Enzymes in Synthetic Biology - 1st Edition - ISBN: 9780128151488

Enzymes in Synthetic Biology, Volume 608

1st Edition

Serial Volume Editors: Nigel Scrutton
Hardcover ISBN: 9780128151488
Imprint: Academic Press
Published Date: 10th September 2018
Page Count: 412
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Table of Contents

Enzyme Discovery
1. Enzyme selection, cluster discovery and pathway design
Jean-Loup Faulon

Enzyme Engineering Tools and Platforms
2. Cell free systems for biocatalyst discovery and chemically programmed design of biocatalysts
Michael C. Jewett
3. Synthetic biology design and synthesis of new biocatalysts
Douglas Kell and Andrew Currin

Enzyme Families: Parts and Platforms for Chemical Diversity
4. Terpene Cyclases (i): platform catalysts with non-natural substrates
Rudolf Allemann
5. Terpene Cylases (ii): in vivo platforms for generating chemical diversity
Tiangang Liu
6. Amine oxidases/imine reductases for synthetic biology and pathway bioengineering
Nicholas Turner
7. Flavin biocatalytic parts for pathway engineering
Marco Fraaije
8. P450s for synthetic biology applications
Andrew Munro

Genome Engineering for Enzyme Pathways
9. Genome editing for microbial synthetic biology
Huimin Zhao
10. (Automated) yeast genome assembly and implications for large scale pathway assembly
Patrick Cai

Bioengineered Strains with Designed Enzyme Pathways
11. Halomonas and pathway engineering for bio-plastics production
George Guo-Qiang Chen
12. Bioengineered carbon assimilation pathways
Arren-Bar Even
13. Monoterpene production platforms
Nigel Scrutton
14. Commodity chemicals from engineered polyketide synthases
Jay Keasling
15. Modular pathway rewiring of yeast for amino acid production
Jens Nielsen Chalmers


Volume 608 of the series Methods in Enzymology covers key aspects of enzyme discovery, engineering tools and platforms, and examples of applications in the enzymology of synthetic biology.

Key Features

  • Detailed methods for laboratory use of enzymes in synthetic biology applications
  • Informative case history examples illustrating how enzyme and metabolic engineering are used to generate new products
  • Emphasises latest developments in laboratory automation for the engineering of biology
  • Covers many aspects of the design, build, test, learn cycle used in synthetic biology


Relevant to academic and industrial groups working in synthetic biology; students, researchers and teachers.


No. of pages:
© Academic Press 2018
Academic Press
Hardcover ISBN:

Ratings and Reviews

About the Serial Volume Editors

Nigel Scrutton Serial Volume Editor

Nigel Scrutton is Director of the Manchester Institute of Biotechnology (MIB), and Professor of Enzymology and Biophysical Chemistry in the School of Chemistry at the University of Manchester. He has made major contributions to the study of enzyme catalysis, the mechanisms and structures of enzymes and the photochemistry of photoreceptor proteins. His group has pioneered investigations that have led to both deep understanding and recognition of the general importance of quantum tunnelling and protein dynamics in enzyme H-transfer and conformational ensemble sampling in electron transfer reactions. This has involved the development of new biophysical approaches for reaction kinetics analysis including kinetic isotope effect studies, their integration into structural and computational programmes, and extension of theory. He has also made important contributions to enzyme kinetics, coenzyme chemistry, protein engineering, directed evolution, synthetic biology, biological engineering, biocatalysis and metabolic engineering, including the first rational redesign of the coenzyme specificity of an enzyme and the discovery of new riboflavin cofactors. He is currently director of the synthetic biology research centre (SYNBIOCHEM), which specializes in the engineering of biology for chemicals and materials production.

Affiliations and Expertise

The School of Chemistry at the University of Manchester, UK