Description

One of the major challenges of modern biology and medicine consists in finding means to visualize biomolecules in their natural environment with the greatest level of accuracy, so as to gain insight into their properties and behaviour in a physiological and pathological setting. This has been achieved thanks to the design of novel imaging agents, in particular to fluorescent biosensors.

Fluorescence Biosensors comprise a large set of tools which are useful for fundamental purposes as well as for applications in biomedicine, drug discovery and biotechnology. These tools have been designed and engineered thanks to the combined efforts of chemists and biologists over the last decade, and developed hand in hand together with imaging technologies.

This volume will convey the many exciting developments the field of fluorescent biosensors and reporters has witnessed over the recent years, from concepts to applications, including chapters on the chemistry of fluorescent probes, on technologies for monitoring protein/protein interactions and technologies for imaging biosensors in cultured cells and in vivo. Other chapters are devoted to specific examples of genetically-encoded reporters, or to protein and peptide biosensors, together with examples illustrating their application to cellular and in vivo imaging, biomedical applications, drug discovery and high throughput screening.

Key Features

  • Contributions from leading authorities
  • Informs and updates on all the latest developments in the field

Readership

Researchers in imaging and fluorescence or biosensors and bioelectronics

Table of Contents

Contributors

Preface

Introduction

Chapter One. The Chemistry of Small-Molecule Fluorogenic Probes

1 Introduction

2 Fluorescence and Fluorophores

3 Modes of Fluorescence Modulation

4 Coumarins

5 Bodipy Dyes

6 Fluoresceins

7 Rhodamines

8 Phenoxazine Dyes

9 Acridinones

10 Cyanines

11 Other Fluorogenic Scaffolds

12 Conclusions and Future Directions

References

Chapter Two. Fluorescent Environment-Sensitive Dyes as Reporters of Biomolecular Interactions

1 Introduction

2 Single-Band Solvatochromic Dyes: Design and Applications

3 Two-Band Solvatochromic Dyes Based on Esipt

4 Applications of Two-Color Dyes for Monitoring Biomolecular Interactions

5 Conclusions

References

Chapter Three. Rational Design of Fluorophores for In Vivo Applications

1 Introduction

2 Cyanine and Related Fluorophores

3 Squaraines

4 Boron Dipyrromethene and Related Fluorophores

5 Porphyrins, Phthalocyanines, and Related Macrocycles

6 Special Types of Fluorophores

7 Conclusion

References

Chapter Four. Fluorescence Technologies for Monitoring Interactions Between Biological Molecules In Vitro

Abbreviations

1 Introduction to Fluorescence

2 Monitoring Protein/Substrate Interactions

3 Monitoring Protein/Protein and Polypeptide Interactions

4 Fluorescence for Protein/Membrane Interactions

5 Conclusions and Perspectives

References

Chapter Five. From FRET Imaging to Practical Methodology for Kinase Activity Sensing in Living Cells

Abbreviations

1 Introduction

2 Fluorescence Generalities

3 FRET Measurement

4 FRET Measurements: Methods and Instrumentation

5 Data Analysis

6 Design and Optimization of Genetically Encoded KARs

Details

No. of pages:
416
Language:
English
Copyright:
© 2013
Published:
Imprint:
Academic Press
Electronic ISBN:
9780123869517
Print ISBN:
9780123869326

About the serial-volume-editor

Reviews

Praise for the series:
"Full of interest not only for the molecular biologist-for whom the numerous references will be invaluable-but will also appeal to a much wider circle of biologists, and in fact to all those who are concerned with the living cell." --British Medical Journal