1st Edition - May 23, 2006

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  • Editors: Fred Rainey, Aharon Oren
  • Hardcover ISBN: 9780125215367

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This latest volume in the Methods in Microbiology series provides an overview of the methods used for the isolation, cultivation and handling of a wide variety of extremophiles, both at the stage of their isolation from natural samples and in pure culture. It contains protocols specific for work with extremophiles, as well as adaptations of “standard” microbiology protocols modified to enable the handling of extremophiles. Emphasis will be on detailed time-tested protocols that are specific for work with extremophiles and adaptations of “standard” microbiology protocols that have to be modified to be suitable for handling extremophiles.

Key Features

* Guides newcomers in the field of extremophile microbiology in the techniques used
* Provides an overview of all areas of extremophile research
* Many protocols could be adapted for use with a wide range of organisms


General microbiologists, industrial microbiologists, biotechnologists, molecular biologists, microbial ecologists, envirnomental and marine microbiologists

Table of Contents

    In-situ activity studies in thermal environments
    The isolation of thermophiles from deep-sea hydrothermal environments
    Growth of hyperthermophilic microorganisms for physiological and nutritional studies
    Toward the large scale cultivation of hyperthermophiles at high temperature and high-pressure
    The chemotaxonomic analysis of lipids from extremophilic bacteria
    Membranes of thermophiles and other extremophiles
    Characterization and quantification of compatible solutes in (hyper)thermophilic microorganisms
    Functional genomics of the thermo-acidophilic archaeon Sulfolobus solfataricus
    Heat shock proteins in hyperthermophiles
    Deep-sea Thermococcales and their genetic elements: plasmids and viruses
    Genetic systems for Thermus
    Gene transfer systems for obligately anaerobic thermophilic bacteria
    Hyperthermophilic virus-host systems: detection and isolation
    Preservation of thermophilic microorganisms
    Handling psychrophilic microorganisms
    Proteins from psychrophiles
    Cultivation of aerobic alkaliphiles
    Isolation, cultivation, and characterization of alkalithermophiles
    The isolation and study of acidophilic microorganisms
    Characterization of natural communities of halophilic microorganisms
    Cultivation of haloarchaea
    Halophile extraction from ancient crystals
    The assessment of the viability of halophilic microorganisms in natural communities
    Characterization of lipids of halophilic Archaea
    Characterization of organic compatible solutes of halotolerant and halophilic organisms
    Genetic systems for halophilic archaea
    The isolation ?of viruses of halophilic microorganisms
    Detection, quantification and purification of halocins: peptide antibiotics from haloarchaeal extremophiles
    The storage of halophilic bacteria
    Handling piezophilic microorganisms
    Measuring survival in microbial populations following exposure to ionizing radiation
    Strictly anaerobic microorganisms
    Industrial screening of extemophiles for valuable biomolecules

Product details

  • No. of pages: 838
  • Language: English
  • Copyright: © Academic Press 2006
  • Published: May 23, 2006
  • Imprint: Academic Press
  • Hardcover ISBN: 9780125215367

About the Series Volume Editors

Fred Rainey

Affiliations and Expertise

College of Basic Sciences, Louisiana State University, Baton Rouge, U.S.A.

Aharon Oren

Affiliations and Expertise

Department of Plant and Environmental Sciences, The Institute of Life Sciences, The Hebrew University of Jerusalem, Israel

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