing Levels of Gene Expression

    Construction of Three Hybrid Promoters and Their Properties in Escherichia coli

    DISCUSSION: D. Botstein and D. Gelfand

    Biological Activities of Cloned Gene Products

    Regulation of Histone Gene Expression in Human Cells

    DISCUSSION: M. Adesnik, B. Nelkin, and P. Reczek

    Clinical Investigation of Partially Pure and Recombinant DNA-Derived Leukocyte Interferon in Human Cancer

    DISCUSSION: D. Burke, J. Cahill, V. Chowdhry, P. A. Libera, and H. Young

    The Use of Biosynthetic Human Insulin in Man


    The Biological Effectiveness of Pituitary-Derived and Biosynthetic Methionyl-hGH in Animals and Man

    Biological Activity of a Cloned Human Enzyme Urokinase

    DISCUSSION: H. Heyneker and unknown speaker

    Activity of Cloned Gene Products in Animal Systems

    DISCUSSION: L. Baumbach, D. Burke, D. Gelfand, and R. Ward

    Studies on Poliovirus Produced by Transfection with Cloned Viral cDNA

    DISCUSSION: D. Burke, E. Lamon, R. Samulski, and N. Stebbing

    Horizons in Biotechnology

    Friday Afternoon Panel Discussion

    Free Communications

    Genes for Cytochrome P-450 and Their Regulation

    Localization of a Heparin Binding Site of Fibronectin with the Aid of Monoclonal Antibodies

    Nucleotide Sequence on the gig C Gene Coding for ADP-Glucose Synthetase from E. coli

    Isolation and Characterization of a Soybean Gene for the Small Subunit of Ribulose-1,5-Bisphosphate Carboxylase

    Studying Polyoma Virus Gene Expression and Function by Site-Directed Mutagenesis Using Synthetic Oligonucleotides

    Characterization of Four Divergent Human Genomic Clones Homologous to the Transforming P21 Genes of Harvey and Kirsten Murine Sarcoma Viruses

    In Vitro Synthesis of Enzymes Involved in Membrane Biosynthesis in E. coli

    Studies on Expression of the Phaseolin Gene of French Bean Seeds in Sunflower Plant Cells

    DNA Stimulates ATP-Dependent Proteolysis and Protein-Dependent ATPase Activity of Protease La from Escherichia coli

    Methylation in Cellular Membranes of Friend Virus Tumors of Mice

    Identification of a Novel, H-2-Like Gene by Analysis of cDNA Clones

    A Genetic Analysis of the Glucocorticoid Response

    Nucleotide Sequence of the Region Surrounding the 5' End of 18S rRNA from Soybean Inferred from the Gene Sequence

    An ELISA Assay for Detecting Cell Surface Antigens on Adherent Cells

    The Involvement of DNA Polymerase α in Adenovirus DNA Replication

    Development of Human Growth Hormone Produced in Recombinant Bacteria as a Therapeutic Agent

    Expression of the Genes for the PST Restriction-Modification Enzymes after Cloning into a Temperature-Sensitive Replication Plasmid

    The Nucleotide Sequence of pACYC 177

    The Isolation of Sea Nettle Lethal Factor I via Immobilized Monoclonal Antibodies

    Synthesis of a Specific Sequence of DNA Using Restriction Enzymes

    Induction of Stress Proteins by Heat Shock and Metal Ions

    Analysis of the Rous Sacroma Virus Promoter Region by Site-Directed Mutagenesis

    Cloning and Sequencing of an Insulin-Like RNA from Human Brain

    Vasoactive Intestinal Polypeptide—From Gene to Protein to Peptide

    Detection of Specific Mouse Histone mRNAs by SI Nuclease Mapping

    Isolation and Characterization of Human Immune Interferon (IFN- γ) Messenger RNA

    Monoclonal Antibodies That Inhibit the Rat Liver Receptor for Asialoglycoproteins

    Solid Phase Nucleotide and Peptide Synthesis by Adaptation of Laboratory HPLC Equipment

    Cloning and Sequencing of a cDNA for αA Crystallin mRNA of the Mouse Lens: Presence of an Extensive 3' Untranslated Region

    Monoclonal Antibodies Recognizing Common Antigenic Determinants on Lysosomal Enzymes

    Proteins of a Miniplasmid Derivative of the Broad Host Range Plasmid RK2

    Monoclonal IgM Antibodies That Induce T Cell-Mediated Cytoxicity of Murine Sarcoma Cells

    Development of Methods for the Large-Scale Culture of Oxytricha nova

    Cloning of cDNA Encoding the Sweet-Tasting Plant Protein Thaumatin and Its Expression in Escherichia coli

    Amplification of the Metallothionein-I Gene in Cadmium- and Zinc-Resistant Chinese Hamster Ovary Cells

    Constitutive Thymidine Kinase Activity in Differentiating Mouse Myoblasts Transformed with Clone Herpes Virus Thymidine Kinase Genes

    Cloning of the Gene for α-Amylase from Bacillus stearothermophilus into Escherichia coli

    Expression of Foreign Genes in Recombinant Plasmids under the Control of T7 and T3 Late Promoters

    The Inability of in Vitro Transforming SV40 Subgenomes to Cause Tumors in Vivo Model Ribosomes

    Selective Induction by Peptide Growth Factors of the Synthesis of Secreted Proteins in 3T3 Cells

    Deletion of DNA Sequences between Preselected Bases

    Expression of Herpes Virus Thymidine Kinase Gene under Control of Early Promoter of SV40

    Regulation of Heavy Chain Gene Expression in Mouse Myeloma MOPC 315 Cells

    Amplification and Rapid Purification of Exonuclease I from Escherichia coli K-12

    Analysis of ß-Interferon mRNA in Human Cells

    The Purification of Microsomal of Glucose-6-Phosphatase from Human Liver: Aids to the Study Glycogen Storage Disease

    Transfer and Mapping of Genes Using Metaphase Chromosomes from ICR 2A Frog Cells

    Intracellular Selection for Plasmids That Carry Genes for Human Insulin

    Immunoelectron Microscopic Analysis of the Immunoglobulin Molecule for Idiotypes and Allotypes

    Cross Reactivity of Lethal Venoms to a Monoclonal Antibody

    Cloning of AAV pBR322: Rescue of Intact Virus from the Recombinant Plasmid in Human Cells

    Generation, Characterization, and Utilization of Monoclonal Antibodies Reactive with Human Mammary Carcinoma-Associated Antigens

    Structure of the Actin Multigene Family of Soybean (Glycine max)

    Identification of Defective Globin mRNA in Mel x Fibroblast Hybrid Cells

    A Plasmid Cloning Vector for Inducible Overproduction of Proteins in Bacterial Cells

    Characterization of Human Globin ψ ß2—A Phantom Pseudogene

    Production of Human Growth Hormone in Escherichia coli

    Novel Expression of an Interferon Gene in E. coli in High Yields

    HMG Proteins 1 and 2 Are Required for Transcription

    Tissue-Specific Methylation Patterns in Bovine Satellite DNA

    Foxomas: A Second-Generation Method for Obtaining Stable Heavy Chain Immunoglobulin Producing Hybridomas

    In Vitro and in Vivo Transcriptional Analysis of Mouse Globin Gene Promoters

    Purification of Plasmid DNAs and Synthetic Oligodeoxyribonucleotides Using Low-Pressure Anion-Exchange Chromatography

    Monoclonal Antibody against Ouabain Sensitivity-Related Human-Cell-Surface Antigen

    Selection of Monoclonal Antibodies for Designated Uses

    Toward Analysis of the HLA-DR System with Monoclonal Antibodies

    A Human Proinsulin cDNA Recombinant Clone

    Preparation of DNA Labeled with High Specific Activity 35S-Deoxyadenosine 5'-(α-Thio)Triphosphate: The Use of 35S-Labeled Nucleic Acids as Molecular Hybridization Probes

    DNA Transfer of Bacterial Asparagine Synthetase into Mammalian Cells

    Production and Characterization of Monoclonal Antibodies to a Calcium-Activated Protease Phenotypic Expression of Mammalian and Foreign Procaryotic Genes Cloned in Bacillus subtilis

    The Human α-1-Antitrypsin Gene and Pulmonary Emphysema

    Gene Expression during Mammalian Tooth Organ Development

    Index of Authors

    Subject Index

Product details

  • No. of pages: 612
  • Language: English
  • Copyright: © Academic Press 1982
  • Published: January 1, 1982
  • Imprint: Academic Press
  • eBook ISBN: 9780323144612

About the Editor

Fazal Ahmad

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