
Essential Ion Channel Methods
Description
Key Features
- Highlights top downloaded and cited chapters, authored by pioneers in the field and enhanced with graphics and easy to follow methods
- Loaded with detailed protocols developed and used by leaders in the field
- Refines, organizes and updates popular methods from one of our top selling series, Methods in Enzymology
Readership
Table of Contents
PART I - Assembly
1. Assembly of Ion Channels
ZuFang Sheng and Carol DeutschPART II - Genetics
2. Identification of Ion Channel-Associated Proteins Using the Yeast Two-Hybrid System
Martin Niethammer and Morgan ShengPART III - Electrophysiology
3. Patch Clamp Studies of the Cystic Fibrosis Transmembrane Conductance Regulator Chloride Channel
John W. Hanrahan, Zie Kone , Ceri J. Mathews,Jiexin Luo, Yanlin Jia and Paul Lindsell4. Tight-Seal Whole-cell Patch Clamping of C. elegans Neurons
S.R. Lockery and M.B. Goodman5. Gating Currents
Enrico Stefani and Francisco Bezanilla6. Determining Ion Channel Permeation Properties
Ted BegenisichPART IV - Expression Systems
7. Expression of Ligand-Gated Ion Channels Using Using Semliki Forest Virus and Baculovirus
Kathryn Radford and Gary Buell8. Recombinant Adenovirus-Mediated Expression in Nervous System of Genes Coding for Ion Channels and Other Molecules Involved in Synaptic Function
Markus U. Ehrengruber, Markus Lanzrein, Youfeng Xu, Mark C. Jasek, David B. Kantor, Erin M. Schuman, Henry A. Lester, and Norman Davidson9. Transient Expression of Heteromeric Ion Channels
Alison L. Eertmoed, Yolanda F. Vallejo, and William N. GreenPART V - Model Simulations
10. Computer Simulations and Modeling of Ion Channels
Michael E. GreenPART VI. Physical
11. Fluorescence Techniques for Measuring Ion Channel Activity
Gönül Velicelebi, Kenneth A. Stauderman, Mark A. Varney, Michael Akong, Stephen D. Hess, and Edwin C. Johnson12. Ligand Binding Methods for Analysis of Ion Channel Structure and Function
Steen E. Pedersen, Monica M. Lurtz, and Rao V. L. Papineni13. Three-dimensional structure of membrane proteins determined by two-dimensional crystallization, electron cryomicroscopy, and image analysis
Mark Yeager, Vinzenz M. Unger, Alok K. Mitra14. Voltage Clamp Biosensors for Capillary Electrophoresis
Owe Orwar, Kent Jardemark, Cecilia Farre, Ingemar Jacobson, Alexander Moscho, Jason B. Shear, Harvey A. Fishman, Sheri J. Lillard, Richard N. Zare15. Ion Channels As Tools to Monitor Lipid Bilayer-Membrane Protein Interactions: Gramicidin Channels As Molecular Force
O. S. Andersen, C. Nielsen, A. M. Maer, J. A. Lundbæk, M. Goulian, and R. E. Koeppe IPART VII. Purification and Reconstitution
16. Purification and Reconstitution of the Epithelial Chloride Channel Cystic Fibrosis Transmembrane Conductance Regulator
Mohabir Ramjeesingh, Elizabeth Garani, Kevin Galley, Canhui Li, Yanchun Wang, Christine E. Bear17. Reconstitution of Native and Cloned Channels into Planar Bilayers
I. Favre, Y.-M. Sun, and E. MoczydlowskiPART VIII. Second Messengers and Biochemical Approaches
18. Protein Phosphorylation of Ligand-Gated Ion Channels
Andrew l. Mammen, Sunjeev Kamboj, and Richard L. Huganir19. Analysis of Ion Channel Associated Proteins
Michael Wyszynski and Morgan Sheng20. Secondary Messenger Regulation of Ion Channels/Plant Patch Clamping
Sarah M. Assmann and Lisa RomanoPART IX. Special Channels
21. ATP-Sensitive Potassium Channels
Joseph Bryan, M. Schwanstecher, C. Schwanstecher, F. Chudziak, U. Panten, J.P. Clement IV, G. Gonzalez, and L. Aguilar-Bryan22. Simplified Fast Pressure-Clamp Technique for Studying Mechanically-Gated Channels
Don W. McBride, Jr. and Owen P. Hamill23. Purification and Heterologous Expression of Inhibitory Glycine Receptors
Bodo Laube and Heinrich Betz24. Functional Analyses of Aquaporin Water Channel Proteins
Peter Agre, John C. Mathai, Barbara L. Smith, and Gregory M. PrestonPART X. Toxins and Other Membrane Active Compounds
25. Conus Peptides as Probes for Ion Channels
J. Michael McIntosh, Baldomero M. Olivera, and Lourdes J. Cruz26. Scorpion Toxins as Tools for Studying Potassium Channels
Maria Garcia, Markus Hanner, Hans-Günther Knaus, Robert Slaughter, Gregory J. Kaczorowski27. The Use of Planar Lipid Bilayer Membranes for Rapid Screening of Membrane Active Compounds
Bruce L. Kagan, Tajb A. Mirzabekov, Antoly Y. Silberstein28. Antibodies to Ion Channels
Angela Vincent, Ian Hart, Ashwin Pinto, and F. Anne Stephenson
Product details
- No. of pages: 632
- Language: English
- Copyright: © Academic Press 2010
- Published: June 14, 2010
- Imprint: Academic Press
- eBook ISBN: 9780123822376