Fluorescence-Based Biosensors

Fluorescence-Based Biosensors

From Concepts to Applications

1st Edition - December 31, 2012

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  • Editor: May C. Morris
  • eBook ISBN: 9780123869517
  • Hardcover ISBN: 9780123869326

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One of the major challenges of modern biology and medicine consists in finding means to visualize biomolecules in their natural environment with the greatest level of accuracy, so as to gain insight into their properties and behaviour in a physiological and pathological setting. This has been achieved thanks to the design of novel imaging agents, in particular to fluorescent biosensors. Fluorescence Biosensors comprise a large set of tools which are useful for fundamental purposes as well as for applications in biomedicine, drug discovery and biotechnology. These tools have been designed and engineered thanks to the combined efforts of chemists and biologists over the last decade, and developed hand in hand together with imaging technologies. This volume will convey the many exciting developments the field of fluorescent biosensors and reporters has witnessed over the recent years, from concepts to applications, including chapters on the chemistry of fluorescent probes, on technologies for monitoring protein/protein interactions and technologies for imaging biosensors in cultured cells and in vivo. Other chapters are devoted to specific examples of genetically-encoded reporters, or to protein and peptide biosensors, together with examples illustrating their application to cellular and in vivo imaging, biomedical applications, drug discovery and high throughput screening.

Key Features

  • Contributions from leading authorities
  • Informs and updates on all the latest developments in the field


Researchers in imaging and fluorescence or biosensors and bioelectronics

Table of Contents

  • Contributors



    Chapter One. The Chemistry of Small-Molecule Fluorogenic Probes

    1 Introduction

    2 Fluorescence and Fluorophores

    3 Modes of Fluorescence Modulation

    4 Coumarins

    5 Bodipy Dyes

    6 Fluoresceins

    7 Rhodamines

    8 Phenoxazine Dyes

    9 Acridinones

    10 Cyanines

    11 Other Fluorogenic Scaffolds

    12 Conclusions and Future Directions


    Chapter Two. Fluorescent Environment-Sensitive Dyes as Reporters of Biomolecular Interactions

    1 Introduction

    2 Single-Band Solvatochromic Dyes: Design and Applications

    3 Two-Band Solvatochromic Dyes Based on Esipt

    4 Applications of Two-Color Dyes for Monitoring Biomolecular Interactions

    5 Conclusions


    Chapter Three. Rational Design of Fluorophores for In Vivo Applications

    1 Introduction

    2 Cyanine and Related Fluorophores

    3 Squaraines

    4 Boron Dipyrromethene and Related Fluorophores

    5 Porphyrins, Phthalocyanines, and Related Macrocycles

    6 Special Types of Fluorophores

    7 Conclusion


    Chapter Four. Fluorescence Technologies for Monitoring Interactions Between Biological Molecules In Vitro


    1 Introduction to Fluorescence

    2 Monitoring Protein/Substrate Interactions

    3 Monitoring Protein/Protein and Polypeptide Interactions

    4 Fluorescence for Protein/Membrane Interactions

    5 Conclusions and Perspectives


    Chapter Five. From FRET Imaging to Practical Methodology for Kinase Activity Sensing in Living Cells


    1 Introduction

    2 Fluorescence Generalities

    3 FRET Measurement

    4 FRET Measurements: Methods and Instrumentation

    5 Data Analysis

    6 Design and Optimization of Genetically Encoded KARs

    7 Considerations for KAR Measurements

    8 Toward Quantitative Approaches in Biological Processes

    9 Outlook and Perspective


    Chapter Six. Fluorescent Sensors of Protein Kinases


    1 Introduction

    2 Genetically Encoded Reporters of Protein Kinases

    3 Fluorescent Peptide/Protein Biosensors

    4 Applications

    5 Conclusions and Perspectives


    Chapter Seven. Time-Resolved Förster Resonance Energy Transfer-Based Technologies to Investigate G Protein-Coupled Receptor Machinery

    1 Introduction

    2 Overview of the TR-FRET Principle and Its Advantages

    3 Screening with Generic Methods

    4 Screening with Target-Specific Methods

    5 Perspectives

    6 Conclusion


    Chapter Eight. Fluorescent Protein-Based Biosensors and Their Clinical Applications

    1 Introduction

    2 Fluorescent Proteins

    3 Single-Fluorescent Protein-Based Biosensors

    4 Bimolecular Fluorescence Complementation

    5 Förster Resonance Energy Transfer

    6 Methods for Evaluating FRET Efficiency

    7 Clinical Application of FRET

    8 Closing Remarks


    Chapter Nine. Fluorescent Macromolecular Sensors of Enzymatic Activity for In Vivo Imaging

    1 Introduction

    2 Enzyme Activity and Dysregulation as a Predictor of Disease

    3 Fluorescence Detection In Vivo

    4 Principles of Enzymatic Activity Sensors Based on Fluorescence Changes

    5 Macromolecular Fluorescent Probes

    6 Pharmacokinetics of PGC and Imaging of Activation

    7 Applications of Macromolecular Fluorescent Sensors in Cancer Imaging

    8 Alternative Enzyme-Targeted Strategies in Imaging Cancer

    9 Macromolecular Sensors in Fluorescent Imaging of Inflammation and Vascular Disease

    10 Conclusions


    Chapter Ten. Fluorescent Proteins as Visible In Vivo Sensors

    1 Introduction

    2 Noninvasive Imaging

    3 Lighting Up the Tumor Stroma with Fluorescent Proteins

    4 Stroma Cells are Required for Cancer Metastasis

    5 Fluorescent Tumorgrafts made from Human Cancer Patients

    6 Real-Time Imaging of Trafficking Cancer Cells

    7 Method of Choice for Whole-Body Imaging

    8 Conclusions



Product details

  • No. of pages: 416
  • Language: English
  • Copyright: © Academic Press 2013
  • Published: December 31, 2012
  • Imprint: Academic Press
  • eBook ISBN: 9780123869517
  • Hardcover ISBN: 9780123869326

About the Serial Volume Editor

May C. Morris

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