This is a fast-moving field, and these detailed methods will help drive advances in stem cell research. The editors have hand selected step-by-step methods from researchers with extensive reputations and expertise. This volume, as part of the Reliable Lab Solutions series, delivers busy researchers a handy, time-saving source for the best methods and protocols in stem cells.

Key Features

* Provides powerful research opportunities for those interested in perusing work in pluripotent stem cells, disease modeling, and other aspects of basic stem cell research * Refines, organizes and updates popular methods from flagship series, Methods in Enzymology *Highlights top downloads, enhanced with author tips and tricks and pitfalls to avoid


Researchers in biochemistry, cell and molecular biology, developmental biology and genetics.

Table of Contents

Section 1: Organ-Derived Stem Cells 1. Neural Stem Cell Isolation and Characterization Rodney L. Rietze and Brent A. Reynolds 2. Neural Stem Cells and Their Manipulation Evan Snyder 3. Retinal Stem Cells Thomas A. Reh and Andy J. Fischer 4. Dental Pulp Stem Cells He Liu, Stan Gronthos and Songtao Shi 5. Spermatogonial Stem Cells Jon M. Oatley and Ralph L. Brinster 6. Stem Cells in the Lung Xiaoming Liu, Ryan R. Driskell and John F. Engelhardt 7. Pancreatic Cells and Their Progenitors Seth J. Salpeter and Yuval Dor 8. Pluripotent Stem Cells from Germ Cells Candace L. Kerr, Michael J. Shamblott and John D. Gearhart 9. Amniotic Fluid and Placental Stem Cells Dawn M. Delo, Paolo De Coppi, Georg Bartsch, Jr. and Anthony Atala 10. Cord Blood Stem and Progenitor Cells Mervin C. Yoder 11. Purification of Hematopoietic Stem Cells Using the Side Population K.K. Lin and Margaret A. Goodell 12. Microarray Analysis of Stem Cells and Differentiation Howard Y. Chang 13. Tissue Engineering Using Adult Stem Cells Daniel Eberli 14. Mesenchymal Stem Cells and Tissue Engineering Nicholas W. Marion and Jeremy J. Mao Section II: Embryonic Stem Cells 15. Murine Embryonic Stem Cells Andras Nagy and Kristina Vintersten 16. Human Embryo Culture Amparo Mercader, Diana Valbuena and Carlos Simón 17. Human Embryonic Stem Cells Douglas Melton 18. Characterization and Evaluation of Human Embryonic Stem Cells Chunhui Xu 19. Feeder-Free Culture of Human Embryonic Stem Cells Michal Amit and


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About the editors

Robert Lanza

Robert Lanza, M.D. is currently Chief Scientific Officer at Advanced Cell Technology, and Adjunct Professor of Surgical Sciences at Wake Forest University School of Medicine. He has several hundred scientific publications and patents, and over 30 books, including Principles of Tissue Engineering (1st through 4th Editions), Methods of Tissue Engineering, Principles of Cloning (1st and 2nd Editions), Essentials of Stem Cell Biology (1st and 2nd Editions), XENO, Yearbook of Cell & Tissue Transplantation, One World: The Health & Survival of the Human Species in the 21st Century (as editor, with forewords by C. Everett Koop and former President Jimmy Carter), and Medical Science & the Advancement of World Health. Dr. Lanza received his B.A. and M.D. degrees from the University of Pennsylvania, where he was both a University Scholar and Benjamin Franklin Scholar. He is a former Fulbright Scholar, and studied as a student in the laboratory of Richard Hynes (MIT), Jonas Salk (The Salk Institute), and Nobel laureates Gerald Edelman (Rockefeller University) and Rodney Porter (Oxford University). He also worked closely (and coauthored a series of papers) with the late Harvard psychologist B.F. Skinner and heart transplant pioneer Christiaan Barnard. Dr. Lanza's current area of research focuses on the use of stem cells in regenerative medicine.

Affiliations and Expertise

Advanced Cell Technology, MA, USA

Irina Klimanskaya

Affiliations and Expertise

Advance Cell Technology, Worcester, MA, USA