Fluorescence spectroscopy is a type of electromagnetic spectroscopy, using a beam of light, which analyzes fluorescence from a sample. Given its extremely high sensitivity and selectivity, it is an important investigational tool in many areas including material sciences, analytical sciences, and across a broad range of chemical, biochemical and medical research. It has become an essential investigational technique allowing detailed, real-time observation of the structure and dynamics of intact biological systems. The pharmaceutical industry uses it heavily and it has become a dominating technique in biochemistry and molecular genetics.

Key Features

* Keeps MIE buyers and online subscribers up-to-date with the latest research with this highly used technique. * Provides tried and tested techniques which eliminate searching through many different sources.


Biochemists, biophysicists, cell biologists, and molecular biologists.

Table of Contents

Direct quantitation of Mg2+ - RNA interactions use of a fluorescent dye Dan Grilley, Ana Maria Soto, and David E. Draper A Method in Enzymology for Measuring Intracellular Protease Activities in Live Cells Beverly Z. Packard and Akira Komoriya Using Fluorophore-labeled Oligonucleotides to Measure Affinities of Protein-DNA Interactions Brian J. Anderson and Joel F. Schildbach Site-Selective Red-Edge Effects Alexander P. Demchenko Multiparametric Probing of Microenvironment with Solvatochromic Fluorescent Dyes Andrey S. Klymchenko and Alexander Demchenko Pteridine Probes for Nucleic Acid Analysis Mary E. Hawkins Application of Single Molecule Spectroscopy in Studying Enzyme Kinetics and Mechanism Jue Shi, Ari Gafni, and Duncan Steel Fluorescence Approaches in Quantifying Bio-Molecular Interactions Catherine A. Royer and Suzanne Applications of Up-Conversion Methods in Studies of Proteins and Peptides Jay Knutson Spectroscopic Studies of Molecular Solvent Relaxation Mark Maroncelli Single Molecule Fluorescence Studies of the Dynamics of Gene Regulation Jie Xiao New Fluorescence Methods for Studies of Muscle Proteins Robert E. Dale Fluorescence Probes for Analysis of Membrane Proteins Amitabha Chattopadhyay Fluorescence Methods for Protein-Protein interaction in Membranes Kalina Hristova Fluorescence Detection of Specific Ions Bernard Valeur Measuring Picomolar Intracellular Excha


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© 2008
Academic Press
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About the serial-volume-editors

Ludwig Brand

Affiliations and Expertise

McCollum-Pratt Institute, Johns Hopkins University, Baltimore, MD, USA

Michael Johnson

Affiliations and Expertise

University of Virginia Health Sciences Center, Charlottesville, USA