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Fluorescence and Phosphorescence Spectroscopy: Physicochemical Principles and Practice deals with the physicochemical principles and applications of fluorescence and phosphorescence spectroscopy in experimental biology and chemistry. Topics covered include the absorption of light by molecules; instrumentation for the measurement of fluorescence and phosphorescence; solvent and acidity effects on electronic spectra; and polarization of fluorescence and phosphorescence. Comprised of four chapters, this book begins with a discussion on photophysical processes in isolated molecules and molecules in solution, paying particular attention to thermal equilibration of electronically excited molecules, phototautomerism, and coordination by metal ions. The next chapter describes the instrumentation for measuring fluorescence and phosphorescence, which consists essentially of a light source to electronically excite the sample; a monochromator to separate the light of desired energy from the source; a sample compartment; a second monochromator to isolate the sample's fluorescence energy from the excitation energy; a photodetector to translate the fluorescent light into an electrical signal; and a readout system such as a galvanometer or a recorder, coupled with an amplifier to determine the intensity of fluorescent light that is emitted. The final chapter is devoted to various applications of fluorescence and phosphorescence spectroscopy, including the analysis of organic and inorganic compounds. This monograph is written primarily for analytical chemists and biological scientists.
I. Photophysical Processes in Isolated Molecules
Molecular Electronic Structure
The Absorption of Light by Molecules
Vibrational Relaxation and Internal Conversion - Thermal Equilibration of Electronically Excited Molecules
Deactivation of the Thermally Equilibrated Lowest Excited Singlet State
II. Photophysical Processes in Molecules in Solution
Solvent Effects on Electronic Spectra
Acidity Effects on Electronic Spectra
Quenching of Molecular Luminescence
Coordination by Metal Ions
Fluorescence and Phosphorescence of Nucleic Acids
Polarization of Fluorescence and Phosphorescence
Instrumental Distortion of Spectra
Low Temperature Fluorescence
Sample Cells and Solvents for Phosphorimetry
Measurement of Fluorescence Quantum Yields
Measurement of Luminescence Lifetime
Measurement of Concentration
Selective Modulation Fluorometry
Table of Solvents for Phosphorimetry
Some Specialized Applications
Table of Fluorimetric Methods for Organic Compounds
Table of Phosphorimetric Methods for Organic Compounds
Table of Fluorimetric Methods for Inorganic Compounds
- No. of pages:
- © Pergamon 1977
- 1st January 1977
- eBook ISBN:
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