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Expansion Microscopy for Cell Biology - 1st Edition - ISBN: 9780128208076

Expansion Microscopy for Cell Biology, Volume 161

1st Edition

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Serial Volume Editors: Paul Guichard Virginie Hamel
Hardcover ISBN: 9780128208076
Imprint: Academic Press
Published Date: 10th January 2021
Page Count: 312
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Table of Contents

1. Protein-retention Expansion Microscopy: Improved Sub-cellular Imaging Resolution through Physical Specimen Expansion
Paul Tilberg
2. Ultrastructure Expansion Microscopy (U-ExM)
Davide Gambarotto, Virginie Hamel and Paul Guichard
3. Expansion STED microscopy (ExSTED)
Helge Ewers
4. Simple multi-color super-resolution by X10 microscopy
Sven Truckenbrodt
5. Expansion microscopy imaging of various neuronal structures
Jae-Byum Chang
6. Mapping the neuronal cytoskeleton using expansion microscopy
Lukas Kapitein
7. Mechanical expansion microscopy
Bo Wang
8. Enhanced expansion microscopy to measure nanoscale structural and biochemical remodelling in single cells
Izzy Jayasinghe
9. Application of Expansion Microscopy on Developing Arabidopsis Seeds
Michael D. Nodine
10. A protocol to expand plant nuclei
Veit Schubert
11. Expansion microscopy of the mitotic spindle
Ivana Ponjavić
12. Expansion microscopy at the nanoscale. The nuclear pore complex as fiducial landmark
Paolo Bianchini
13. Post-labeling expansion microscopy, a promise to go beyond super-resolution limitations
Paul Guichard and Virginie Hamel
14. Ex-dSTORM and automated quantitative image analysis of expanded filamentous structures
Markus Sauer
15. Expansion Microscopy on Drosophila Spermatocyte Centrioles
Alan Wainman


Description

Expansion microscopy for Cell Biology, Volume 161 in the Methods in Cell Biology series, compiles recent developments in expansion microscopy techniques (Pro-ExM, U-ExM, Ex-STED, X10, Ex-dSTORM, etc.) and their applications in cell biology, ranging from mitosis, centrioles or nuclear pore complex to plant cell, bacteria, Drosophila or neurons. Chapters in this new release include Protein-retention Expansion Microscopy: Improved Sub-cellular Imaging Resolution through Physical Specimen Expansion, Ultrastructure Expansion Microscopy (U-ExM), Expansion STED microscopy (ExSTED), Simple multi-color super-resolution by X10 microscopy, Expansion microscopy imaging of various neuronal structures, Mapping the neuronal cytoskeleton using expansion microscopy, Mechanical expansion microscopy, and much more.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Represents the latest release in the Methods in Cell Biology series
  • Includes the latest information on Expansion Microscopy for Cell Biology

Readership

Researchers in the area of Cell biology, Plant biology, Neurobiology as well as other scientific areas


Details

No. of pages:
312
Language:
English
Copyright:
© Academic Press 2021
Published:
10th January 2021
Imprint:
Academic Press
Hardcover ISBN:
9780128208076

Ratings and Reviews


About the Serial Volume Editors

Paul Guichard

Paul Guichard is an expert on the structure of the centriole. From 2007 to 2010, he did his PhD at the Institut Curie (France) under the co-direction of Drs. Tassin and Marco, on the study of the human procentriole assembly using cryo-tomography. From 2011 to 2015, he did his post-doctoral work in the laboratory of Prof. Gönczy at EPFL (Lausanne, Switzerland) where he studied the structure of the centriolar cartwheel by combining cryo-tomography, in vitro assemblies and super-resolution microscopy. In 2015, together with Dr. Hamel, they created the Centriole Architecture Laboratory at the University of Geneva (Switzerland), aiming at revealing the molecular architecture and function of the centriole.

Affiliations and Expertise

Centriole Architecture Laboratory, University of Geneva, Switzerland

Virginie Hamel

Virginie Hamel (previously Hachet) is an expert in Cell Biology and Biochemistry, mainly focusing on understanding the function of the centriole and centrosome. She completed her PhD under the supervision of Iain Mattaj at EMBL (Heidelberg, Germany, 2004) working on the role of importin  in nuclear envelope re-assembly in vitro using Xenopus laevis egg extracts. She then carried out postdoctoral studies in the laboratory of Prof. Gönczy at ISREC/EPFL (Lausanne, Switzerland) from 2005 to 2012, where she became interested in the mechanisms dictating timing of mitotic entry in C. elegans embryos. She next was appointed scientist collaborator in that lab (2013-2015) and started to work on centriole biology. She teamed up with Paul Guichard to specifically dissect the mechanisms of centriole assembly. Since 2015, she is scientific collaborator co-heading the Centriole architecture lab with Prof Paul Guichard in the Cell Biology Department at the University of Geneva (Switzerland). Their lab focuses on deciphering the structural mechanisms governing centriole assembly. To this end, they are combining the use of cell biology methods, in vitro reconstitution assays, cryo-microscopy/cryo-tomography and super-resolution microscopy including expansion microscopy to gain decisive insights into these mechanisms.

Affiliations and Expertise

Centriole Architecture Laboratory, University of Geneva, Switzerland