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Fluorescence and Phosphorescence Spectroscopy - 1st Edition - ISBN: 9780080204994, 9781483160993

Fluorescence and Phosphorescence Spectroscopy

1st Edition

Physicochemical Principles and Practice

Author: Stephen G Schulman
Editors: R. Belcher H. Freiser
eBook ISBN: 9781483160993
Imprint: Pergamon
Published Date: 1st January 1977
Page Count: 298
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Fluorescence and Phosphorescence Spectroscopy: Physicochemical Principles and Practice deals with the physicochemical principles and applications of fluorescence and phosphorescence spectroscopy in experimental biology and chemistry. Topics covered include the absorption of light by molecules; instrumentation for the measurement of fluorescence and phosphorescence; solvent and acidity effects on electronic spectra; and polarization of fluorescence and phosphorescence. Comprised of four chapters, this book begins with a discussion on photophysical processes in isolated molecules and molecules in solution, paying particular attention to thermal equilibration of electronically excited molecules, phototautomerism, and coordination by metal ions. The next chapter describes the instrumentation for measuring fluorescence and phosphorescence, which consists essentially of a light source to electronically excite the sample; a monochromator to separate the light of desired energy from the source; a sample compartment; a second monochromator to isolate the sample's fluorescence energy from the excitation energy; a photodetector to translate the fluorescent light into an electrical signal; and a readout system such as a galvanometer or a recorder, coupled with an amplifier to determine the intensity of fluorescent light that is emitted. The final chapter is devoted to various applications of fluorescence and phosphorescence spectroscopy, including the analysis of organic and inorganic compounds. This monograph is written primarily for analytical chemists and biological scientists.

Table of Contents

I. Photophysical Processes in Isolated Molecules

Molecular Electronic Structure

The Absorption of Light by Molecules

Vibrational Relaxation and Internal Conversion - Thermal Equilibration of Electronically Excited Molecules

Deactivation of the Thermally Equilibrated Lowest Excited Singlet State

II. Photophysical Processes in Molecules in Solution

Solvent Effects on Electronic Spectra

Acidity Effects on Electronic Spectra


Quenching of Molecular Luminescence

Coordination by Metal Ions

Concentration Effects

Fluorescence and Phosphorescence of Nucleic Acids

Polarization of Fluorescence and Phosphorescence

III. Instrumentation

Excitation Sources

Wavelength Selectors


Sample Compartments


Readout Systems

Instrumental Distortion of Spectra

Low Temperature Fluorescence

Phosphorescence Measurements

Sample Cells and Solvents for Phosphorimetry

Measurement of Fluorescence Quantum Yields

Measurement of Luminescence Lifetime

Measurement of Concentration

Time-Resolved Fluorometry

Selective Modulation Fluorometry


Table of Solvents for Phosphorimetry

IV. Applications

Organic Compounds

Inorganic Substances

Some Specialized Applications

Table of Fluorimetric Methods for Organic Compounds

Table of Phosphorimetric Methods for Organic Compounds

Table of Fluorimetric Methods for Inorganic Compounds



No. of pages:
© Pergamon 1977
1st January 1977
eBook ISBN:

About the Author

Stephen G Schulman

About the Editors

R. Belcher

H. Freiser

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