Flow Cytometry, Part A

Flow Cytometry, Part A

2nd Edition - November 7, 1994
  • Editors: Zbigniew Darzynkiewicz, John Robinson, Harry Crissman
  • eBook ISBN: 9780080859392

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Description

Flow Cytometry, Second Edition provides a complete and comprehensive two volume laboratory guide and reference for the use of the most current methods in flow cytometry sample preparation and analysis. These essential techniques are described in a step-by-step format, supplemented by explanatory sections and trouble-shooting tips. The methods are accessible to all researchers and students in biomedical science and biology who must use flow cytometry to separate and analyze cells.

Key Features

@introbul:Key Features
@bul:* Completely revised and greatly expanded since the publication of the First Edition in 1990
* Methods cover cell death and cell cycle analyses
Practical, handbook-style presentation works in lab or classroom
* Unique comprehensive methodological coverage
* Color plates illustrate techniques
* In-depth treatment of procedures, including a description of each procedure:
@subbul:* Theoretical foundations
* Critical aspects
* Possible pitfalls
* Written by authors with extensive experience who:
@subbul:* Developed or modified the techniques
* Describe their experience with different instruments and applications to different cell systems
* Are the Who's Who in Flow Cytometry

Readership

Researchers, graduate students, and technicians in cell and developmental biology, pharmacology, toxicology, biochemistry, genetics and molecular biology, biotechnology, immunology, oncology, pathology, plant and marine science, and ecology.

Table of Contents

  • D.W. Visscher and J.D. Crissman, Dissociation of Intact Cells from Tumors and Normal Tissues.
    Z. Darzynkiewicz, X. Li, and J. Gong, Assays of Cell Viability: Discrimination of Cells Dying by Apoptosis.
    C.C. Stewart and S.J Stewart, Cell Preparation for the Identification of Leukocytes.
    C.C. Stewart and S.J. Stewart, Multiparameter Analysis of Leukocytes by Flow Cytometry.
    G.F. Babcock and S.M. Dawes, Immunophenotyping Using Fixed Cells.
    P.P.T.Brons, P.E.J. Van Erp, and A.H.M. Pennings, Simultaneous DNA Content and Cell-Surface Immunofluorescence Analysis.
    H.M. Shapiro, Cell Membrane Potential Analysis.
    M.J. Boyer and D.W. Hedley, Measurement of Intracellular pH.
    C.H. June and P.S. Rabinovitch, Intracellular Ionized Calcium.
    H.A. Crissman and J.A. Steinkamp, Cellular Protein Content Measurements.
    F. Traganos and Z. Darzynkiewicz, Lysosomal Proton Pump Activity: Supravital Cell Staining with Acridine Orange Differentiates Leukocyte Subpopulations.
    H.A. Crissman and G.T. Hirons, Staining of DNA in Live and Fixed Cells.
    F.J. Otto, High-Resolution Analysis of Nuclear DNA Employing the Fluorochrome DAPI.
    L.L. Vindeløv and I.J. Christensen, Detergent and Proteolytic Enzyme-Based Techniques for Nuclear Isolation and DNA Content Analysis.
    D.W. Hedley, DNA Analysis from Paraffin-Embedded Blocks.
    L.G. Dressler and L.C. Seamer, Controls, Standards, and Histogram Interpretation in DNA Flow Cytometry.
    P.S. Rabinovitch, DNA Content Histogram and Cell-Cycle Analysis.
    F. Dolbeare and J.R. Selden, Immunochemical Quantitation of Bromodeoxyuridine: Application to Cell-Cycle Kinetics.
    J.A. Aten, J. Stap, R. Hoebe, and P.J.M. Bakker, Application and Detection of IdUrd and CldUrd as Two Independent Cell-Cycle Markers.
    M. Poot, H. Hoehn, M. Kubbies, A. Grossmann, Y. Chen, and P.S. Rabinovitch, Cell-Cycle Analysis Using Continuous Bromodeoxyuridine Labeling and Hoechst 33358--Ethidium Bromide Bivariate Flow Cytometry.
    H.A. Crissman, N. Oishi, and R. Habbersett, Detection of BrdUrd-Labeled Cells by Differential Fluorescence Analysis of DNA Fluorochromes: Pulse-Chase and Continuous Labeling Methods.
    K.D. Bauer and J.W. Jacobberger, Analysis of Intracellular Proteins.
    J.K. Larsen, "Washless" Procedures for Nuclear Antigen Detection.
    W. Giaretti and M. Nüsse, Light Scatter of Isolated Cell Nuclei as a Parameter Discriminating the Cell-Cycle Subcompartments.
    Z. Darzynkiewicz, Simultaneous Analysis of Cellular RNA and DNA Content.
    Z. Darzynkiewicz, J. Gong, and F. Traganos, Analysis of DNA Content and Cyclin Protein Expression in Studies of DNA Ploidy, Growth Fraction, Lymphocyte Stimulation, and the Cell-Cycle.
    J.P. Robinson, W.O. Carter, and P.K. Narayanan, Oxidative Product Formation Analysis by Flow Cytometry.
    S. Klingel, G. Rothe, W. Kellermann, and G. Valet, Flow Cytometric Determination of Cysteine and Serine Proteinase Activities in Living Cells with Rhodamine 110 Substrates.
    J.J. Turek and J.P. Robinson, Leucine Aminopeptidase Activity by Flow Cytometry.
    J.V. Watson and C. Dive, Enzyme Kinetics.
    K. Nooter, H. Herweijer, R.R. Jonker, and G.J. van den Engh, On-Line Flow Cytometry: A Versatile Method for Kinetic Measurements.
    Z. Darzynkiewicz, Acid-Induced Denaturation of DNA in Situ as a Probe of Chromatin Structure.
    J. Laffin and J.M. Lehman, Detection of Intracellular Virus and Viral Products.
    Index.
    Volumes in Series.

Product details

  • No. of pages: 591
  • Language: English
  • Copyright: © Academic Press 1994
  • Published: November 7, 1994
  • Imprint: Academic Press
  • eBook ISBN: 9780080859392

About the Serial Volume Editors

Zbigniew Darzynkiewicz

Affiliations and Expertise

CANCER RESEARCH INSTITUTE OF THE NEW YORK MEDICAL COLLEGE AT VALHALLA

John Robinson

John P. Robinson is a Professor of Sociology at the University of Maryland, College Park. Dr. Robinson founded and directed the Survey Research Center at the University of Maryland and the Communication Research Center at Cleveland State University. He has published more than 100 articles in professional journals and books and is a contributing editor to American Demographics magazine. Dr. Robinson was an American Statistical Association/National Science Foundation fellow at the Bureau of Labor Statistics in 1992-93, a Fulbright scholar at Moscow State University and Soviet Academy of Sciences in 1990, a Research Consultant at BBC News in 1978 and Research Coordinator for the U.S. Surgeon General’s Program on Television and Human Behavior in 1970. He received the 1987 Fordham University McGannon Award for Social and Ethical Relevance in Communication Policy Research for his research on improving public understanding of the news. His areas of specialization include social science methodology, attitude and behavior measurement, social change, and the impact of mass communication and other home technology. Additionally, he is an Editor of the Encyclopedia of Food Science, Food Technology & Nutrition.

Affiliations and Expertise

Department of Sociology, University of Maryland, College Park, U.S.A.

Harry Crissman

Affiliations and Expertise

Cell and Molecular Biology Group, Los Alamos National Laboratory, New Mexico, U.S.A.

About the Serial Editors

Leslie Wilson

University of California, Santa Barbara, USA

Affiliations and Expertise

University of California, Santa Barbara, USA

Paul Matsudaira

Affiliations and Expertise

Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology, Cambridge, U.S.A.