Essential Stem Cell Methods

Essential Stem Cell Methods

1st Edition - December 12, 2008

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  • Editors: Robert Lanza, Irina Klimanskaya
  • eBook ISBN: 9780080885254

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This is a fast-moving field, and these detailed methods will help drive advances in stem cell research. The editors have hand selected step-by-step methods from researchers with extensive reputations and expertise.This volume, as part of the Reliable Lab Solutions series, delivers busy researchers a handy, time-saving source for the best methods and protocols in stem cells.

Key Features

* Provides powerful research opportunities for those interested in perusing work in pluripotent stem cells, disease modeling, and other aspects of basic stem cell research

* Refines, organizes and updates popular methods from flagship series, Methods in Enzymology

*Highlights top downloads, enhanced with author tips and tricks and pitfalls to avoid


Researchers in biochemistry, cell and molecular biology, developmental biology and genetics.

Table of Contents

  • Section 1: Organ-Derived Stem Cells

    1. Neural Stem Cell Isolation and Characterization
    Rodney L. Rietze and Brent A. Reynolds
    2. Neural Stem Cells and Their Manipulation Evan Snyder
    3. Retinal Stem Cells
    Thomas A. Reh and Andy J. Fischer
    4. Dental Pulp Stem Cells
    He Liu, Stan Gronthos and Songtao Shi
    5. Spermatogonial Stem Cells
    Jon M. Oatley and Ralph L. Brinster
    6. Stem Cells in the Lung
    Xiaoming Liu, Ryan R. Driskell and John F. Engelhardt
    7. Pancreatic Cells and Their Progenitors
    Seth J. Salpeter and Yuval Dor
    8. Pluripotent Stem Cells from Germ Cells Candace L. Kerr, Michael J. Shamblott and John D. Gearhart
    9. Amniotic Fluid and Placental Stem Cells Dawn M. Delo, Paolo De Coppi, Georg Bartsch, Jr. and Anthony Atala
    10. Cord Blood Stem and Progenitor Cells Mervin C. Yoder
    11. Purification of Hematopoietic Stem Cells Using the Side Population
    K.K. Lin and Margaret A. Goodell
    12. Microarray Analysis of Stem Cells and Differentiation
    Howard Y. Chang
    13. Tissue Engineering Using Adult Stem Cells Daniel Eberli
    14. Mesenchymal Stem Cells and Tissue Engineering
    Nicholas W. Marion and Jeremy J. Mao

    Section II: Embryonic Stem Cells

    15. Murine Embryonic Stem Cells
    Andras Nagy and Kristina Vintersten
    16. Human Embryo Culture
    Amparo Mercader, Diana Valbuena and Carlos Simón
    17. Human Embryonic Stem Cells
    Douglas Melton
    18. Characterization and Evaluation of Human Embryonic Stem Cells
    Chunhui Xu
    19. Feeder-Free Culture of Human Embryonic Stem Cells
    Michal Amit and Joseph Itskovitz-Eldor
    20. Neural Stem Cells, Neurons, and Glia
    Steven M. Pollard, Alex Benchoua and Sally Lowell
    21. Hematopoietic Cells
    Malcolm A.S. Moore, Jae-Hung Shieh and Gabsang Lee
    22. Cardiomyocytes
    Xiangzhong Yang, Xi-Min Guo, Chang-Yong Wang and X. Cindy Tian
    23. Insulin-Producing Cells
    Insa S. Schroeder, Gabriela Kania, Przemyslaw Blyszczuk
    24. Transgene Expression and RNA Interference in Embryonic Stem Cells
    Holm Zaehres and George Q. Daley
    25. Lentiviral Vector-Mediated Gene Delivery into Human Embryonic Stem Cells
    Michal Gropp and Benjamin Reubinoff
    26. Engineering Embryonic Stem Cells with Recombinase Systems
    Frank Schnütgen, A. Francis Stewart, Harald von Melchner and Konstantinos Anastassiadis
    27. Tissue Engineering Using Embryonic Stem Cells Shahar Cohen, Lucy Leshanski and Joseph Itskovitz-Eldor

Product details

  • No. of pages: 628
  • Language: English
  • Copyright: © Academic Press 2008
  • Published: December 12, 2008
  • Imprint: Academic Press
  • eBook ISBN: 9780080885254

About the Editors

Robert Lanza

Robert Lanza
Robert Lanza, M.D. is currently Head of Astellas Global Regenerative Medicine, Chief Scientific Officer of AIRM and an adjunct professor at the Wake Forest Institute for Regenerative Medicine. Time magazine recognized him as one of the “100 Most Influential People in the World,” and Prospect magazine named him one of the Top 50 “World Thinkers.” His research focuses on stem cells and their potential to provide therapies for some of the world's most deadly and debilitating conditions. He has hundreds of scientific publications and over 30 books, including definitive references in the fields of tissue engineering and regenerative medicine. He is a former Fulbright Scholar, and studied with polio-pioneer Jonas Salk and Nobel laureates Gerald Edelman and Rodney Porter. He also worked closely (and co-authored a series of papers) with psychologist BF Skinner and heart transplant-pioneer Christiaan Barnard. Dr. Lanza received his undergraduate and medical degrees from the University of Pennsylvania, where he was both a University Scholar and Benjamin Franklin Scholar. Lanza was part of the team that cloned the world’s first human embryo, the first endangered species, and published the first-ever reports of pluripotent stem cell use in humans.

Affiliations and Expertise

Astellas Institute for Regenerative Medicine, Westborough, MA, USA

Irina Klimanskaya

Affiliations and Expertise

Advance Cell Technology, Worcester, MA, USA

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