Fluorescence Spectroscopy

Fluorescence Spectroscopy

1st Edition - December 11, 2008
This is the Latest Edition
  • Editors: Ludwig Brand, Michael Johnson
  • Hardcover ISBN: 9780123745866
  • eBook ISBN: 9780080923345

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Description

Fluorescence spectroscopy is a type of electromagnetic spectroscopy, using a beam of light, which analyzes fluorescence from a sample. Given its extremely high sensitivity and selectivity, it is an important investigational tool in many areas including material sciences, analytical sciences, and across a broad range of chemical, biochemical and medical research. It has become an essential investigational technique allowing detailed, real-time observation of the structure and dynamics of intact biological systems. The pharmaceutical industry uses it heavily and it has become a dominating technique in biochemistry and molecular genetics.

Key Features

* Keeps MIE buyers and online subscribers up-to-date with the latest research with this highly used technique.

* Provides tried and tested techniques which eliminate searching through many different sources.

Readership

Biochemists, biophysicists, cell biologists, and molecular biologists.

Table of Contents

  • Direct quantitation of Mg2+ - RNA interactions use of a fluorescent dye
    Dan Grilley, Ana Maria Soto, and David E. Draper

    A Method in Enzymology for Measuring Intracellular Protease Activities in Live Cells
    Beverly Z. Packard and Akira Komoriya

    Using Fluorophore-labeled Oligonucleotides to Measure Affinities of Protein-DNA Interactions
    Brian J. Anderson and Joel F. Schildbach

    Site-Selective Red-Edge Effects
    Alexander P. Demchenko

    Multiparametric Probing of Microenvironment with Solvatochromic Fluorescent Dyes Andrey S. Klymchenko and Alexander Demchenko

    Pteridine Probes for Nucleic Acid Analysis
    Mary E. Hawkins

    Application of Single Molecule Spectroscopy in Studying Enzyme Kinetics and Mechanism Jue Shi, Ari Gafni, and Duncan Steel


    Fluorescence Approaches in Quantifying Bio-Molecular Interactions
    Catherine A. Royer and Suzanne

    Applications of Up-Conversion Methods in Studies of Proteins and Peptides
    Jay Knutson

    Spectroscopic Studies of Molecular Solvent Relaxation
    Mark Maroncelli

    Single Molecule Fluorescence Studies of the Dynamics of Gene Regulation
    Jie Xiao

    New Fluorescence Methods for Studies of Muscle Proteins
    Robert E. Dale

    Fluorescence Probes for Analysis of Membrane Proteins
    Amitabha Chattopadhyay

    Fluorescence Methods for Protein-Protein interaction in Membranes
    Kalina Hristova

    Fluorescence Detection of Specific Ions
    Bernard Valeur

    Measuring Picomolar Intracellular Exchangable Zn in PC-12 Cells using a Ratiometric Fluorescence Biosensor
    R.A. Bozym

    Monitoring Free Zinc in the Mitochondrion
    Richard B Thompson, et al

    Metal-enhanced fluorescence: An emerging tool in biotechnology
    Chris D. Geddes, et al

    Radiative Decay Engineering, Surface Plasmon-Coupled Directional Emission
    Joseph R Lakowicz, et al

    Time-resolved fluorescence anisotropy and the measurement of protein conformational flexibility
    David A. Johnson (University of California Riverside)

    Tranes and other new Analysis Procedures in wavelength resolved fluorescence.
    Pariasamy (Mumbay)

    From Quantum Theory to Molecular Fluorescence.
    D. Toptygin

    Probing Protein Electrostatics with Synthetic amino acids.
    Bruce Cohen (UCSF)

    Analysis of Complex Fluorescence Anisotropy Decay
    William Laws, Montana

    Studies of Segmental Protein Dynamics with Time and Energy ? Resolved Fluorescence Spectroscopy
    D. Toptygin and L. Brand

    Complex Fluorescence Decay of Trytophan in Proteins.
    Mary D. Barkley

Product details

  • No. of pages: 400
  • Language: English
  • Copyright: © Academic Press 2008
  • Published: December 11, 2008
  • Imprint: Academic Press
  • Hardcover ISBN: 9780123745866
  • eBook ISBN: 9780080923345

About the Serial Volume Editors

Ludwig Brand

Affiliations and Expertise

McCollum-Pratt Institute, Johns Hopkins University, Baltimore, MD, USA

Michael Johnson

Affiliations and Expertise

University of Virginia Health Sciences Center, Charlottesville, USA