Experiences in Biochemical Perception

Experiences in Biochemical Perception

1st Edition - January 1, 1982

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  • Editor: L Ornston
  • eBook ISBN: 9780323152037

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Experiences in Biochemical Perception discusses the breadth of contributions of I. C. Gunsalus, a versatile biochemist, in the field of biochemistry. This book explores the mechanism of electron transport to protein structure and documents the interface between the Gunsalus laboratory and the physicists. Organized into five parts encompassing 27 chapters, this book starts with a description of metabolic processes and the study of pyruvate oxidation. This text then illustrates how studies of microbial respiration led to the discovery of pyridoxl phosphate. Other chapters review dehydrogenases that participate in the dissimilation of branch-chain aromatic acids. This book further discusses the phosphorus–nitrogen bond in phosphorylated proteins and the inactivation of glutamine synthetase. The final chapter describes the elucidation of the function of the coenzyme biotin and generalizes about the use of bacteria in nutritional studies. This book is a valuable resource for readers and researchers engaged in the study of genetics, enzymology, microbiology, chemistry, and spectroscopy.

Table of Contents

  • Contributors


    I Metabolism

    1 The Search for the Mechanism of Pyruvate Oxidation in 1951

    Enzymatic Synthesis of Citrate

    Pyruvate Oxidation


    2 A Decade of Pyruvate Systems



    3 Early Studies on Respiration and the Coenzyme Form of Vitamin B6



    4 Branched-Chain Ketoacid Dehydrogenase of Pseudomonas putida PpG2



    Requirement of Branched-Chain Ketoacid Dehydrogenase for Lipoamide Dehydrogenase from P. putida Grown on Valine

    Effect of L-Valine on Kinetics of Branched-Chain Ketoacid Dehydrogenase



    5 A Movable Feast with Gunny: An Early History of the Mandelate Pathway



    6 Our Microbial World



    7 Bacteria and the Challenge of Cyclic Molecules

    I. Introduction

    II. Effects of Camphor Vapor

    III. Metabolism of Cyclohexanol and Cyclopentanol

    IV. Metabolism of Alicyclic Acids

    V. Camphor Revisited


    8 Microbial Metabolism of Anthracycline Antibiotics

    I. Introduction

    II. Microbial Metabolism of Anthracycline Antibiotics

    III. Conclusion

    IV. Personal Note


    9 Fatty Acid and Electron Transport in Pseudomonads

    The First Generation

    The Second Generation


    II Genetics

    10 Order and Disorder in Genome Arrangement


    Mapping the Bacterial Genome

    The Genetics of Bacteria Other than E. coli K12

    The Genetics of P. putida

    The Promiscuous Plasmid

    Gene Arrangement and Regulatory Patterns

    The Plasmid—Nature's Wild Card


    11 Plasmids and Nutritional Diversity

    Regulation of Plasmid-Coded Enzymes

    Evolution of Degradative Plasmids


    12 Communication among Coevolving Genes

    Rearrangements among Structural and Regulatory Genes

    Extensive Divergence of Homologous Genes as They Coevolve within a Cell Line

    Similar Amino Acid Sequences in Enzymes with Different Catalytic Activities


    13 Mixed Regulation in a Biosynthetic Pathway: Learning How to Listen

    I. Discovery

    II. Attempt to Characterize Tryptophan Synthase Regulation in P. putida by Genetic Analysis

    III. Cloning Certain P. aeruginosa trp Genes in E. coli

    IV. Unsolved Problems and Future Experiments

    V. Envoi


    III Regulation

    14 Regulation of L-Threonine Dehydrase by Ligand-Induced Oligomerization: An Odyssey Born of the Pyridoxal Phosphate Era




    15 Organization and Expression of an E. coli Cluster of Genes Involved in the Translation Process

    Organization and Expression of the Initiation Factor IF3 E. coli Gene Cluster

    Translational Initiation Pathways

    Isolation of a Thermosensitive Strain Containing a Thermolabile IF3

    Order of the Genes in λp2 as Studied by Their Segregation Pattern in Deleted Phages

    Physical Location of the Genes on λp2

    Transcription of the Genes around infC

    The Expression of the Genes around infC Is Differently Regulated



    16 Posttranscriptional Control of Protein Synthesis during Substrate Adaptation



    17 From Bacterial Camphor Metabolism to Mammalian Protein Kinase Systems Regulated by Cyclic AMP, Cyclic GMP, and Calcium

    I. Introduction

    II. A Bacterial Dehydrogenase in Camphor Oxidation

    III. Mammalian Protein Kinase Systems

    IV. Concluding Remarks


    18 Phosphorus-Nitrogen Bonds in Proteins



    19 Regulation of Glutamine Synthetase Degradation

    I. Introduction

    II. Results

    III. Discussion


    IV Cytochrome P450 and Electron Transport

    20 From Stars to Organic Chemistry via Cytochrome P450


    The Illinois Biochemistry/Biophysics Group, 1972-1977


    21 Purification and Characterization of Adrenal P450 Cytochromes

    I. Introduction

    II. Purification of the Two P450 Proteins

    III. Catalytic Activity of Adrenal P450

    IV. Molecular Properties of the Two Cytochrome P450 Proteins

    V. Comparison of Substrate Specificity and Spectral Properties

    VI. Adrenal Steroid Hydroxylases as Second Model P450 Systems


    22 Application of Affinity and Photoaffinity Probes to Cytochromes P450cam and P45011β: New Insights into the Mode of Substrate Binding and Orientation

    I. Introduction

    II. The Substrate-Binding Site of Cytochrome P450cam

    III. The Substrate-Binding Site of Cytochrome P45011β

    IV. Applicability of the Two-Site Hypothesis to P450 Heme Proteins


    23 NADH Peroxidase of Streptococcus faecalis

    I. Oxygen as Electron Acceptor for Streptococcus faecalis

    II. NADH Peroxidase from Fermentatively Grown S. faecalis, 10C1: Demonstration of an Enzyme-Substrate Complex

    III. NADH Peroxidase from Aerobically Grown S. faecalis

    IV. Conclusions


    24 Biochemical Investigations at Subzero Temperatures

    I. Introduction

    II. Temporal Resolution of Enzyme-Catalyzed Reactions

    III. Purification of Reaction Intermediates and Structural Studies


    25 Dynamic Proteins

    Conformational Substates—Concepts and Existence

    Conformational Transitions

    The Spatial Structure of Conformational Substates

    Structure and Function

    Reaction Theory

    A Dynamic Model for Ligand Binding


    V Microbiology

    26 The Microbes' Contribution to Nutritional Science


    Studies on Biotin

    The Microbes' Contribution to Nutritional Science


    27 Pseudomonas aeruginosa—A Highly Evolved Aerobe




Product details

  • No. of pages: 402
  • Language: English
  • Copyright: © Academic Press 1982
  • Published: January 1, 1982
  • Imprint: Academic Press
  • eBook ISBN: 9780323152037

About the Editor

L Ornston

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