Eukaryotic Cell Genetics

Eukaryotic Cell Genetics

1st Edition - January 28, 1983

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  • Author: John Morrow
  • eBook ISBN: 9780323158183

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Description

Eukaryotic Cell Genetics reviews the state of knowledge in somatic cell genetics. The book begins by discussing the development of somatic cell genetics, focusing on the estimation of mutation rates in mammalian cells, with frequent reference to the use of drug resistance as a selective character. It then considers some of the specific properties of such variants in order to understand their molecular basis. The subsequent chapters examine the properties of specific types of auxotrophic variants; the means by which eukaryotic cells may be reassembled to give rise to viable cellular composites; gene regulation in eukaryotic organisms; and chromosome mapping. The discussions also include differentiation in cultured cells; neoplastic transformation; the modulation of gene expression in cultured cells; mutation induction in cultured cells; applications of cell culture; and the mechanism of cellular aging. This book is intended for researchers in the fields of genetics and molecular biology, nonspecialists interested in what is happening in a very exciting area of biology, and students at the graduate level in cell biology.

Table of Contents


  • Preface

    1 Somatic Cell Genetics and the Legacy of Microbial Systems

    I. Introduction

    II. Mutation versus Adaptation in Bacterial Populations

    III. The Basis of Variation in Somatic Cells

    IV. Summary and Conclusions

    2 Drug Resistance and Its Genetic Basis

    I. Introduction

    II. Purine Analogs

    III. Pyrimidine Analogs

    IV. Drugs Other than Purine and Pyrimidine Analogs

    V. Conclusions

    3 Auxotrophic Variants in Cultured Cells

    I. Introduction

    II. Techniques for Isolation

    III. Properties of Variants

    IV. Conclusions

    4 Mechanisms for the Exchange of Genetic Information in Cultured Cells

    I. Introduction

    II. Cell Hybridization

    III. Genetic Exchange Using Cell Components

    IV. Transfer of Genetic Information Using Purified Metaphase Chromosomes

    V. Transformation Using Purified DNA Preparations

    VI. Transfer of Information through Direct Microinjection of RNA and DNA

    VII. Conclusion

    5 The Regulation of Gene Expression in Heterokaryons

    I. Introduction

    II. Nuclear Reactivation in Heterokaryons

    III. The Expression of Genetic Information in Heterokaryons

    IV. The Mechanism of Nuclear Activation in Heterokaryons

    V. Conclusions

    6 Chromosome Mapping

    I. Introduction

    II. Mapping by Pedigree Analysis

    III. Linkage Mapping Using Somatic Cell Hybridization, Chromosomal Variants, and Nucleic Acid Hybridization

    IV. Conclusions

    7 Differentiation in Cultured Cells: Liver Cells

    I. Introduction

    II. Liver Cells and Their Hybrids in the Study of Differentiation

    III. Clonal Variation in Liver Cells

    IV. Transfer of Control Factors via Cytoplasms

    V. Conclusion

    8 Differentiation in Cultured Cells: Muscle Cells, Melanoma Cells, Neuronal Cells, and Hemoglobin- Producing Cells

    I. Introduction

    II. Muscle

    III. Melanin Synthesis in Cell Hybrids

    IV. Nerve Cells

    V. Hemoglobin Synthesis in Cell Hybrids

    VI. Conclusion and Summary

    9 Differentiation in Cultured Cells: Cells of the Immune System

    I. Introduction

    II. Mechanism of Antibody Diversity

    III. The Structure of the Antibody Molecule

    IV. The Arrangement of Antibody Genes

    V. Immunoglobulin Expression in Cell Hybrids

    VI. Monoclonat Antibodies Produced by Cell Hybrids

    VII. Conclusion

    10 Hypotheses of Malignancy and Their Analysis through the Use of Somatic Cell Hybrids

    I. Introduction

    II. Models of Cancer

    III. Analysis of Malignancy in Intraspecific Somatic Cell Hybrids

    IV. Genetic Control of Malignancy in Interspecific Hybrids

    V. Chromosomal Alterations and Malignancy

    VI. Conclusions

    11 Modulation of Gene Expression in Cultured Cells

    I. Introduction

    II. Response to Steroid Hormones in Eukaryotic Cells

    III. Effects of Bromodeoxyuridine on Differentiated Gene Functions

    IV. Cyclic AMP Response in Cultured Cells

    V. Conclusions

    12 Mutation Induction in Cultured Cells

    I. Introduction

    II. Problems Involved in Mutagenesis Studies

    III. Induction of Mutations by X Rays and Nonionizing Radiation

    IV. Chemical Mutagenesis

    V. Conclusions

    13 Use of Cell Culture in the Analysis of Human Heredity

    I. Introduction

    II. Hypercholesterolemia

    III. X Chromosomal Inactivation

    IV. Xeroderma Pigmentosum

    V. Testicular Feminization

    VI. Conclusions

    14 The Cellular Basis of the Aging Process

    I. Introduction

    II. The Aging of Diploid Fibroblasts in Vitro

    III. Proliferative Capacity of Diploid Fibroblasts and the Normal Aging Process

    IV. Error Catastrophe Hypothesis

    V. Conclusions

    15 Future Outlook

    I. Introduction

    II. Recombinant DNA Technology

    III. Immunogenetics

    IV. Human Genetics

    V. Aging

    VI. Malignancy

    VII. Differentiation

    VIII. Conclusions

    Bibliography

    Index






Product details

  • No. of pages: 276
  • Language: English
  • Copyright: © Academic Press 1983
  • Published: January 28, 1983
  • Imprint: Academic Press
  • eBook ISBN: 9780323158183

About the Author

John Morrow

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