Fluorescence Fluctuation Spectroscopy (FFS), Part A

Fluorescence Fluctuation Spectroscopy (FFS), Part A

1st Edition - December 28, 2012
This is the Latest Edition
  • Editor: Sergey Tetin
  • Hardcover ISBN: 9780123884220
  • eBook ISBN: 9780123884237

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Description

This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers Fluorescence Fluctuation SpectroscopyContains chapters on such topics as Time-integrated fluorescence cumulant analysis, Pulsed Interleaved Excitation, and raster image correlation spectroscopy and number and brightness analysis.

Key Features

  • Continues the legacy of this premier serial with quality chapters authored by leaders in the field
  • Covers fluorescence fluctuation spectroscopy
  • Contains chapters on such topics as time-integrated fluorescence cumulant analysis, pulsed interleaved excitation, and raster image correlation spectroscopy and number and brightness analysis.

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of Contents

  • Series page

    Contributors

    Preface

    Methods in Enzymology

    Chapter One. 40 Years of FCS: How It All Began

    References

    Chapter Two. Brief Introduction to Fluorescence Correlation Spectroscopy

    1 Introduction

    2 Conceptual Basis and Theory of FCS

    3 Measurements Based on Fluctuation Amplitudes

    4 Experimental Measurements

    5 Close Relationship Between FCS and Fluorescence Photobleaching

    6 Scanning and Imaging Approaches to FCS

    7 A Brief and Partial Survey of Applications

    8 Summary

    References

    Chapter Three. Dual-Color Fluorescence Cross-Correlation Spectroscopy with Continuous Laser Excitation in a Confocal Setup

    1 Introduction

    2 FCS in a Nutshell

    3 The Dual-Color FCCS Extension

    4 Data Analysis

    5 Ligand Binding at the Cell Surface

    6 Conclusion

    References

    Chapter Four. Brightness Analysis

    1 Introduction

    2 Background

    3 Overview of Analysis Techniques

    4 Brightness Analysis

    5 Experimental Considerations

    6 Brightness and Geometry

    7 Summary

    References

    Chapter Five. Time-Integrated Fluorescence Cumulant Analysis and Its Application in Living Cells

    1 Introduction

    2 Theory and Implementation of TIFCA

    3 Application of TIFCA

    4 Conclusion

    References

    Chapter Six. Raster Image Correlation Spectroscopy and Number and Brightness Analysis

    1 Introduction

    2 A Conceptual Overview of Fluctuation Methods

    3 What Is Different in the RICS Method?

    4 RICS and Cross-RICS

    5 PCH and Amplitude Fluctuation Analysis

    6 N&B and Cross-NB

    7 Simulations for Cross-RICS and Cross-N&B

    8 Applications of RICS and N&B to Detect Molecular Complexes in Cells

    9 Calibration Measurements using EGFP and mCherry and Effective Bleedthrough

    10 Difference of Distribution in the Nucleus Versus Cytoplasm of Dynamin-2a

    11 Conclusions and Future Prospects

    12 Materials and Methods

    References

    Chapter Seven. Global Analysis in Fluorescence Correlation Spectroscopy and Fluorescence Lifetime Microscopy

    1 Introduction

    2 Background

    3 Theory

    4 Methods

    5 Results

    6 Conclusions

    References

    Chapter Eight. Dual-Focus Fluorescence Correlation Spectroscopy

    1 Introduction

    2 Dual-Focus Fluorescence Correlation Spectroscopy and Translational Diffusion

    3 Flow Measurements with 2fFCS

    4 2fFCS Measurements in Lipid Membranes

    5 Summary

    References

    Chapter Nine. Pulsed Interleaved Excitation

    1 Introduction: The Need for PIE

    2 How Does PIE Work?

    3 Juggling Photons with PIE

    4 Simple and Quantitative Correlation Analysis with PIE

    5 Quantifying FRET with PIE–FRET–FCCS

    6 Accurate Single-Pair FRET with MFD–PIE

    7 Scanning FCCS with PIE

    8 Glancing at the Future for Multicolor Spectroscopy

    References

    Chapter Ten. Image Correlation Spectroscopy: Mapping Correlations in Space, Time, and Reciprocal Space

    1 Introduction

    2 Theory of ICS

    3 Procedures for STICS and kICS

    4 Conclusions

    References

    Author Index

    Subject Index

Product details

  • No. of pages: 336
  • Language: English
  • Copyright: © Academic Press 2013
  • Published: December 28, 2012
  • Imprint: Academic Press
  • Hardcover ISBN: 9780123884220
  • eBook ISBN: 9780123884237

About the Serial Volume Editor

Sergey Tetin