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From Gene to Protein: Translation into Biotechnology - 1st Edition - ISBN: 9780120455607, 9780323144612

From Gene to Protein: Translation into Biotechnology

1st Edition

Editor: Fazal Ahmad
eBook ISBN: 9780323144612
Imprint: Academic Press
Published Date: 1st January 1982
Page Count: 612
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From Gene to Protein: Translation into Biotechnology is the 15th volume in the continuing series under the title ""Miami Winter Symposia"". The theme of the symposium is the translation of the basic research findings into the practical application of biotechnology. This book summarizes methodology and its applications that lie behind the practical innovations. The book starts with reviews of techniques of eukaryotic cell culture, hybridoma technology and uses, and the in vitro synthesis of DNA and its use in the generation of protein analogs. Considerable space is devoted to development of monoclonal antibodies that promises to be the dominating tool of medical technology, both for diagnosis and therapy. Cloning into eukaryotic cells and methods of increasing the levels of gene expression are included. These topics reflect areas of intensive research that have important commercial and clinical value. Core chapters describe biological activities of cloned gene products, including reports on trials with human subjects of interferon, human insulin, and growth hormone. A panel session on horizons in biotechnology is also provided, looking forward to the directions of future research and its applications. Biotechnologists, cell biologists, scientists, researchers, teachers, and students will greatly benefit from this book.

Table of Contents

Speakers and Discussants


The Thirteenth Lynen Lecture

Messing About with Isotopes and Enzymes and Antibodies


Moving Genes: Promises Kept and Pending

DISCUSSION: D. C. Burke, G. Haughton, G. F. Merrill, and C. Simonsen

Techniques of Eukaryotic Cell Culture

Cell Culture and Physiology

Gordon H. Sato

DISCUSSION: D. Balinsky, D. T. Denhardt, and G. Knapp

Regulation of Growth and Differentiation of Mammalian Cells by Hormones and Extracellular Matrix

DISCUSSION: H. Busch and R. G. Smith

Microcarriers and the Problem of High Density Cell Culture

DISCUSSION: D. H. Gelfand and G. H. Sato

Crown Gall—Nature's Genetic Engineer

DISCUSSION: J. R. Geiger and K. Sirotkin

Monoclonal Antibodies—Production and Uses

Monoclonal Antibodies: The Production of Tailor-Made Serological Reagents

DISCUSSION: A. A. Ansari, N. L. Levy, P. A. Liberti, and W. I. Schaeffer

Monoclonal Antibodies in the Analysis of the Molecular Basis for Human Genetic Diseases

DISCUSSION: J. Harford, R. J. Hay, J. L. Strominger, and R. L. Ward

Human Cell Surface Antigens Studied with Monoclonal Antibodies


Immunocytochemistry with Monoclonal Antibodies: Potential Applications in Basic Sciences and Histopathology

Human Immunoglobulin Expression in Hybrid Cells

DISCUSSION: S. I. Chavin, J. W. Larrick, and J. L. Stroming

In Vitro Synthesis of DNA and the Generation of Protein Analogs

The Role of Synthetic DNA in the Preparation of Structural Genes Coding for Proteins

DISCUSSION: A. A. Ansari and U. R. Muller

The Synthesis and Biochemical Reactivity of Biologically Important Genes and Gene Control Regions

DISCUSSION: H. Heyneker, and unknown speaker

Synthesis of Human Interferons and Analogs in Heterologous Cells

DISCUSSION: M. Bina, D. Botstein, D. C. Burke, V. Chowdhry, M. Chretien, C. Colby, D. V. Hendrick, P. A. Liberti, U. R. Muller, S. A. Sherwin, S. L. C. Woo, H. Young, and J. B. Zeldis

Cloning into Eukaryotic Cells

Making Mutations in Vitro and Putting Them Back into Yeast

DISCUSSION: D. Gelfand, D. Schlessinger, K. Sirotkin, and A. M. Skalka

Expression of the Human Growth Hormone Gene Is Regulated in Mouse Cells

DISCUSSION: R. Meagher, M. Nilsen-Hamilton, and M. Pater

Transfer of the Mouse Metallothionein-I Gene into Cultured Cells and into Animals

DISCUSSION: M. Bina, D. Botstein, H. Faber, and J. Mills

Increasing Levels of Gene Expression

Construction of Three Hybrid Promoters and Their Properties in Escherichia coli

DISCUSSION: D. Botstein and D. Gelfand

Biological Activities of Cloned Gene Products

Regulation of Histone Gene Expression in Human Cells

DISCUSSION: M. Adesnik, B. Nelkin, and P. Reczek

Clinical Investigation of Partially Pure and Recombinant DNA-Derived Leukocyte Interferon in Human Cancer

DISCUSSION: D. Burke, J. Cahill, V. Chowdhry, P. A. Libera, and H. Young

The Use of Biosynthetic Human Insulin in Man


The Biological Effectiveness of Pituitary-Derived and Biosynthetic Methionyl-hGH in Animals and Man

Biological Activity of a Cloned Human Enzyme Urokinase

DISCUSSION: H. Heyneker and unknown speaker

Activity of Cloned Gene Products in Animal Systems

DISCUSSION: L. Baumbach, D. Burke, D. Gelfand, and R. Ward

Studies on Poliovirus Produced by Transfection with Cloned Viral cDNA

DISCUSSION: D. Burke, E. Lamon, R. Samulski, and N. Stebbing

Horizons in Biotechnology

Friday Afternoon Panel Discussion

Free Communications

Genes for Cytochrome P-450 and Their Regulation

Localization of a Heparin Binding Site of Fibronectin with the Aid of Monoclonal Antibodies

Nucleotide Sequence on the gig C Gene Coding for ADP-Glucose Synthetase from E. coli

Isolation and Characterization of a Soybean Gene for the Small Subunit of Ribulose-1,5-Bisphosphate Carboxylase

Studying Polyoma Virus Gene Expression and Function by Site-Directed Mutagenesis Using Synthetic Oligonucleotides

Characterization of Four Divergent Human Genomic Clones Homologous to the Transforming P21 Genes of Harvey and Kirsten Murine Sarcoma Viruses

In Vitro Synthesis of Enzymes Involved in Membrane Biosynthesis in E. coli

Studies on Expression of the Phaseolin Gene of French Bean Seeds in Sunflower Plant Cells

DNA Stimulates ATP-Dependent Proteolysis and Protein-Dependent ATPase Activity of Protease La from Escherichia coli

Methylation in Cellular Membranes of Friend Virus Tumors of Mice

Identification of a Novel, H-2-Like Gene by Analysis of cDNA Clones

A Genetic Analysis of the Glucocorticoid Response

Nucleotide Sequence of the Region Surrounding the 5' End of 18S rRNA from Soybean Inferred from the Gene Sequence

An ELISA Assay for Detecting Cell Surface Antigens on Adherent Cells

The Involvement of DNA Polymerase α in Adenovirus DNA Replication

Development of Human Growth Hormone Produced in Recombinant Bacteria as a Therapeutic Agent

Expression of the Genes for the PST Restriction-Modification Enzymes after Cloning into a Temperature-Sensitive Replication Plasmid

The Nucleotide Sequence of pACYC 177

The Isolation of Sea Nettle Lethal Factor I via Immobilized Monoclonal Antibodies

Synthesis of a Specific Sequence of DNA Using Restriction Enzymes

Induction of Stress Proteins by Heat Shock and Metal Ions

Analysis of the Rous Sacroma Virus Promoter Region by Site-Directed Mutagenesis

Cloning and Sequencing of an Insulin-Like RNA from Human Brain

Vasoactive Intestinal Polypeptide—From Gene to Protein to Peptide

Detection of Specific Mouse Histone mRNAs by SI Nuclease Mapping

Isolation and Characterization of Human Immune Interferon (IFN- γ) Messenger RNA

Monoclonal Antibodies That Inhibit the Rat Liver Receptor for Asialoglycoproteins

Solid Phase Nucleotide and Peptide Synthesis by Adaptation of Laboratory HPLC Equipment

Cloning and Sequencing of a cDNA for αA Crystallin mRNA of the Mouse Lens: Presence of an Extensive 3' Untranslated Region

Monoclonal Antibodies Recognizing Common Antigenic Determinants on Lysosomal Enzymes

Proteins of a Miniplasmid Derivative of the Broad Host Range Plasmid RK2

Monoclonal IgM Antibodies That Induce T Cell-Mediated Cytoxicity of Murine Sarcoma Cells

Development of Methods for the Large-Scale Culture of Oxytricha nova

Cloning of cDNA Encoding the Sweet-Tasting Plant Protein Thaumatin and Its Expression in Escherichia coli

Amplification of the Metallothionein-I Gene in Cadmium- and Zinc-Resistant Chinese Hamster Ovary Cells

Constitutive Thymidine Kinase Activity in Differentiating Mouse Myoblasts Transformed with Clone Herpes Virus Thymidine Kinase Genes

Cloning of the Gene for α-Amylase from Bacillus stearothermophilus into Escherichia coli

Expression of Foreign Genes in Recombinant Plasmids under the Control of T7 and T3 Late Promoters

The Inability of in Vitro Transforming SV40 Subgenomes to Cause Tumors in Vivo Model Ribosomes

Selective Induction by Peptide Growth Factors of the Synthesis of Secreted Proteins in 3T3 Cells

Deletion of DNA Sequences between Preselected Bases

Expression of Herpes Virus Thymidine Kinase Gene under Control of Early Promoter of SV40

Regulation of Heavy Chain Gene Expression in Mouse Myeloma MOPC 315 Cells

Amplification and Rapid Purification of Exonuclease I from Escherichia coli K-12

Analysis of ß-Interferon mRNA in Human Cells

The Purification of Microsomal of Glucose-6-Phosphatase from Human Liver: Aids to the Study Glycogen Storage Disease

Transfer and Mapping of Genes Using Metaphase Chromosomes from ICR 2A Frog Cells

Intracellular Selection for Plasmids That Carry Genes for Human Insulin

Immunoelectron Microscopic Analysis of the Immunoglobulin Molecule for Idiotypes and Allotypes

Cross Reactivity of Lethal Venoms to a Monoclonal Antibody

Cloning of AAV pBR322: Rescue of Intact Virus from the Recombinant Plasmid in Human Cells

Generation, Characterization, and Utilization of Monoclonal Antibodies Reactive with Human Mammary Carcinoma-Associated Antigens

Structure of the Actin Multigene Family of Soybean (Glycine max)

Identification of Defective Globin mRNA in Mel x Fibroblast Hybrid Cells

A Plasmid Cloning Vector for Inducible Overproduction of Proteins in Bacterial Cells

Characterization of Human Globin ψ ß2—A Phantom Pseudogene

Production of Human Growth Hormone in Escherichia coli

Novel Expression of an Interferon Gene in E. coli in High Yields

HMG Proteins 1 and 2 Are Required for Transcription

Tissue-Specific Methylation Patterns in Bovine Satellite DNA

Foxomas: A Second-Generation Method for Obtaining Stable Heavy Chain Immunoglobulin Producing Hybridomas

In Vitro and in Vivo Transcriptional Analysis of Mouse Globin Gene Promoters

Purification of Plasmid DNAs and Synthetic Oligodeoxyribonucleotides Using Low-Pressure Anion-Exchange Chromatography

Monoclonal Antibody against Ouabain Sensitivity-Related Human-Cell-Surface Antigen

Selection of Monoclonal Antibodies for Designated Uses

Toward Analysis of the HLA-DR System with Monoclonal Antibodies

A Human Proinsulin cDNA Recombinant Clone

Preparation of DNA Labeled with High Specific Activity 35S-Deoxyadenosine 5'-(α-Thio)Triphosphate: The Use of 35S-Labeled Nucleic Acids as Molecular Hybridization Probes

DNA Transfer of Bacterial Asparagine Synthetase into Mammalian Cells

Production and Characterization of Monoclonal Antibodies to a Calcium-Activated Protease Phenotypic Expression of Mammalian and Foreign Procaryotic Genes Cloned in Bacillus subtilis

The Human α-1-Antitrypsin Gene and Pulmonary Emphysema

Gene Expression during Mammalian Tooth Organ Development

Index of Authors

Subject Index


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© Academic Press 1982
1st January 1982
Academic Press
eBook ISBN:

About the Editor

Fazal Ahmad

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