Description

In recent years, there have been considerable developments in techniques for the investigation and utilisation of enzymes. With the assistance of a co-author, this popular student textbook has been updated to include techniques such as membrane chromatography, aqueous phase partitioning, engineering recombinant proteins for purification and due to the rapid advances in bioinformatics/proteomics, a discussion of the analysis of complex protein mixtures by 2D-electrophoresis and RPHPLC prior to sequencing by mass spectroscopy. Written with the student firmly in mind, no previous knowledge of biochemistry, and little of chemistry, is assumed. It is intended to provide an introduction to enzymology, and a balanced account of all the various theoretical and applied aspects of the subject which are likely to be included in a course.

Key Features

  • Provides an introduction to enzymology and a balanced account of the theoretical and applied aspects of the subject
  • Discusses techniques such as membrane chromatography, aqueous phase partitioning and engineering recombinant proteins for purification
  • Includes a discussion of the analysis of complex protein mixtures by 2D-electrophoresis and RPHPLC prior to sequencing by mass spectroscopy

Readership

Undergraduate students

Table of Contents

  • Authors’ Preface
  • Part 1: Structure and Function of Enzymes
    • 1: An Introduction to Enzymes
      • 1.1 WHAT ARE ENZYMES?
      • 1.2 A BRIEF HISTORY OF ENZYMES
      • 1.3 THE NAMING AND CLASSIFICATION OF ENZYMES
      • SUMMARY OF CHAPTER 1
      • PROBLEMS
    • 2: The Structure of Proteins
      • 2.1 INTRODUCTION
      • 2.2 AMINO ACIDS, THE BUILDING BLOCKS OF PROTEINS
      • 2.3 THE BASIS OF PROTEIN STRUCTURE
      • 2.4 THE DETERMINATION OF PRIMARY STRUCTURE
      • 2.5 THE DETERMINATION OF PROTEIN STRUCTURE BY X-RAY CRYSTALLOGRAPHY
      • 2.6 THE INVESTIGATION OF PROTEIN STRUCTURE IN SOLUTION
      • SUMMARY OF CHAPTER 2
      • PROBLEMS
    • 3: The Biosynthesis and Properties of Proteins
      • 3.1 THE BIOSYNTHESIS OF PROTEINS
      • 3.2 THE PROPERTIES OF PROTEINS
      • SUMMARY OF CHAPTER 3
      • PROBLEMS
    • 4: Specificity of Enzyme Action
      • 4.1 TYPES OF SPECIFICITY
      • 4.2 THE ACTIVE SITE
      • 4.3 THE FISCHER ‘LOCK-AND-KEY’ HYPOTHESIS
      • 4.4 THE KOSHLAND ‘INDUCED-FIT’ HYPOTHESIS
      • 4.5 HYPOTHESES INVOLVING STRAIN OR TRANSITION-STATE STABILIZATION
      • 4.6 FURTHER COMMENTS ON SPECIFICITY
      • SUMMARY OF CHAPTER 4
    • 5: Monomeric and Oligomeric Enzymes
      • 5.1 MONOMERIC ENZYMES
      • 5.2 OLIGOMERIC ENZYMES
      • SUMMARY OF CHAPTER 5
  • Part 2: Kinetic and Chemical Mechanisms of Enzyme-Catalysed Reactions
    • 6: An Introduction to Bioenergetics, Catalysis and Kinetics
      • 6.1 SOME CONCEPTS OF BIOENERGETICS
      • 6.2 FACTORS AFFECTING THE RATES OF CHEMICAL REACTIONS
      • 6.3 KINETICS OF UNCATALYSED CHEMICAL REACTIONS
      • 6.4 KINETICS OF ENZYME-CATALYSED REACTIONS: AN HISTORICAL INTRODUCTION
      • 6.5 METHODS USED FOR INVESTIGATING THE KINETICS OF ENZYME-CATALYSED REACTIONS
      • 6.6 THE NATURE OF E

Details

No. of pages:
432
Language:
English
Copyright:
© 2007
Published:
Imprint:
Woodhead Publishing
eBook ISBN:
9780857099921
Print ISBN:
9781904275275

About the authors

P L Bonner

Affiliations and Expertise

Nottingham Trent University, UK

Reviews

Essential. ...explains the structure and function of enzymes. …every chapter tells of an advance in understanding proteins or their interactions. I will definitely use this book., (Review of the first edition) Chemistry & Industry (Professor Carole Hackney, Keele University, UK)
Outstandingly written, updated on molecular biology and bioinformatics, with particularly good treatment of steady-state enzyme kinetics and analytical applications. For undergraduates in biochemistry, biological and analytical sciences, and medicine., (Review of the first edition) Natural Products Report (Royal Society of Chemistry)
An excellent book, warmly recommended., (Review of the first edition) Trends in Biotechnology