Description

DNA typing has revolutionized criminal investigations and has become a powerful tool in the identification of individuals in criminal and paternity cases. Forensic DNA Biology: A Laboratory Manual is comprised of up-to-date and practical experiments and step-by-step instructions on how to perform DNA analysis, including pipetting, microscopy and hair analysis, presumptive testing of body fluids and human DNA typing. Modern DNA typing techniques are provided, reflecting real life, where not all institutions and crime labs can afford the same equipment and software. Real case studies will be used throughout and the author will provide an Instructor Manual and PowerPoint lecture slides.

Key Features

  • Provides practical step-by-step instruction on how to perform forensic DNA analysis
  • Includes analysis of hair, presumptive testing of body fluids, human DNA typing and statistics
  • Covers techniques such as pipetting, microscopy and DNA extraction
  • Pre- and post-lab exercises and questions assist the reader in learning the material
  • Report writing templates assure the reader learns real world crime lab procedure

Readership

Upper-level, undergraduate or graduate students majoring in forensic science, chemistry or biology, academia and newly practicing forensic scientists

Table of Contents

Acknowledgements

About the Author

Welcome

Forensic DNA Biology: An Introduction

Biology Overview

Restriction Fragment Length Polymorphisms

Polymerase Chain Reaction

Short Tandem Repeats

Single Nucleotide Polymorphisms

Mitochondrial DNA

Known versus Questioned Samples

Why Study Forensic DNA Biology?

Laboratory Safety

Rules for a Safe Lab Environment

Avoiding Contamination Issues

Chapter 1. Pipetting

Objective

Safety

Materials

Background

Procedure

Questions

Graphing the Data Using Microsoft Excel (2003)

Equations

REFERENCES

Chapter 2. Serology

Objective

Safety

Materials

Background

Procedure

Questions

REFERENCES

Chapter 3. Sampling Biological Evidence for DNA Extraction

Objective

Safety

Materials

Background

Procedure: DNA Collection and Packaging

Questions

REFERENCES

Chapter 4. DNA Extraction

Objective

Safety

Materials

Recipes for Buffer and Solution Preparation

Background

Procedure

Question

REFERENCES

Chapter 5. Determination of Quality and Quantity of DNA Using Agarose Gel Electrophoresis

Objective

Safety

Materials

Background

Procedure

Questions

REFERENCE

Chapter 6. Determination of DNA Quality and Quantity Using UV-Vis Spectroscopy

Objective

Safety

Materials

Background

Procedure

Questions

REFERENCES

Chapter 7. Determination of DNA Quantity by Fluorescence Spectroscopy

Objective

Safety

Materials

Background

Procedure

Questions

REFERENCES

Chapter 8. Real-Time Polymerase Chain Reactio

Details

No. of pages:
224
Language:
English
Copyright:
© 2013
Published:
Imprint:
Academic Press
Electronic ISBN:
9780123948335
Print ISBN:
9780123945853

About the author

Kelly Elkins

Dr. Elkins is Assistant Professor of Chemistry at Towson University where she teaches in the Masters of Science in Forensic Chemistry Program. Previously, she was the Director of Forensic Science and Assistant Professor of Chemistry at Metropolitan State College of Denver. She has her Bachelor of Science in Biology, Bachelor of Arts in Chemistry and her PhD in Chemistry with an emphasis on Biochemistry. Focused on the Forensic DNA field, she has developed a network of contacts in crime laboratories throughout Colorado and the Four Corners region and looks forward to extending her network in the Baltimore/DC area.

Reviews

"This manual presents a collection of novel laboratory experiments intended for advanced undergraduate students and those new to the field of forensic DNA biology and DNA typing." --Evidence Technology Magazine, July-August 2013