Secure CheckoutPersonal information is secured with SSL technology.
Free ShippingFree global shipping
No minimum order.
Methods in Enzymology
Chapter One. 40 Years of FCS: How It All Began
Chapter Two. Brief Introduction to Fluorescence Correlation Spectroscopy
2 Conceptual Basis and Theory of FCS
3 Measurements Based on Fluctuation Amplitudes
4 Experimental Measurements
5 Close Relationship Between FCS and Fluorescence Photobleaching
6 Scanning and Imaging Approaches to FCS
7 A Brief and Partial Survey of Applications
Chapter Three. Dual-Color Fluorescence Cross-Correlation Spectroscopy with Continuous Laser Excitation in a Confocal Setup
2 FCS in a Nutshell
3 The Dual-Color FCCS Extension
4 Data Analysis
5 Ligand Binding at the Cell Surface
Chapter Four. Brightness Analysis
3 Overview of Analysis Techniques
4 Brightness Analysis
5 Experimental Considerations
6 Brightness and Geometry
Chapter Five. Time-Integrated Fluorescence Cumulant Analysis and Its Application in Living Cells
2 Theory and Implementation of TIFCA
3 Application of TIFCA
Chapter Six. Raster Image Correlation Spectroscopy and Number and Brightness Analysis
2 A Conceptual Overview of Fluctuation Methods
3 What Is Different in the RICS Method?
4 RICS and Cross-RICS
5 PCH and Amplitude Fluctuation Analysis
6 N&B and Cross-NB
7 Simulations for Cross-RICS and Cross-N&B
8 Applications of RICS and N&B to Detect Molecular Complexes in Cells
9 Calibration Measurements using EGFP and mCherry and Effective Bleedthrough
10 Difference of Distribution in the Nucleus Versus Cytoplasm of Dynamin-2a
11 Conclusions and Future Prospects
12 Materials and Methods
Chapter Seven. Global Analysis in Fluorescence Correlation Spectroscopy and Fluorescence Lifetime Microscopy
Chapter Eight. Dual-Focus Fluorescence Correlation Spectroscopy
2 Dual-Focus Fluorescence Correlation Spectroscopy and Translational Diffusion
3 Flow Measurements with 2fFCS
4 2fFCS Measurements in Lipid Membranes
Chapter Nine. Pulsed Interleaved Excitation
1 Introduction: The Need for PIE
2 How Does PIE Work?
3 Juggling Photons with PIE
4 Simple and Quantitative Correlation Analysis with PIE
5 Quantifying FRET with PIE–FRET–FCCS
6 Accurate Single-Pair FRET with MFD–PIE
7 Scanning FCCS with PIE
8 Glancing at the Future for Multicolor Spectroscopy
Chapter Ten. Image Correlation Spectroscopy: Mapping Correlations in Space, Time, and Reciprocal Space
2 Theory of ICS
3 Procedures for STICS and kICS
This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers Fluorescence Fluctuation Spectroscopy
Contains chapters on such topics as Time-integrated fluorescence cumulant analysis, Pulsed Interleaved Excitation, and raster image correlation spectroscopy and number and brightness analysis.
- Continues the legacy of this premier serial with quality chapters authored by leaders in the field
- Covers fluorescence fluctuation spectroscopy
- Contains chapters on such topics as time-integrated fluorescence cumulant analysis, pulsed interleaved excitation, and raster image correlation spectroscopy and number and brightness analysis.
Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists
- No. of pages:
- © Academic Press 2013
- 15th January 2013
- Academic Press
- Hardcover ISBN:
- eBook ISBN:
Elsevier.com visitor survey
We are always looking for ways to improve customer experience on Elsevier.com.
We would like to ask you for a moment of your time to fill in a short questionnaire, at the end of your visit.
If you decide to participate, a new browser tab will open so you can complete the survey after you have completed your visit to this website.
Thanks in advance for your time.