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Eukaryotic RNases and their Partners in RNA Degradation and Biogenesis - 1st Edition - ISBN: 9780124047419, 9780124047440

Eukaryotic RNases and their Partners in RNA Degradation and Biogenesis, Volume 32

1st Edition

Part B

Serial Editor: Fuyuhiko Tamanoi
Series Volume Editor: Fuyuhiko Tamanoi
Serial Volume Editor: Feng Guo
Hardcover ISBN: 9780124047419
eBook ISBN: 9780124047440
Imprint: Academic Press
Published Date: 27th November 2012
Page Count: 254
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Table of Contents


Chapter One. Dicer Proteins and Their Role in Gene Silencing Pathways

1. Introduction

2. Dicers: RNase III Family Members with a Role in Gene Silencing

3. Mechanism of Processing and the Role of the Catalytic RNase III Domains

4. Role of Other Domains in Dicer Function

5. Domain Arrangement in Metazoan Dicers

6. Inside-Out Processing Mechanism by the Yeast Dicer

7. Function of Dicer in the Nucleus

8. Proteins Interacting with Dicer and the Assembly of RISC Effector Complexes

9. Future Perspectives


Chapter Two. Loquacious, a Dicer Partner Protein, Functions in Both the MicroRNA and siRNA Pathways

1. Introduction

2. miRNA and siRNA Pathways in the Fruit Fly Drosophila melanogaster

3. Drosophila Dicer-1 and Dicer-2: Similar but Distinct Domain Structures

4. Conserved Partnerships Between Dicer and Dicer-Binding Partner dsRBD Proteins

5. loqs Encodes Four Alternatively Spliced Isoforms

6. Loqs-PA and Loqs-PB Bind to Dicer-1, While Loqs-PD and R2D2 Bind to Dicer-2

7. Partner Protein Mutant Phenotypes in Flies and Mammals

8. Loqs-PA and Loqs-PB Function in the miRNA Pathway

9. Loqs-PD Functions in the siRNA Pathway

10. Loqs-PB Is Required to Maintain Ovarian Germline Stem Cells

11. For Some miRNAs, Loqs-PB Tunes Mature miRNA Length

12. Future Directions

13. Concluding Remarks


Chapter Three. Translin: TRAX Complex (C3PO), a Novel Ribonuclease for the Degradation of Small RNAs

1. Introduction: Earlier Studies of Translin and TRAX

2. Discovery of C3PO as a Novel Endoribonuclease Involved in the Activation of RNA-Induced Silencing Complex

3. C3PO Ribonuclease in tRNA Processing

4. Structure of C3PO: An Unusual Asymmetrical Hetero-Octamer

5. Oligomeric States of C3PO and Implications for Substrate Binding and Activation

6. Enzymatic Properties of C3PO

7. Concluding Remarks


Chapter Four. Structure and Mechanism of Argonaute Proteins

1. Introduction

2. Basic Argonaute Architecture

3. Recognition of the Guide 5′-end by the MID Domain

4. Preorganization of the Seed Facilitates Target Recognition

5. Recognition of the Guide RNA 3′-end by the PAZ Domain

6. The Two-State Model of Target Binding

7. The Rubber Band Model of RISC Loading

8. The Slicer Active Site Resides in the PIWI Domain

9. Recruitment of Silencing Factors


Chapter Five. Drosha and DGCR8 in MicroRNA Biogenesis

1. Introduction

2. Drosha

3. DGCR8 as the RNA-Binding Partner of Drosha

4. DGCR8 as a Heme Protein

5. DGCR8 in DiGeorge Syndrome

6. How do DGCR8 and Drosha Structurally Recognize Pri-miRNAs?

7. How Is Pri-miRNA Processing Regulated?

8. Drosha and DGCR8 Bind RNAs Other Than Pri-miRNAs

9. Future Perspectives


Chapter Six. Control of Drosha-Mediated MicroRNA Maturation by Smad Proteins

1. miRNA Biogenesis

2. Drosha and DGCR8

3. Modulators of the Drosha Microprocessor Complex

4. Smad Proteins

5. miRNA Regulation by Smads

6. Closing Remark


Chapter Seven. PIWI Proteins and Their Slicer Activity in piRNA Biogenesis and Transposon Silencing

1. Introduction

2. Drosophila PIWI Proteins

3. piRNA Biogenesis

4. Outstanding Questions


Chapter Eight. Control of MicroRNA Maturation by p53 Tumor Suppressor and MCPIP1 Ribonuclease

1. Introduction

2. Two-Step Endoribonuclease Reactions in miRNA Maturation

3. Control of miRNA Biosynthesis by p53

4. Negative Regulation of miRNA Processing by MCPIP1

5. Autoregulation of miRNA Processing Processes

6. Alteration of miRNA Biogenesis in Human Cancer

7. Conclusion


Chapter Nine. Nanoparticle-Based Delivery of siRNA and miRNA for Cancer Therapy

1. Introduction

2. Potential Use of siRNAs and miRNAs for Cancer Therapy

3. Nanoparticle-Based Delivery

4. Summary and Future Prospects


Author Index

Subject Index


This special volume of The Enzymes is targeted towards researchers in biochemistry, molecular and cell biology, pharmacology, and cancer. This thematic volume discusses Eukaryotic RNases and their partners in RNA degradation and biogenesis.

Key Features

  • Contributions from leading authorities
  • Informs and updates on all the latest developments in the field


Researchers in biochemistry, molecular and cell biology, pharmacology, and cancer


No. of pages:
© Academic Press 2012
27th November 2012
Academic Press
Hardcover ISBN:
eBook ISBN:

Ratings and Reviews

About the Serial Editor

Fuyuhiko Tamanoi

Affiliations and Expertise

Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, USA

About the Series Volume Editor

Fuyuhiko Tamanoi

Affiliations and Expertise

Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, USA

About the Serial Volume Editor

Feng Guo