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Enzyme Nanoparticles - 1st Edition - ISBN: 9780323389136, 9780323389297

Enzyme Nanoparticles

1st Edition

Preparation, Characterisation, Properties and Applications

Author: Chandra Pundir
Paperback ISBN: 9780323389136
eBook ISBN: 9780323389297
Imprint: William Andrew
Published Date: 16th May 2015
Page Count: 82
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Table of Contents

  • Dedication
  • About the author
  • Preface
  • Chapter 1. Introduction to Enzyme and Nanotechnology
    • What Are Nanoparticles and Nanotechnology?
    • What Are Enzymes and Their Aggregates?
    • What Are Enzyme Nanoparticles?
    • How Are Nanoparticles of Enzymes Prepared?
    • What Does This Book Describe?
    • References
  • Chapter 2. Preparation of Enzyme Nanoparticles
    • Method of ENPs Formation
    • Chemistry of Formation and Functionalization of ENPs
    • Summary
    • References
  • Chapter 3. Characterization of Enzyme Nanoparticles
    • Summary
    • References
  • Chapter 4. Immobilization of Enzyme Nanoparticles
    • Immobilization of HRP NPs Aggregates
    • Immobilization of GOD NPs Aggregates
    • Immobilization of ChOx NPs Aggregates
    • Immobilization of Uricase NPs Aggregates
    • Chemistry of Immobilization of ENPs
    • Confirmation of Immobilization of ENPs
    • Conjugation Yield and % Retention of Immobilized ENPs
    • Summary
    • References
  • Chapter 5. Kinetic Properties of Enzyme Nanoparticles
    • Optimum pH
    • Optimum Temperature and Thermostability
    • Response Time
    • Km Value
    • Working Range
    • Storage Stability
    • Reusability
    • Summary
    • References
  • Chapter 6. Applications of Enzyme Nanoparticles
    • HorseRadish Peroxidase (HRP) NPs-Based Biosensor
    • Glucose Oxidase (GOD) NPs-Based Biosensors
    • Cholesterol Oxidase (ChOx) NPs-Based Biosensor
    • Uricase NPs-Based Biosensor
    • Summary
    • References
  • Chapter 7. Future Development in Enzyme Nanoparticles
    • References
  • Appendix. Enzymes Used in Day-to-Day Life
    • Statistical Formulae for Precision and Correlation


This book is the first book in English on nanotechnology and nanomaterials integrating with enzymatic systems, with a focus on nanoparticles and biological applications. It covers comprehensively the relevant topics to understand the development of enzyme nanoparticles as it relates to the complicated structures of enzyme nanoparticles and their functionalization and immobilization on to various supports. The preparation of enzyme nanoparticles, their kinetic properties and applications after immobilization of the immobilized enzyme nanoparticles is described. The use of colour images in all formats of the book will improve the understanding of the topics covered. The book offers an integration of Enzymology and Nanotechnology and provides the latest information on preparation of enzyme nanoparticles, their characterization, their functionalization and immobilization on to various supports and thereafter their kinetic properties and applications in various industries with special reference to Biosensor Technology.

Key Features

  • Focus on enzyme nanotechnology, given the wide appeal of enzymes for diagnostics, therapy and biocatalysis
  • Provision of a general background to the topic, but also a detailed description of synthesis, preparation and applications


Researchers in industry and academia, in the fields of Nanoscience and Nanotechnology, Advanced Materials research, Biomedical Engineering, Biotechnology, Bio-Nanotechnology, Biosensor Technology, Nano-Medicine, Pharmacy, Dentistry, Veterinary Medicine, Life Sciences, Enzyme Catalysis and Biochemistry; Graduate and post-graduate students in Biochemistry, Biotechnology, Nanoscience and Nanotechnology, Materials Science, Biosensor Technology and Nano-Medicine courses


No. of pages:
© William Andrew 2015
16th May 2015
William Andrew
Paperback ISBN:
eBook ISBN:

Ratings and Reviews

About the Author

Chandra Pundir

Affiliations and Expertise

Dept. of Biochemistry, Maharshi Dayanand University, Rohtak, Haryana, India