Essentials in Modern HPLC Separations

Essentials in Modern HPLC Separations

2nd Edition - June 24, 2022

Write a review

  • Authors: Serban Moldoveanu, Victor David
  • Paperback ISBN: 9780323911771
  • eBook ISBN: 9780323998369

Purchase options

Purchase options
DRM-free (EPub, PDF)
Sales tax will be calculated at check-out

Institutional Subscription

Free Global Shipping
No minimum order


Essentials in Modern HPLC Separations, Second Edition discusses the role of separation in high performance liquid chromatography (HPLC). This new and updated edition systematically presents basic concepts as well as new developments in HPLC. Starting with a description of basic concepts, it provides important guidance for the practical utilization of various HPLC procedures, such as the selection of the HPLC type, proper choice of the chromatographic column, selection of mobile phase and selection of the method of detection, all of which are in correlation with the physico-chemical characteristics of the compounds separated. Every chapter has been carefully reviewed, with several new sections added to bring the book completely up-to-date. Hence, it is a valuable reference for students and professors in chemistry.

Key Features

  • Provides a thoroughly updated resource, with an entirely new section on Computer-aided Method Development in HPLC and new subsections on miniaturization and automation in HPLC, chemometric aspects of HPLC, green solvent use in HPLC, and more
  • Includes insights into the chromatographic process to find the optimum solution for analyzing complex samples
  • Presents a basis for understanding the utilization of modern HPLC for applications, particularly for the analysis of pharmaceutical, biological, food, beverage and environmental samples


Chemists of a wide range of levels of specialization, from industry, research and academic sectors; students and professors in PhD and Masters programs in chemistry

Table of Contents

  • Cover image
  • Title page
  • Table of Contents
  • Copyright
  • Preface
  • Section I. Basic information about HPLC
  • Chapter 1. Introductory information regarding HPLC
  • 1.1. Preliminary discussion about HPLC
  • 1.2. Main types of HPLC
  • 1.3. Flow of a typical HPLC analysis
  • Chapter 2. Overview of HPLC instrumentation and its use
  • 2.1. Description of main components of HPLC instrumentation
  • Chapter 3. Parameters for the characterization of HPLC separation
  • 3.1. Parameters describing the chromatographic peak
  • 3.2. Parameters describing the separation
  • 3.3. Summary of chromatographic peak and separation characteristics
  • 3.4. Parameters related to quantitation in HPLC
  • 3.5. Parameters characterizing the gradient separation
  • Chapter 4. Equilibrium types in HPLC
  • 4.1. Partition equilibrium
  • 4.2. Adsorption equilibrium
  • 4.3. Equilibrium involving ions
  • 4.4. Equilibrium in size exclusion processes
  • 4.5. The influence of pH and of additives on retention equilibria
  • 4.6. The influence of temperature on retention equilibrium
  • 4.7. Reactions occurring in HPLC column
  • Chapter 5. Intermolecular interactions
  • 5.1. Forces between molecules
  • 5.2. Forces between molecules and a charged surface
  • Chapter 6. Characterization of analytes and matrices
  • 6.1. Properties of solutes important for HPLC separation
  • 6.2. Physico-chemical properties related to detection
  • 6.3. Properties of matrix related to HPLC separation and detection
  • Chapter 7. Mobile phases and their properties
  • 7.1. Characterization of liquids as eluents in HPLC
  • 7.2. Additional physical properties of liquids affecting separation
  • 7.3. Properties of liquids affecting HPLC detection
  • 7.4. Buffers and additives
  • 7.5. General use of solvents as mobile phase
  • 7.6. Solvents for sample injection and for needle wash
  • 7.7. Gradient elution
  • Chapter 8. Analytical HPLC columns and their characteristics
  • 8.1. Construction of an HPLC column
  • 8.2. Column properties affecting separation
  • 8.3. Selection of a column for an HPLC separation
  • Section II. Main types of HPLC separations
  • Chapter 9. Reversed-phase HPLC
  • 9.1. Retention and elution process in RP-HPLC
  • 9.2. Stationary phases and columns for RP-HPLC
  • 9.3. Parameters used for the characterization of RP-HPLC phases and columns
  • 9.4. Selection of the column in RP-HPLC
  • 9.5. Mobile phases in reversed-phase liquid chromatography
  • 9.6. Selection of mobile phase in RP-HPLC
  • 9.7. Prediction of parameters describing the separation in RP-HPLC
  • Chapter 10. Other HPLC separations performed on hydrophobic stationary phases
  • 10.1. Nonaqueous RP-HPLC
  • 10.2. Ion pair liquid chromatography
  • 10.3. Hydrophobic interaction chromatography (HIC)
  • 10.4. Micellar, microemulsion, and other types of HPLC performed on hydrophobic phases
  • Chapter 11. Hydrophilic interaction liquid chromatography
  • 11.1. Retention and elution process in HILIC
  • 11.2. Polar stationary phases and columns
  • 11.3. Retention and separation properties of polar stationary phases
  • 11.4. Selection of the column in HILIC separations
  • 11.5. Mobile phase in HILIC
  • 11.6. Prediction of parameters describing the separation in HILIC
  • Chapter 12. Other HPLC separations performed on polar stationary phases
  • 12.1. Normal phase liquid chromatography
  • 12.2. Other chromatographic techniques based on polar interactions
  • Chapter 13. Ion exchange, ion-moderated, and ligand exchange liquid chromatography
  • 13.1. Retention and elution in ion exchange liquid chromatography
  • 13.2. Stationary phases and columns for ion exchange and related HPLC techniques
  • 13.3. Characterization of ion exchange stationary phases
  • 13.4. Selection of an ion exchange stationary phase
  • 13.5. Mobile phase in ion exchange and ion-moderated liquid chromatography
  • Chapter 14. Chiral HPLC separations
  • 14.1. Separation process in chiral liquid chromatography
  • 14.2. Stationary phases and columns for chiral separations
  • 14.3. Characterization of chiral stationary phases
  • 14.4. Selection of a chiral stationary phase
  • 14.5. Mobile phase in chiral HPLC
  • Chapter 15. Size exclusion HPLC
  • 15.1. Separation process in size exclusion chromatography
  • 15.2. Stationary phases and columns for size exclusion HPLC
  • 15.3. Characterization of size exclusion phases and columns
  • 15.4. Selection of a stationary phase in size exclusion HPLC
  • 15.5. Mobile phase for size exclusion separations
  • 15.6. Interaction polymer chromatography
  • Chapter 16. Affinity, immunoaffinity, and aptamer type HPLC
  • 16.1. Separation process in immunoaffinity HPLC
  • 16.2. Types of phases and their preparation in affinity and immunoaffinity chromatography
  • 16.3. Biomimetic liquid chromatography
  • Chapter 17. Mixed-mode HPLC
  • 17.1. Stationary phases with more than one type of active groups
  • 17.2. Mobile phase in mixed-mode HPLC
  • Section III. Practice of HPLC analysis
  • Chapter 18. Utilization of HPLC in chemical analysis
  • 18.1. Steps in development and implementation of an HPLC separation
  • 18.2. Application of HPLC for quantitative analysis
  • Appendix to Chapter 6
  • Appendix to chapter 7
  • Appendix to chapter 8
  • Appendix to chapter 9
  • Appendix to chapter 11
  • Appendix to chapter 13
  • Appendix to chapter 14
  • Appendix to chapter 15
  • Index

Product details

  • No. of pages: 716
  • Language: English
  • Copyright: © Elsevier 2022
  • Published: June 24, 2022
  • Imprint: Elsevier
  • Paperback ISBN: 9780323911771
  • eBook ISBN: 9780323998369

About the Authors

Serban Moldoveanu

Serban Moldoveanu
Dr. Serban C. Moldoveanu is Senior Principal Scientist at R. J. Reynolds Tobacco Company. His research activity is focused on various aspects of chromatography including method development for the analysis by GC/MS, HPLC, and LC/MS/MS of natural products and cigarette smoke. He has also performed research on pyrolysis of a variety of polymers and small molecules. He has over 100 publications in peer reviewed journals, eleven books, and several chapter contributions. He is a member of the editorial board of the Journal of Analytical Methods in Chemistry.

Affiliations and Expertise

Senior Principal Scientist, RJ Reynolds Tobacco Co., Winston-Salem, NC, USA

Victor David

Victor David
Dr. Victor David is a Professor and Head of the Department of Analytical Chemistry, University of Bucharest, Romania. He is the author of more than 120 publications, including 92 scientific papers in ISI international journals and 10 books and chapters in various Encyclopedias. He is also a reviewer at several international journals.

Affiliations and Expertise

Professor and Head of the Department of Analytical Chemistry, University of Bucharest, Romania

Ratings and Reviews

Write a review

There are currently no reviews for "Essentials in Modern HPLC Separations"