Fluorescence Fluctuation Spectroscopy (FFS) Part B

Fluorescence Fluctuation Spectroscopy (FFS) Part B

1st Edition - December 29, 2012
This is the Latest Edition
  • Editor: Sergey Tetin
  • Hardcover ISBN: 9780124055391
  • eBook ISBN: 9780124058576

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Description

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers fluorescence fluctuation spectroscopy and includes chapters on such topics as Förster resonance energy transfer (fret) with fluctuation algorithms, protein corona on nanoparticles by FCS, and FFS approaches to the study of receptors in live cells.

Key Features

  • Continues the legacy of this premier serial with quality chapters authored by leaders in the field
  • Covers fluorescence fluctuation spectroscopy
  • Contains chapters on such topics as Förster resonance energy transfer (fret) with fluctuation algorithms, protein corona on nanoparticles by FCS, and FFS approaches to the study of receptors in live cells

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of Contents

  • Contributors

    Preface

    Methods in Enzymology

    Chapter One. FCS in STED Microscopy

    1 Introduction

    2 STED-FCS of Lipid Membrane Dynamics

    3 Lipid Membrane Dynamics

    4 Conclusions

    References

    Further Reading

    Chapter Two. Analyzing Förster Resonance Energy Transfer with Fluctuation Algorithms

    1 Introduction

    2 FRET and FCS

    3 Filtered FCS

    4 Applications

    5 Discussion

    References

    Chapter Three. Fluorescence Fluctuation Spectroscopy Approaches to the Study of Receptors in Live Cells

    1 Introduction

    2 Selected FFS Studies

    3 Choice of Fluorophores: General Considerations

    4 Cells: General Considerations

    5 Summary

    References

    Chapter Four. Studying the Protein Corona on Nanoparticles by FCS

    1 Introduction

    2 Sample Preparation

    3 Experimental Procedures

    4 Data Analysis

    5 Protein Corona Formation Measured by FCS

    6 Conclusions

    References

    Chapter Five. Studying Antibody–Antigen Interactions with Fluorescence Fluctuation Spectroscopy

    1 Introduction

    2 Binding Model and Experimental Considerations

    3 Studying Antibodies with FFS

    4 Instrumentation

    References

    Chapter Six. Fluorescence Fluctuation Approaches to the Study of Adhesion and Signaling

    1 Introduction

    2 A Fluorescence Fluctuation Toolbox

    3 Experimental Implementation

    4 Applications

    5 Conclusion

    References

    Chapter Seven. Interactions in Gene Expression Networks Studied by Two-Photon Fluorescence Fluctuation Spectroscopy

    1 Introduction

    2 In Vitro Interactions Between Proteins and Nucleic Acids Using Fluctuation Approaches

    3 FFM in Live Bacterial Cells

    4 Conclusions and Perspectives

    References

    Chapter Eight. Studying Ion Exchange in Solution and at Biological Membranes by FCS

    1 Introduction

    2 Ion Exchange Monitoring by FCS—Basic Approach

    3 Monitoring of Local Ion Concentrations and Exchange in Solution

    4 Monitoring of Proton Exchange at Biological Membranes by FCS

    5 Approach for Ion Exchange Monitoring Incorporating Dual Color Fluorescence Cross Correlation Spectroscopy (FCCS)

    6 Conclusions

    References

    Chapter Nine. Fluctuation Analysis of Activity Biosensor Images for the Study of Information Flow in Signaling Pathways

    1 Introduction

    2 Activity Biosensors

    3 Extracting Activity Fluctuations in a Cell Shape Invariant Space

    4 Correlation Analysis of Activity Fluctuations for Pathway Reconstruction

    5 Outlook

    References

    Chapter Ten. Probing the Plasma Membrane Organization in Living Cells by Spot Variation Fluorescence Correlation Spectroscopy

    Abbreviations

    1 Introduction

    2 Optical Setups for Sizing the Excitation Volume

    3 General Considerations for svFCS Acquisition

    4 Measurements on Living Cells

    5 svFCS Data Analysis and Curve Fitting

    6 The Nature of the Molecular Constraints on Lateral Diffusion

    7 Summary and Future Outlook

    References

    Author Index

    Subject Index

Product details

  • No. of pages: 376
  • Language: English
  • Copyright: © Academic Press 2013
  • Published: December 29, 2012
  • Imprint: Academic Press
  • Hardcover ISBN: 9780124055391
  • eBook ISBN: 9780124058576

About the Serial Volume Editor

Sergey Tetin