Extracellular Vesicles

Extracellular Vesicles

1st Edition - October 24, 2020
This is the Latest Edition
  • Editors: Sheila Spada, Lorenzo Galluzzi
  • eBook ISBN: 9780128206638
  • Hardcover ISBN: 9780128206621

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Extracellular Vesicles, Volume 645 in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Genetic labeling of extracellular vesicle exosomes for studying biogenesis and uptake in living mammalian cells, Fluorescent Labeling of Extracellular Vesicles, Isolation of extracellular vesicles from lymph, Transgenic rats for tracking body fluid/tissue-derived extracellular vesicles, Isolation of amniotic extracellular vesicles, Urinary extracellular vesicle isolation, Immunocapture-based ELISA to Characterize and Quantify Extracellular Vesicles in Both Cell Culture Supernatants and Body Fluids, and much more.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology series


Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of Contents

  • 1. Genetic labeling of extracellular exosomes for studying biogenesis and uptake in living mammalian cells

    Daniel Levy, Mai Anh Do, Annie Brown, Kyle Asano, David Diebold, Hanzhe Chen, Jiayi Zhang, Brendan Lawler and Biao Lu

    2. Fluorescent labeling of extracellular vesicles

    Mehdi Dehghani and Thomas R. Gaborski

    3. Use of antibody arrays to probe exosome and extracellular vesicle mediated functional changes in cells

    Grazia Ambrosini, Blake Ebert, Richard D. Carvajal, Gary K. Schwartz and Alex J. Rai

    4. Analysis of individual extracellular vesicles by imaging flow cytometry

    Tobias Tertel, André Görgens and Bernd Giebel

    5. Imaging intercellular interaction and extracellular vesicle exchange in a co-culture model of chronic lymphocytic leukemia and stromal cells by lattice light sheet fluorescence microscopy

    Sara Elgamal, Federico Colombo, Francesca Cottini, John C. Byrd and Emanuele Cocucci

    6. Methods to purify DNA from extracellular vesicles: Focus on exosomes

    Sheila Spada

    7. Quantification of mtDNA in extracellular vesicles

    Beata Soltes and Balint Nagy

    8. Deciphering the total RNA content of extracellular vesicles

    Maria G. Amorim, Emmanuel Dias-Neto and Diana N. Nunes

    9. Immunocapture-based ELISA to characterize and quantify exosomes in both cell culture supernatants and body fluids

    Mariantonia Logozzi, Rossella Di Raimo, Davide Mizzoni and Stefano Fais

    10. Isolation and characterization of human amniotic fluid-derived exosomes

    Samantha Sheller-Miller and Ramkumar Menon

    11. Human urine exosomes: Another important member of the liquid biopsy family

    Antonio De Maio

    12. Three-dimensional cell models for EV production, isolation, and characterization

    Liliia Paniushkina, Elena Grueso Navarro, Xu Chen and Irina Nazarenko

    13. Transgenic rats for tracking body fluid/tissue-derived extracellular vesicles

    Aya Yoshimura, Yoshitaka Tamai and Takahiro Ochiya

    14. Live tracking of extracellular vesicles in larval zebrafish

    Benjamin Mary, Shima Ghoroghi, Vincent Hyenne and Jacky G. Goetz

    15. Assessment of innate immune response activation following the injection of extracellular vesicles isolated from human cell cultures in zebrafish embryos

    Marco Cafora, Mirjam Hoxha, Laura Cantone, Valentina Bollati, Anna Pistocchi and Luca Ferrari

Product details

  • No. of pages: 314
  • Language: English
  • Copyright: © Academic Press 2020
  • Published: October 24, 2020
  • Imprint: Academic Press
  • eBook ISBN: 9780128206638
  • Hardcover ISBN: 9780128206621

About the Serial Volume Editors

Sheila Spada

Sheila Spada is at Weill Cornell Medical College, NY, USA

Affiliations and Expertise

Weill Cornell Medical College, NY, USA

Lorenzo Galluzzi

Lorenzo Galluzzi
Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology. Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals: OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience.

Affiliations and Expertise

Assistant Professor of Cell Biology in Radiation Oncology, Department of Radiation Oncology, Weill Cornell Medical College, NY, USA