Fluorescence and Phosphorescence Spectroscopy

Fluorescence and Phosphorescence Spectroscopy

Physicochemical Principles and Practice

1st Edition - January 1, 1977

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  • Author: Stephen G Schulman
  • eBook ISBN: 9781483160993

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Fluorescence and Phosphorescence Spectroscopy: Physicochemical Principles and Practice deals with the physicochemical principles and applications of fluorescence and phosphorescence spectroscopy in experimental biology and chemistry. Topics covered include the absorption of light by molecules; instrumentation for the measurement of fluorescence and phosphorescence; solvent and acidity effects on electronic spectra; and polarization of fluorescence and phosphorescence. Comprised of four chapters, this book begins with a discussion on photophysical processes in isolated molecules and molecules in solution, paying particular attention to thermal equilibration of electronically excited molecules, phototautomerism, and coordination by metal ions. The next chapter describes the instrumentation for measuring fluorescence and phosphorescence, which consists essentially of a light source to electronically excite the sample; a monochromator to separate the light of desired energy from the source; a sample compartment; a second monochromator to isolate the sample's fluorescence energy from the excitation energy; a photodetector to translate the fluorescent light into an electrical signal; and a readout system such as a galvanometer or a recorder, coupled with an amplifier to determine the intensity of fluorescent light that is emitted. The final chapter is devoted to various applications of fluorescence and phosphorescence spectroscopy, including the analysis of organic and inorganic compounds. This monograph is written primarily for analytical chemists and biological scientists.

Table of Contents

  • I. Photophysical Processes in Isolated Molecules

    Molecular Electronic Structure

    The Absorption of Light by Molecules

    Vibrational Relaxation and Internal Conversion - Thermal Equilibration of Electronically Excited Molecules

    Deactivation of the Thermally Equilibrated Lowest Excited Singlet State

    II. Photophysical Processes in Molecules in Solution

    Solvent Effects on Electronic Spectra

    Acidity Effects on Electronic Spectra


    Quenching of Molecular Luminescence

    Coordination by Metal Ions

    Concentration Effects

    Fluorescence and Phosphorescence of Nucleic Acids

    Polarization of Fluorescence and Phosphorescence

    III. Instrumentation

    Excitation Sources

    Wavelength Selectors


    Sample Compartments


    Readout Systems

    Instrumental Distortion of Spectra

    Low Temperature Fluorescence

    Phosphorescence Measurements

    Sample Cells and Solvents for Phosphorimetry

    Measurement of Fluorescence Quantum Yields

    Measurement of Luminescence Lifetime

    Measurement of Concentration

    Time-Resolved Fluorometry

    Selective Modulation Fluorometry


    Table of Solvents for Phosphorimetry

    IV. Applications

    Organic Compounds

    Inorganic Substances

    Some Specialized Applications

    Table of Fluorimetric Methods for Organic Compounds

    Table of Phosphorimetric Methods for Organic Compounds

    Table of Fluorimetric Methods for Inorganic Compounds


Product details

  • No. of pages: 298
  • Language: English
  • Copyright: © Pergamon 1977
  • Published: January 1, 1977
  • Imprint: Pergamon
  • eBook ISBN: 9781483160993

About the Author

Stephen G Schulman

About the Editors

R. Belcher

H. Freiser

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