This volume is a summary of the proceedings of the 8th International Symposium on Vitamin B6 and Carbonyl Catalysis, held in Osaka, Japan, 15-19 October 1990. The development of molecular biology and x-ray crystallography techniques have contributed greatly to the knowledge and understanding of PLP-dependent enzymes. The structural, mechanistic and functional aspects of the PLP-dependent enzymes have been studied for over 40 years by many active researchers. Recent evidence indicates that the coenzyme is substituted by the pyruvoyl group which is a reactive carbonyl in some amino acid decarboxylases, and the presence of pyrroloquinoline quinone (PQQ), another carbonyl coenzyme, in other enzymes. The contents of this volume attest to the exciting progress in elucidating structure, mechanism of action, and the function of enzymes dependent on coenzymes containing the carbonyl group.
For biochemists and chemical enzymologists.
Section headings and selected papers: Plenary Lectures. Biochemical significance of carbonyl catalysis, H Wada et al. Covalent and Spatial Structures of B6 Enzymes. Aspartate aminotransferase from Sulfolobus solfataricus: a hyperthermophilic enzyme, G Marino, et al. Aminotransferases. Cryo-trapping of true enzyme-substrate intermediates of aspartate aminotransferase, M Sterk & H Gehring. Elimination and Replacement Enzymes. Structure and function of tryptophanase from escherichia coli, Y Kawata et al. Other B6 Enzymes. Characterization of 1-aminocyclopropane-1-carboxylate (ACC) synthase, a key enzyme in ethylene biosynthesis: active site peptide sequencing, S F Yang et al. Non-Enzymatic Reactions. Intermediate species absorbing in the 500-nm region in nonenzymatic pyridoxal catalysis, Y Matsushima et al. Phosphorylase - Structure and Mechanism. Mechanism and error rate in phosphorylase catalysis, D Palm et al. Pyruvoyl Enzymes. Enzyme-activated irreversible inhibitors of S-adenosylmethionine decarboxylase, a pyruvoyl enzyme, C Danzin et al. PQQ and Quinones. Trials to detect pyrroloquinoline quinone (PQQ) in bovine plasma amine oxidase and tissues of the rat and human by GAS chromatography/mass spectrometry, T Kumazawa et al. Biological Aspects of Vitamin B6 amd B6 enzymes. Vitamin B6 as an inhibitor of eukaryotic DNA topoisomerase I, P A der Garabedian et al. Metabolism of Vitamin B6. Pyridoxal kinase: inhibitors bound to the pyridoxal binding site, G Scholz et al. New Trends in B6 and Carbonyl Catalysis Study. Reaction of pyridoxamine with carboxylic residues of protein, O-S Kwon & J E Churchich.
2500 lit. refs. approx., 10 halftone illus., 24
- © Pergamon 1991
- 30th August 1991
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Osaka University, Japan