Experimental Models of Infection, Inflammation and Injury

Experimental Models of Infection, Inflammation and Injury

1st Edition - March 31, 2022

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  • Editor: David Montrose
  • eBook ISBN: 9780323899468
  • Hardcover ISBN: 9780323899451

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Description

Experimental Models of Infection, Inflammation and Injury, Volume 168 provides step-by-step protocols for scientific researchers to effectively utilize experimental model systems. Chapters in this new release include Induction and Evaluation of Murine T Cell Transfer Colitis, Modelling acute graft-versus-host disease (aGVHD) in murine bone marrow transplantation (BMT) models with MHC disparity, Mouse models of Graft-Versus-Host Disease, Preclinical Model of Multiple Sclerosis: Methods in Autoimmune Demyelination, Preclinical model of Multiple Sclerosis: focal, chemical or demyelination, Investigating demyelination, efficient remyelination and remyelination failure in organotypic cerebellar slice cultures: workflow and practical tips, and more. Other notable sections cover Rheumatoid Arthritis: Methods for Two Murine Models, Induction of Pancreatitis in Mice with Susceptibility to Pancreatic Cancer, Small Animal Models of Thermal Injury, Large Animal Models of Thermal Injury, Small animal models of localized heart irradiation, Methods for Induction and Assessment of Intestinal Permeability in Rodent Models of Radiation Injury, and more.

Key Features

  • Provides precise, step-by-step guidance on how to implement experimental systems
  • Presents a comprehensive background on the disease the model is being used to study
  • Offers insights into how the described disease models compare to other existing systems

Readership

Scientific researchers who would like to utilize experimental systems to study infection, inflammation and injury

Table of Contents

  • Cover image
  • Title page
  • Table of Contents
  • Copyright
  • Contributors
  • Chapter 1: Induction and evaluation of murine colitis induced by T cell transfer
  • Abstract
  • 1: Introduction
  • 2: Reagents and equipment
  • 3: Harvesting and isolating splenocytes
  • 4: Enrichment of CD4+ T cells
  • 5: Antibody staining of naïve T cells
  • 6: Fluorescence-assisted cell sorting (FACS) of naïve T cells
  • 7: Injecting Rag1−/− mice with CD4+ CD45RBhigh T cells
  • 8: Monitoring clinical disease progression
  • 9: Tissue collection at the terminal time point
  • 10: Disease assessment using harvested tissue
  • 11: Concluding remarks
  • 12: Notes
  • Acknowledgments
  • References
  • Chapter 2: Modeling acute graft-versus-host disease (aGVHD) in murine bone marrow transplantation (BMT) models with MHC disparity
  • Abstract
  • 1: Introduction
  • 2: Conditioning of recipient mice
  • 3: Preparation of donor bone marrow cells
  • 4: Preparation of splenocytes
  • 5: Allogeneic BMT and clinical and histological GVHD assessment
  • 6: Analysis of transplant engraftment and chimerism
  • 7: The B6 → B6.bm1 BMT model
  • 8: The B6 → B6D2F1 BMT model
  • 9: Concluding remarks
  • 10: Notes
  • References
  • Chapter 3: Mouse models of graft-versus-host disease
  • Abstract
  • 1: Introduction
  • 2: GVHD protocols
  • 3: Notes
  • 4: Summary and future directions
  • Acknowledgments
  • Appendix A
  • References
  • Chapter 4: Preclinical model of multiple sclerosis: Methods in autoimmune demyelination
  • Abstract
  • 1: Introduction
  • 2: Active induction of EAE
  • 3: Passive induction of EAE
  • 4: Scoring of EAE disease severity
  • 5: Quantification of demyelination
  • 6: Concluding remarks
  • References
  • Chapter 5: Preclinical model of multiple sclerosis: Focal, chemical or viral demyelination
  • Abstract
  • 1: Introduction
  • 2: Cuprizone
  • 3: Lysolecithin
  • 4: Ethidium Bromide
  • 5: Viral models of demyelination Theiler's murine encephalitis virus (TMEV)
  • 6: Scoring for remyelination
  • 7: Concluding remarks
  • References
  • Chapter 6: Investigating demyelination, efficient remyelination and remyelination failure in organotypic cerebellar slice cultures: Workflow and practical tips
  • Abstract
  • 1: Introduction
  • 2: Materials
  • 3: Preparation
  • 4: Dissection
  • 5: Demyelination-remyelination experiments
  • 6: Concluding remarks
  • Acknowledgments
  • References
  • Chapter 7: Rheumatoid arthritis: Methods for two murine models
  • Abstract
  • 1: Introduction
  • 2: Collagen induced arthritis
  • 3: TNF induced arthritis
  • 4: General considerations
  • 5: Concluding remarks
  • 6: Notes
  • Funding
  • References
  • Chapter 8: Induction of pancreatitis in mice with susceptibility to pancreatic cancer
  • Abstract
  • 1: Introduction
  • 2: Materials
  • 3: Methods
  • 4: Notes
  • Acknowledgment
  • References
  • Chapter 9: Small animal models of thermal injury
  • Abstract
  • 1: Introduction
  • 2: Small animal models in burn studies
  • 3: Small animal models replicating clinical burn pathophysiology
  • 4: Post-burn responses in aged vs young animals
  • 5: Differential effects based on biological sex
  • 6: Scald burn model in rodents
  • 7: Notes
  • 8: Take-home messages
  • Acknowledgments
  • References
  • Chapter 10: Large animal models of thermal injury
  • Abstract
  • 1: Introduction
  • 2: Large animal models in burn research
  • 3: Large animal models replicating clinical burn pathophysiology
  • 4: Conclusion and perspective on animal models
  • 5: Contact burn model in Yorkshire pigs
  • Acknowledgments
  • References
  • Chapter 11: Small animal models of localized heart irradiation
  • Abstract
  • 1: Introduction
  • 2: A model of image-guided whole heart irradiation in small experimental animals
  • 3: Common histological assessments of cardiac radiation injury
  • 4: Concluding remarks
  • 5: Notes
  • Acknowledgments
  • References
  • Chapter 12: Methods for induction and assessment of intestinal permeability in rodent models of radiation injury
  • Abstract
  • 1: Introduction
  • 2: Models of radiation-induced gastrointestinal toxicity
  • 3: Evaluation of intestinal permeability
  • 4: Notes
  • Acknowledgments
  • References
  • Chapter 13: Methods and protocols for translatable rodent models of postsurgical pain
  • Abstract
  • 1: Introduction
  • 2: Procedures
  • 3: Behavioral outcomes and methods
  • 4: Concluding remarks
  • 5: Notes
  • References
  • Chapter 14: Methods and protocols for chemotherapy-induced peripheral neuropathy (CIPN) mouse models using paclitaxel
  • Abstract
  • 1: Introduction
  • 2: CIPN models
  • 3: Behavioral outcomes and methods
  • 4: Concluding remarks
  • 5: Notes
  • References
  • Chapter 15: The use of foodborne infection to evaluate bacterial pathogenesis and host response
  • Abstract
  • 1: Introduction
  • 2: Foodborne L. monocytogenes infection
  • 3: Enumerating bacterial burden
  • 4: Assessing the host response by flow cytometry
  • 5: Concluding remarks
  • 6: Notes
  • Acknowledgments
  • References
  • Chapter 16: Induction and analysis of systemic C. albicans infections in mice
  • Abstract
  • 1: Introduction
  • 2: Materials
  • 3: Methods
  • 4: Notes
  • References
  • Chapter 17: Methods to evaluate virus-mediated acute lung inflammation
  • Abstract
  • 1: Introduction
  • 2: Materials
  • 3: Methods
  • 4: Notes
  • References
  • Further reading

Product details

  • No. of pages: 358
  • Language: English
  • Copyright: © Academic Press 2022
  • Published: March 31, 2022
  • Imprint: Academic Press
  • eBook ISBN: 9780323899468
  • Hardcover ISBN: 9780323899451

About the Serial Volume Editor

David Montrose

Dr. Montrose received his B.A. in Biology from Manhattanville College and his Ph.D. in Biomedical Science from the University of Connecticut (Health Center campus). He received his post-doctoral training at Weill Cornell Medical College of Cornell University. He is currently an Assistant Professor and lab head in the Department of Pathology at Stony Brook University School of Medicine and a member of the Stony Brook Cancer Center. Dr. Montrose is also a member of the Molecular and Cellular Pharmacology (MCP) and Molecular Genetics and Microbiology (MGM) graduate programs.

Affiliations and Expertise

Assistant Professor and Lab Head, Department of Pathology, Stony Brook University School of Medicine, NY, USA

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