Experimental Biology with Micro-Organisms - 1st Edition - ISBN: 9780408702270, 9781483162928

Experimental Biology with Micro-Organisms

1st Edition

Students' Manual

Authors: J. W. Garbutt A. J. Bartlett
eBook ISBN: 9781483162928
Imprint: Butterworth-Heinemann
Published Date: 1st January 1972
Page Count: 186
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Experimental Biology with Micro-organisms: Students' Manual talks about micro-organisms and examines facts and different relevant studies. The first part of the book discusses handling, culturing, and observing a micro-organism; this part also explains the importance of such practices when dealing with the said subject. Also mentioned in this part are the nutrition of the micro-organisms and the explanations regarding autotrophs and heterotrophs and what complex food they manufacture or utilize. The book also presents a background on the life cycle of the organisms, such as bacteria, chlorella, slime molds, yeast, Mucor hiemalis, and Basidiomycetes. In Chapters 4 and 5, the book talks more about an organism's growth and genetics, along with some of its subtopics. The succeeding chapters focus more on the environment's effect on organisms. The book ends with an analysis of the different interactions. The book caters for people who are studying biology and acts as a great reference for bio research.

Table of Contents

Introduction 1 Handling, Culturing and Observing Micro-Organisms Expt. 1.1 Micro-Organisms—Their Variety and Their Habitats Expt. 1.2 Isolating a Pure Culture of Bacteria from a Mixed Population Expt. 1.3 Preparing Stained Preparations of Bacteria Appendix—Microscopy Expt. 1.4 Methods of Sterilization Appendix—Autoclaving 2 Nutrition Expt. 2.1 Nutrient Requirements in an Autotrophic Organism—Chlorella Expt. 2.2 Nutrient Requirements in a Heterotrophic Organism—Aspergillus niger Expt. 2.3 Protein Digestion in Bacteria Expt. 2.4 Starch Digestion in Aspergillus niger Expt. 2.5 The Effect of the Vitamin Thiamine on the Growth of Heterotrophic Organisms 3 Structure and Life Cycles Expt. 3.1 The Structure of Bacteria Appendix—Measurement Expt. 3.2 The Life Cycle of a Green Alga—Chlorella Expt. 3.3 The Life Cycle of a Slime Mould—Dictyostelium discoideum Expt. 3.4 The Life Cycle of a Non-Mycelial Ascomycete-Yeast Expt. 3.5 The Life Cycle of Mucor hiemalis Expt. 3.6 An Examination of Two Mycelial Ascomycetes-Aspergillus (Eurotium) and Penicillium Expt. 3.7 The Life Cycle of a Basidiomycete—Agaricus bisporus 4 Growth Expt. 4.1 The Growth of a Population of a Unicellular Organism—Chlorella Appendix—Hemocytometer Expt. 4.2 Growth of a Filamentous Organism—Mucor hiemalis 5 Genetics Expt. 5.1 To Demonstrate Gene Segregation and Crossing over Using an Ascomycete Expt. 5.2 Selecting Bacteria Mutants by Using Antibiotics Expt. 5.3 An Investigation into Biochemical Mutants in the Bread Mold—Neurospora crassa 6 Soil Ecology Expt. 6.1 Estimating the Numbers of Micro-Organisms in Soils Expt. 6.2 Cellulose Decomposition by Soil Micro-Organisms Expt. 6.3 Ammonification in Soils Expt. 6.4 Nitrification by Soil Organisms Expt. 6.5 Culturing Anaerobic Organisms from the Soil Expt. 6


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© Butterworth-Heinemann 1972
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About the Author

J. W. Garbutt

A. J. Bartlett

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