Description

This new edition of Fluorescent Proteins presents current applications of autofluorescent proteins in cell and molecular biology authored by researchers from many of the key laboratories in the field. Starting from a current review of the broad palette of fluorescent proteins available, several chapters focus on key autofluorescent protein variants, including spectral variants, photodynamic variants as well as chimeric FP approaches. Molecular applications are addressed in chapters that detail work with single molecules, approaches to generating protein fusions and biosensors as well as analysis of protein-protein interactions in vivo by FRET, fluorescence polarization and fluorescence cross correlation techniques. A number of approaches to in vivo dynamics are presented, including FRAP, photoactivation, and 4-dimensional microscopy. Behavior of spindle components, membrane proteins, mRNA trafficking as well as analysis of cell types in tissues and in development are detailed and provide models for a wide variety of experimental approaches. In addition, several chapters deal directly with the computational issues involved in processing multidimensional image data and using fluorescent imaging to probe cellular behavior with quantitative modeling. This volume brings together the latest perspective and techniques on fluorescent proteins and will be an invaluable reference in a wide range of laboratories.

Readership

Graduate students in basic research, scientists, technicians, postdoctoral, and experienced researchers in fields of cell and developmental biology, molecular biology, genetics, neuroscience, and biochemistry--anyone who examines cells in a microscope.

Table of Contents

1. Fluorescent Proteins Ian M. Dobie, Noel F. Lowndes and Kevin F. Sullivan 2. Functional Fusion Proteins by Random Transposon-Based GFP Insertion Robert Mealer, Heather Butler & Thomas Hughes 3. Fluorescent Proteins for Photoactivation Experiments Jennifer Lippincott-Schwartz and George H. Patterson 4. Design and Optimization of Genetically Encoded Fluorescent Biosensors: Gtpase Biosensors Louis Hodgson and Klaus M. Hahn 5. Fast 4D Microscopy J. R. De Mey, P. Kessler, J. Dompierre, F. P. Cordelières, A. Dieterlen, J. -L Vonesch, and J. –B. Sibarita 6. Single molecule imaging of fluorescent proteins Adam D. Douglass and Ronald D. Vale 7. Counting Kinetochore Protein Numbers in Budding Yeast Using Genetically Encoded Fluorescent Proteins Ajit Joglekar, E. D. Salmon, Kerry Bloom 8. Fluorescent Protein Applications in Plants R. Howard Berg, Roger N. Beachy 9. Expression and Imaging of Fluorescent Proteins in the C. elegans Gonad and Early Embryo Rebecca A. Green, Anjon Audhya, Andrei Pozniakovsky, Alexander Dammermann, Hayley Pemble, Joost Monen, Nathan Portier, Anthony Hyman, Arshad Desai and Karen Oegema 10. Fluorescent Proteins in Zebrafish Cell and Developmental Biology H. William Detrich, III 11. Identifying and Quantitating Neural Stem and Progenitor Cells in the Adult Brain Juan Manuel Encinas and Grigori Enikolopov 12. Using Fluorescent Proteins to Study mRNA Trafficking in Living Cells Emmanuelle Querido and Pascal Chartrand 13. Visualising mRNA Localisation and Local Protein Translation in Neurons<

Details

No. of pages:
660
Language:
English
Copyright:
© 2008
Published:
Imprint:
Academic Press
eBook ISBN:
9780080557243
Print ISBN:
9780123725585

About the editor

Kevin Sullivan

Affiliations and Expertise

Department of Biochemistry, National University of Ireland, Galway, Ireland