
Fluorescence-Based Biosensors
From Concepts to Applications
Description
Key Features
- Contributions from leading authorities
- Informs and updates on all the latest developments in the field
Readership
Researchers in imaging and fluorescence or biosensors and bioelectronics
Table of Contents
Contributors
Preface
Introduction
Chapter One. The Chemistry of Small-Molecule Fluorogenic Probes
1 Introduction
2 Fluorescence and Fluorophores
3 Modes of Fluorescence Modulation
4 Coumarins
5 Bodipy Dyes
6 Fluoresceins
7 Rhodamines
8 Phenoxazine Dyes
9 Acridinones
10 Cyanines
11 Other Fluorogenic Scaffolds
12 Conclusions and Future Directions
References
Chapter Two. Fluorescent Environment-Sensitive Dyes as Reporters of Biomolecular Interactions
1 Introduction
2 Single-Band Solvatochromic Dyes: Design and Applications
3 Two-Band Solvatochromic Dyes Based on Esipt
4 Applications of Two-Color Dyes for Monitoring Biomolecular Interactions
5 Conclusions
References
Chapter Three. Rational Design of Fluorophores for In Vivo Applications
1 Introduction
2 Cyanine and Related Fluorophores
3 Squaraines
4 Boron Dipyrromethene and Related Fluorophores
5 Porphyrins, Phthalocyanines, and Related Macrocycles
6 Special Types of Fluorophores
7 Conclusion
References
Chapter Four. Fluorescence Technologies for Monitoring Interactions Between Biological Molecules In Vitro
Abbreviations
1 Introduction to Fluorescence
2 Monitoring Protein/Substrate Interactions
3 Monitoring Protein/Protein and Polypeptide Interactions
4 Fluorescence for Protein/Membrane Interactions
5 Conclusions and Perspectives
References
Chapter Five. From FRET Imaging to Practical Methodology for Kinase Activity Sensing in Living Cells
Abbreviations
1 Introduction
2 Fluorescence Generalities
3 FRET Measurement
4 FRET Measurements: Methods and Instrumentation
5 Data Analysis
6 Design and Optimization of Genetically Encoded KARs
7 Considerations for KAR Measurements
8 Toward Quantitative Approaches in Biological Processes
9 Outlook and Perspective
References
Chapter Six. Fluorescent Sensors of Protein Kinases
Abbreviations
1 Introduction
2 Genetically Encoded Reporters of Protein Kinases
3 Fluorescent Peptide/Protein Biosensors
4 Applications
5 Conclusions and Perspectives
References
Chapter Seven. Time-Resolved Förster Resonance Energy Transfer-Based Technologies to Investigate G Protein-Coupled Receptor Machinery
1 Introduction
2 Overview of the TR-FRET Principle and Its Advantages
3 Screening with Generic Methods
4 Screening with Target-Specific Methods
5 Perspectives
6 Conclusion
References
Chapter Eight. Fluorescent Protein-Based Biosensors and Their Clinical Applications
1 Introduction
2 Fluorescent Proteins
3 Single-Fluorescent Protein-Based Biosensors
4 Bimolecular Fluorescence Complementation
5 Förster Resonance Energy Transfer
6 Methods for Evaluating FRET Efficiency
7 Clinical Application of FRET
8 Closing Remarks
References
Chapter Nine. Fluorescent Macromolecular Sensors of Enzymatic Activity for In Vivo Imaging
1 Introduction
2 Enzyme Activity and Dysregulation as a Predictor of Disease
3 Fluorescence Detection In Vivo
4 Principles of Enzymatic Activity Sensors Based on Fluorescence Changes
5 Macromolecular Fluorescent Probes
6 Pharmacokinetics of PGC and Imaging of Activation
7 Applications of Macromolecular Fluorescent Sensors in Cancer Imaging
8 Alternative Enzyme-Targeted Strategies in Imaging Cancer
9 Macromolecular Sensors in Fluorescent Imaging of Inflammation and Vascular Disease
10 Conclusions
References
Chapter Ten. Fluorescent Proteins as Visible In Vivo Sensors
1 Introduction
2 Noninvasive Imaging
3 Lighting Up the Tumor Stroma with Fluorescent Proteins
4 Stroma Cells are Required for Cancer Metastasis
5 Fluorescent Tumorgrafts made from Human Cancer Patients
6 Real-Time Imaging of Trafficking Cancer Cells
7 Method of Choice for Whole-Body Imaging
8 Conclusions
References
Index
Product details
- No. of pages: 416
- Language: English
- Copyright: © Academic Press 2013
- Published: December 31, 2012
- Imprint: Academic Press
- eBook ISBN: 9780123869517
- Hardcover ISBN: 9780123869326