Enzymes of Energy Technology, Volume 613

1. Purification of Fully Active and Crystallizable Photosystem II from Thermophilic Cyanobacteria
Keisuke Kawakami and Jian-Ren Shen
2. Production and Manipulation of Blue Copper Oxidases for Technological Applications
Simeng Zhou, Pierre Rousselot-Pailley, Lu Ren, Yolande Charmasson, Elise Courvoisier Dezord, Thierry Tron and Yasmina Mekmouche
3. Production and Spectroscopic Characterisation of Lytic Polysaccharide Monooxygenases
Glyn R. Hemsworth, Luisa Ciano, Gideon J. Davies and Paul H. Walton
4. A Novel Overproduction System for the Structural Determination of a Proton-Pumping Hydrogen-Producing [NiFe]-Hydrogenase
Rhiannon M. Evans and Stephen E. Beaton
5. O2-Tolerant [NiFe]-Hydrogenases of Ralstonia eutropha H16: Physiology, Molecular Biology, Purification, and Biochemical Analysis
Oliver Lenz, Lars Lauterbach and Stefan Frielingsdorf
6. Cytoplasmic and Membrane Bound Hydrogenases from Pyrococcus furiosus
Chang-Hao Wu, Dominik K. Haja and Michael W.W. Adams
7. Purification and Characterization of the [NiFeSe] Hydrogenase from Desulfovibrio vulgaris Hildenborough
Sónia Zacarias, Marisela Vélez, Marcos Pita, António L. De Lacey, Pedro M. Matias and Inês A.C. Pereira
8. [FeFe]-Hydrogenases from Green Algae
Vera Engelbrecht and Thomas Happe
9. Application of Affinity Purification Methods for Analysis of the Nitrogenase System from Azotobacter vinelandii
Emilio Jiménez-Vicente, Julia Sanchez Martin Del Campo, Zhi-Yong Yang, Valerie L. Cash, Dennis R. Dean and Lance C. Seefeldt
10. Membrane-Spanning Electron-Transfer Proteins from Electrogenic Bacteria: Production and Investigation
Colin W.J. Lockwood, Jessica H. van Wonderen, Marcus J. Edwards, Samuel E.H. Piper, Gaye F. White, Simone Newton-Payne, David J. Richardson, Thomas A. Clarke and Julea N. Butt
11. Reductive Activation of Carbon Dioxide by Formate Dehydrogenases
Dimitri Niks and Russ Hille
12. Production and Properties of Enzymes that Activate and Produce Carbon Monoxide
Rodney Burton, Mehmet Can, Daniel Esckilsen, Seth Wiley and Stephen W. Ragsdale
13. Genetic Techniques for Studies of Methyl-Coenzyme M Reductase from Methanosarcina acetivorans C2A
Dipti D. Nayak and William W. Metcalf
14. Navigating Methane Metabolism: Enzymes, Compartments, and Networks
David A. Collins and Marina G. Kalyuzhnaya

Enzymes of Energy Technology, Volume 613 in the Methods in Enzymology series, highlights new advances in the field, with this updated volume presenting interesting chapters written by an international board of authors. Chapters include Purification of fully active and crystallizable photosystem II from thermophilic cyanobacteria, Production and manipulation of [NiFeSe]-hydrogenases for renewable hydrogen research, Hydrogen production by [FeFe]-hydrogenases, Production and properties of enzymes that activate and produce carbon monoxide, Recombinant [NiFe]-hydrogenases from E. coli, Working with nitrogenase, Oxygen--tolerant [NiFe]-hydrogenases, Cytoplasmic and Membrane Bound Hydrogenases from the hyperthermophile Pyrococcus furiosus, and more.

Additional sections cover Enzymatic conversion of methane into useful chemicals, Production and investigations of trans-membrane electron transfer protein, Characterization of post-translational modifications in methyl-coenzyme M reductase in diverse methanogens by mass-spectrometry, Reductive activation of carbon dioxide by formate dehydrogenases, and Lytic polysaccharide monooxygenases in biofuel processing.

14. Production and manipulation of blue copper oxidases for technological applications

Yasmina Mekmouche

• Provides the authority and expertise of leading contributors from an international board of authors
• Presents the latest release in the Methods in Enzymology series
• Includes the latest information on the Enzymes of Energy Technology

Addressed to all specialists in the field