Description

This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers Fluorescence Fluctuation Spectroscopy
Contains chapters on such topics as Time-integrated fluorescence cumulant analysis, Pulsed Interleaved Excitation, and raster image correlation spectroscopy and number and brightness analysis.

Key Features

  • Continues the legacy of this premier serial with quality chapters authored by leaders in the field
  • Covers fluorescence fluctuation spectroscopy
  • Contains chapters on such topics as time-integrated fluorescence cumulant analysis, pulsed interleaved excitation, and raster image correlation spectroscopy and number and brightness analysis.

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of Contents

Series page

Contributors

Preface

Methods in Enzymology

Chapter One. 40 Years of FCS: How It All Began

References

Chapter Two. Brief Introduction to Fluorescence Correlation Spectroscopy

1 Introduction

2 Conceptual Basis and Theory of FCS

3 Measurements Based on Fluctuation Amplitudes

4 Experimental Measurements

5 Close Relationship Between FCS and Fluorescence Photobleaching

6 Scanning and Imaging Approaches to FCS

7 A Brief and Partial Survey of Applications

8 Summary

References

Chapter Three. Dual-Color Fluorescence Cross-Correlation Spectroscopy with Continuous Laser Excitation in a Confocal Setup

1 Introduction

2 FCS in a Nutshell

3 The Dual-Color FCCS Extension

4 Data Analysis

5 Ligand Binding at the Cell Surface

6 Conclusion

References

Chapter Four. Brightness Analysis

1 Introduction

2 Background

3 Overview of Analysis Techniques

4 Brightness Analysis

5 Experimental Considerations

6 Brightness and Geometry

7 Summary

References

Chapter Five. Time-Integrated Fluorescence Cumulant Analysis and Its Application in Living Cells

1 Introduction

2 Theory and Implementation of TIFCA

3 Application of TIFCA

4 Conclusion

References

Chapter Six. Raster Image Correlation Spectroscopy and Number and Brightness Analysis

1 Introduction

2 A Conceptual Overview of Fluctuation Methods

3 What Is Different in the RICS Method?

4 RICS and Cross-RICS

5 PCH and Amplitude Fluctuation Analysis

6 N&B and Cross-NB

7 Simulations for Cross-RICS and Cross-N&B

8 Applications of RICS and N&B to Detect Molecular Complexes in Cells

9 Calibration Measurements using EGFP and mCherry and Effective Bleedthroug

Details

No. of pages:
336
Language:
English
Copyright:
© 2013
Published:
Imprint:
Academic Press
Print ISBN:
9780123884220
Electronic ISBN:
9780123884237